CCNB1基因通过调控PI3K/Akt信号通路对口腔鳞癌细胞增殖、侵袭和迁移的影响

doi:10.11904/j.issn.1002-3070.2020.02.011

摘要/Abstract

摘要： 目的探讨细胞周期蛋白B1(CCNB1)基因对口腔鳞癌细胞增殖、侵袭和迁移的影响及作用机制。方法实时荧光定量PCR(qRT-PCR)检测人正常口腔上皮细胞系HOK和人口腔鳞癌细胞系SCC-15、SCC-4、Cal-27中CCNB1基因的表达量,将CCNB1 siRNA(si-CCNB1)和阴性对照(si-NC)转染至SCC-15细胞,同时设置空白对照(Control),qRT-PCR和Western blot检测各组SCC-15细胞中CCNB1的表达,MTT实验、Transwell实验和划痕实验分别检测沉默CCNB1对SCC-15细胞增殖、侵袭和迁移能力的影响。Western blot检测细胞中MMP-2、MMP-9、PI3K、Akt和p-Akt蛋白的表达。结果 CCNB1在口腔鳞癌细胞系中的表达显著高于正常口腔上皮细胞(P＜0.05),si-NC组SCC-15细胞中CCNB1 mRNA和蛋白的表达与Control组无统计学差异(P＞0.05);si-CCNB1组中CCNB1 mRNA和蛋白的表达明显低于si-NC组和Control组(P＜0.05),si-NC组SCC-15细胞增殖、侵袭和迁移能力及MMP-2、MMP-9、PI3K、Akt、p-Akt蛋白表达与Control组无统计学差异(P＞0.05);si-CCNB1组细胞增殖、侵袭和迁移能力及MMP-2、MMP-9、PI3K、p-Akt蛋白表达均明显低于si-NC组和Control组(P＜0.05),两组间Akt蛋白表达无统计学差异(P＞0.05)。结论 CCNB1在口腔鳞癌细胞系中呈高表达,沉默CCNB1基因能够抑制人口腔鳞癌SCC-15细胞增殖、侵袭和迁移,其作用机制可能与抑制PI3K/Akt信号通路的激活有关。

关键词: CCNB1基因, PI3K/Akt信号通路, 口腔鳞癌细胞, 增殖, 侵袭, 迁移

Abstract: Objective The aim of this study was to investigate the effect and mechanism of cyclin B1(CCNB1)gene on the proliferation,invasion and migration of oral squamous cell carcinoma.Methods Quantitative real-time fluorescent PCR(qRT-PCR)was used to detect the expression of CCNB1 gene in human normal oral epithelial HOK cell line and human oral squamous cell carcinoma SCC-15,SCC-4 and Gal-27 cell lines.CCNB1 siRNA(si-CCNB1)and negative control(si-NC)were transfected into SCC-15 cells.At the same time,blank control(Control)was set.qRT-PCR and Western blot were used to detect the expression of CCNB1 in each group of SCC-15 cells.MTT,Transwell and Scratch assays were used to detect the effects of silencing CCNB1 on the proliferation,invasion and migration of SCC-15 cells.Western blot was used to detect the expression of MMP-2,MMP-9,PI3K,Akt and p-Akt in SCC-15 cells.Results The expression of CCNB1 in oral squamous carcinoma cells was significantly higher than that in normal oral epithelial cells(P＜0.05).The expression of CCNB1 mRNA and protein in SCC-15 cells in the si-NC group was not different from that of the control group(P＞0.05),The expression of CCNB1 mRNA and protein in the si-CCNB1 group was significantly lower than that in the si-NC group and control group(P＜0.05),The proliferation,invasion and migration ability of SCC-15 cells in the si-NC group and the expression of MMP-2,MMP-9,PI3K,Akt and p-Akt in the si-NC group were not significantly different from those in the control group(P＞0.05).The cell proliferation,invasive and migratory abilities,and the expressions of MMP-2,MMP-9,PI3K and p-Akt in the si-CCNB1 group were significantly lower than those in the si-NC group and control group(P＜0.05).There was no difference in the expression of Akt protein between the si-CCNB1 and si-NC groups(P＞0.05).Conclusion CCNB1 is highly expressed in oral squamous carcinoma cells.Silencing CCNB1 gene can inhibit the proliferation,invasion and migration of human oral squamous carcinoma SCC-15 cells.The mechanism may be related to the inhibitory activation of PI3K/Akt signaling pathway.

Key words: CCNB1 gene, PI3K/Akt signaling pathway, Oral squamous cell carcinoma, Proliferation, Invasion, Migration

中图分类号:

R739.8

王璞, 韦丽宾, 倪广晓. CCNB1基因通过调控PI3K/Akt信号通路对口腔鳞癌细胞增殖、侵袭和迁移的影响[J]. 实用肿瘤学杂志, 2020, 34(2): 144-149.

WANG Pu, WEI Libin, NI Guangxiao. Effect of CCNB1 gene on proliferation,invasion and migration of oral squamous cell carcinoma cells by regulating PI3K/Akt signaling pathway[J]. Journal of Practical Oncology, 2020, 34(2): 144-149.

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链接本文: http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/10.11904/j.issn.1002-3070.2020.02.011

http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/Y2020/V34/I2/144

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