结直肠癌来源外泌体miR-151A-3P对结直肠癌细胞增殖、迁移及患者预后的影响

doi:10.11904/j.issn.1002-3070.2025.02.008

实用肿瘤学杂志 ›› 2025, Vol. 39 ›› Issue (2): 126-133.doi: 10.11904/j.issn.1002-3070.2025.02.008

结直肠癌来源外泌体miR-151A-3P对结直肠癌细胞增殖、迁移及患者预后的影响

刘贺, 潘阳, 梁小龙, 魏正强

重庆医科大学附属第一医院胃肠外科(重庆 400016)

收稿日期:2024-12-25 修回日期:2025-03-24 出版日期:2025-04-28 发布日期:2025-05-06
通讯作者: 魏正强,E-mail:384535713@qq.com
作者简介:刘贺,男,(1990—),博士研究生,住院医师,从事结直肠癌相关研究。

Effects of exosomal miR-151A-3P from colorectal cancer on proliferation,migration and prognosis of colorectal cancer cells

LIU He, PAN Yang, LIANG Xiaolong, WEI Zhengqiang

Department of Gastrointestinal Surgery,The First Affiliated Hospital of Chongqing Medical University,Chongqing 400016, China

Received:2024-12-25 Revised:2025-03-24 Online:2025-04-28 Published:2025-05-06

摘要/Abstract

摘要： 目的探索调控结直肠癌转移的关键基因,研究结直肠癌来源外泌体miR-151A-3P对结直肠癌细胞增殖和迁移的影响,探讨外泌体miR-151A-3P作为结直肠癌患者预后标志物的可能性。方法通过高通量测序筛选出转移性结直肠癌患者血清外泌体差异表达的miRNA,RT-qPCR法验证结直肠癌标本中差异表达miRNA的表达情况;使用mimics过表达或inhibitor干扰miR-151A-3P后,利用CCK-8法和EdU实验检测细胞增殖活力,划痕实验及Transwell实验检测细胞侵袭和迁移能力;裸鼠体内实验评估外泌体miR-151A-3P对结直肠癌细胞转移能力的影响。结果测序结果显示,与非转移性结直肠癌患者相比,转移性结直肠癌患者血清外泌体中miR-151A-3P的表达增高(P＜0.001)。RT-qPCR检测验证了该结果,发现miR-151A-3P在转移性结直肠癌患者的血清外泌体和肿瘤组织中高表达(P＜0.001),并且血清外泌体miR-151A-3P高表达的结直肠癌患者总生存期短于低表达结直肠癌患者(P=0.029)。沉默miR-151A-3P后,肿瘤细胞增殖、侵袭、迁移能力下降(P＜0.05),而过表达miR-151A-3P后能够促进肿瘤细胞的增殖、侵袭和迁移(P＜0.05)。在BALB/c裸鼠结直肠癌模型中,给予外泌体miR-151A-3P导致裸鼠肺转移数量增多(P＜0.05)。结论结直肠癌来源外泌体miR-151A-3P促进结直肠癌细胞增殖和迁移,并与结直肠癌患者不良预后相关。

关键词: 结直肠癌, 外泌体, miR-151A-3P, 增殖, 迁移

Abstract: Objective The aim of this study was to investigate the key genes that regulate colorectal cancer(CRC)metastasis,evaluate the effects of exosomal miR-151A-3P derived from CRC on the proliferation and migration,and explore the possibility of exosomal miR-151A-3P as a prognostic biomarker for CRC patients. Methods High-throughput sequencing was performed to screen the differentially expressed miRNAs in serum exosomes of metastatic CRC patients.RT-qPCR was used to verify the expression of differentially expressed miRNAs in CRC specimens.After mimics overexpression or knockdown of miR-151A-3P,CCK-8 and EdU assays were used to detect cell proliferation,and Scratch wound healing and Transwell assays were used to detect cell migration and invasion capability.A BALB/c nude mouse model was used to evaluate the effect of exosomal miR-151A-3P on the metastatic ability of CRC cells. Results The sequencing results showed that compared with non-metastatic CRC patients,the expression of miR-151A-3P was significantly increased in serum exosomes of metastatic CRC patients(P＜0.001).The results were validated by RT-qPCR and found that miR-151A-3P was highly expressed in both serum exosomes and tumor tissues of metastatic CRC patients(P＜0.001).The overall survival of CRC patients with high expression of serum exosomal miR-151A-3P was shorter than that of CRC patients with low expression(P=0.029).After silencing miR-151A-3P,the cell proliferation,migration,and invasion abilities of CRC cells were decreased(P＜0.05),while overexpression of miR-151A-3P could promote the proliferation,migration and invasion of CRC cells(P＜0.05).In the BALB/c nude mouse colorectal cancer model,the administration of exosomal miR-151A-3P led to an increase in the number of lung metastases in BALB/c nude mice(P＜0.05). Conclusion CRC-derived exosomal miR-151A-3P promotes the proliferation and migration of CRC cells,and is associated with poor prognosis of CRC patients.

Key words: Colorectal cancer, Exosome, miR-151A-3P, Proliferation, Migration

中图分类号:

R735.3+4

刘贺, 潘阳, 梁小龙, 魏正强. 结直肠癌来源外泌体miR-151A-3P对结直肠癌细胞增殖、迁移及患者预后的影响[J]. 实用肿瘤学杂志, 2025, 39(2): 126-133.

LIU He, PAN Yang, LIANG Xiaolong, WEI Zhengqiang. Effects of exosomal miR-151A-3P from colorectal cancer on proliferation,migration and prognosis of colorectal cancer cells[J]. Journal of Practical Oncology, 2025, 39(2): 126-133.

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结直肠癌来源外泌体miR-151A-3P对结直肠癌细胞增殖、迁移及患者预后的影响

Effects of exosomal miR-151A-3P from colorectal cancer on proliferation,migration and prognosis of colorectal cancer cells

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