膀胱癌组织中MMP-2与TIMP-2 mRNA的表达及临床意义

doi:10.3969/j.issn.1002-3070.2010.01.007

实用肿瘤学杂志 ›› 2010, Vol. 24 ›› Issue (1): 29-32.doi: 10.3969/j.issn.1002-3070.2010.01.007

膀胱癌组织中MMP-2与TIMP-2 mRNA的表达及临床意义

王云炎, 侯建全, 温端改

苏州大学第一附属医院泌尿外科(苏州 215006)

收稿日期:2009-11-25 出版日期:2010-02-28 发布日期:2015-01-23
作者简介:王云炎,男,(1977-),博士,主治医师,从事泌尿生殖系肿瘤的基础与临床研究

The expression and clinical significance of MMP-2 and TIMP-2 mRNA in bladder transitional cell carcinoma

WANG Yunyan, HOU Jianquan, WEN Duangai

Department of Urology,the First Hospital Affiliated to Soochow University,Suzhou 215006

Received:2009-11-25 Online:2010-02-28 Published:2015-01-23

摘要/Abstract

摘要： 目的探讨基质金属蛋白酶-2(MMP-2)及其组织抑制因子-2(TIMP-2)mRNA在膀胱癌组织中的表达,以及与膀胱癌侵袭、转移和复发的关系。方法应用半定量PCR和实时定量PCR(RQ-PCR)方法,检测10例正常膀胱组织和45例膀胱移行细胞癌组织中MMP-2和TIMP-2 mRNA的表达。结果半定量PCR显示MMP-2、TIMP-2在膀胱正常组织中均呈阴性表达,而在45例膀胱癌组织中,MMP-2阳性率为66.7%(30/45),TIMP-2为57.8%(26/45)。定量PCR检测显示MMP-2表达量随肿瘤病理分级和临床分期的升高而增加,在复发病例中也增加,其组间差异有统计学意义(P＜0.05);TIMP-2的表达量随病理分级和临床分期的升高而降低,而且组间差异有统计学意义(P＜0.05),而在初发复发病例的组间差异无统计学意义(P＞0.05)。结论 MMP-2和TIMP-2对于膀胱癌的侵袭、转移和复发发挥了重要作用;MMP-2可能对判断膀胱癌的预后有一定的指导意义。

Abstract: Objective To investigate the expression of MMP-2 and TIMP-2 mRNA in transitional cell carcinoma of bladder and discuss their clinical significance.Methods Using RT-PCR and real time quantitative PCR(RQ-PCR)technique,the expression of MMP-2 and TIMP-2 mRNA of 45 cases of bladder carcinoma(tumor group)and 10 cases of normal bladder tissue(control group)were analyzed.Results MMP-2 and TIMP-2 were not expressed in control group.MMP-2 was expressed in 30 cases tumor samples and TIMP-2 was expressed in 26 cases.The expression of MMP-2 mRNA in superficial bladder cancer and G1 cancer were lower than that in infiltrating bladder cancer and G2-G3 cancer respectively.The differences were statistically significant(P ＜0.05).The expression of MMP-2 in recurrence group was higher than that in incipient group.TIMP-2 mRNA expression decreased with grades and stage.The expression of TIMP-2 in superficial bladder cancer and G1 cancer were higher than that in infiltrating bladder cancer and G2-G3 cancer respectively.There was statistical difference between two groups(P ＜0.05).TIMP-2 expression in incipient group was higher than that in recurrence group,but the difference was not significant(P＞0.05).Conclusion These results suggest that MMP-2 and TIMP-2 play an important role in the invasive step of transitional cell carcinoma of bladder.MMP-2 may become a new approach to the diagnosis of bladder carcinomas.

中图分类号:

R73-37

王云炎, 侯建全, 温端改. 膀胱癌组织中MMP-2与TIMP-2 mRNA的表达及临床意义[J]. 实用肿瘤学杂志, 2010, 24(1): 29-32.

WANG Yunyan, HOU Jianquan, WEN Duangai. The expression and clinical significance of MMP-2 and TIMP-2 mRNA in bladder transitional cell carcinoma[J]. PRACTICAL ONCOLOGY JOURNAL, 2010, 24(1): 29-32.

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膀胱癌组织中MMP-2与TIMP-2 mRNA的表达及临床意义

The expression and clinical significance of MMP-2 and TIMP-2 mRNA in bladder transitional cell carcinoma

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