液体活检在结直肠癌诊断中的应用进展

doi:10.11904/j.issn.1002-3070.2020.04.012

摘要/Abstract

摘要： 结直肠癌(Colorectal cancer,CRC)作为全球第三大常见癌症,是导致人类死亡的重要原因之一。目前针对CRC的诊断手段仍在不断完善,液体活检作为一种新兴的无创活检组织检查技术,受到广泛关注。液体活检主要对生物源液体特定成分进行分析,其中主要包括循环肿瘤细胞、循环肿瘤DNA、细胞游离DNA、外泌体、肿瘤相关血小板及循环肿瘤相关微粒等。本文就以上生物源液体特定成分在CRC诊断中的最新应用研究进行综述。

关键词: 结直肠癌, 液体活检, 诊断

Abstract: Colorectal cancer(CRC),as the third most common cancer in the world,is one of important cause of human death.At present,the diagnostic methods for CRC are still being improved.Liquid biopsy,as an emerging non-invasive biopsy technology,has attracted widespread attention.Liquid biopsy mainly analyzes specific components of biological source liquid,which mainly include circulating tumor cells,circulating tumor DNA,cell free DNA,exosomes,tumor-educated platelets,and circulating tumor-associated microparticles.This article reviews the latest application research of the above biological source liquid specific components in the diagnosis of CRC.

Key words: Colorectal cancer, Liquid biopsy, Diagnosis

中图分类号:

李恒震, 张艳桥. 液体活检在结直肠癌诊断中的应用进展[J]. 实用肿瘤学杂志, 2020, 34(4): 357-361.

LI Hengzhen, ZHANG Yanqiao. Application progress of liquid biopsy in the diagnosis of colorectal cancer[J]. Journal of Practical Oncology, 2020, 34(4): 357-361.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/10.11904/j.issn.1002-3070.2020.04.012

http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/Y2020/V34/I4/357

参考文献

1 Arnold M,Sierra MS,Laversanne M,et al.Global patterns and trends in colorectal cancer incidence and mortality[J].Gut,2016,66(4):683-691.
2 Simon K.Colorectal cancer development and advances in screening[J].Clin Interv Aging,2016,11:967-976.
3 Scholer LV,Reinert T,Orntoft MW,et al.Clinical implications of monitoring circulating tumor DNA in patients with colorectal cancer[J].Clin Cancer Res,2017,23(18):5437-5445.
4 Wan JCM,Massie C,Garcia-Corbacho J,et al.Liquid biopsies come of age:towards implementation of circulating tumour DNA[J].Nat Rev Cancer,2017,17(4):223-238.
5 Stroun M,Maurice P,Vasioukhin V,et al.The origin and mechanism of circulating DNA[J].Ann N Y Acad Sci,2010,906(1):161-168.
6 Yue C,Jiang Y,Li P,et al.Dynamic change of PD-L1 expression on circulating tumor cells in advanced solid tumor patients undergoing PD-1 blockade therapy[J].Oncoimmunology,2018,7(7):e1438111.
7 Siravegna G,Marsoni S,Siena S,et al.Integrating liquid biopsies into the management of cancer[J].Nat Rev Clin Oncol,2017,14(9):531-548.
8 崔雪阳,邢承忠.液体活检在结直肠癌领域的研究及应用现状[J].实用肿瘤学杂志,2018,32(4):85-88.
9 Lee JH,Long GV,Menzies AM,et al.Association between circulating tumor DNA and pseudoprogression in patients with metastatic melanoma treated with anti-programmed cell death 1 antibodies[J].JAMA Oncol,2018,4(5):717-721.
10 Olsson E,Winter C,George A,et al.Serial monitoring of circulating tumor DNA in patients with primary breast cancer for detection of occult metastatic disease[J].Embo Mol Med,2015,7(8):1034-1047.
11 Parkinson CA,Gale D,Piskorz AM,et al.Exploratory analysis of TP53 mutations in circulating tumour DNA as biomarkers of treatment response for patients with relapsed high-grade serous ovarian carcinoma:a retrospective study[J].PLoS Med,2016,13(12):e1002198.
12 Luo HY,Zhao Q,Wei W,et al.Circulating tumor DNA methylation profiles enable early diagnosis,prognosis prediction,and screening for colorectal cancer[J].Sci Transl Med,2020,12(524):7533.
13 Wan N,Weinberg D,Liu TY,et al.Machine learning enables detection of early-stage colorectal cancer by whole-genome sequencing of plasma cell-free DNA[J].BMC Cancer,2019,19(1):832.
14 Church TR,Wandell M,Lofton-Day C,et al.Prospective evaluation of methylated SEPT9 in plasma for detection of asymptomatic colorectal cancer[J].Gut,2014,63(2):317-325.
15 Ludwig AK,Giebel B.Exosomes:small vesicles participating in intercellular communication[J].Int J Biochem Cell Biol,2012,44(1):11-15.
16 Ogata-Kawata H,Izumiya M,Kurioka D,et al.Circulating exosomal microRNAs as biomarkers of colon cancer[J].PLoS One,2014,9(4):e92921.
17 Hu HY,Yu CH,Zhang HH,et al.Exosomal miR-1229 derived from colorectal cancer cells promotes angiogenesis by targeting HIPK2[J].Int J Biol Macromol,2019,132:470-477.
18 Fu F,Jiang W,Zhou L,et al.Circulating exosomal miR-17-5p and miR-92a-3p predict pathologic stage and grade of colorectal cancer[J].Transl Oncol,2018,11(2):221-232.
19 Uratani R,Toiyama Y,Kitajima T,et al.Diagnostic potential of cell-free and exosomal MicroRNAs in the identification of patients with high-risk colorectal adenomas[J].PLoS One,11(10):e0160722.
20 Barbagallo C,Brex D,Caponnetto A,et al.lncRNA UCA1,up regulated in CRC biopsies and downregulated in serum exosomes,controls mRNA expression by RNA-RNA interactions[J].Mol Ther Nucleic Acids,2018,12:229-241.
21 Cooke NM,Egan K,McFadden S,et al.Increased platelet reactivity in patients with late-stage metastatic cancer[J].Cancer Med,2013,2(4):564-570.
22 Best MG,Wesseling P,Wurdinger T.Tumor-educated platelets as a noninvasive biomarker source for cancer detection and progression monitoring[J].Cancer Res,2018,78(13):3407-3412.
23 Best MG,Vancura A,Wurdinger T.Platelet RNA as a circulating biomarker trove for cancer diagnostics[J].Thromb Haemost,2017,15(7):1295-1306.
24 Lin RJ,Afshar-Kharghan V,Schafer AI.Paraneoplastic thrombocytosis:the secrets of tumor self-promotion[J].Blood,2014,124(2):184-187.
25 Sabrkhany S,Kuijpers MJE,Van Kuijk SMJ,et al.A combination of platelet features allows detection of early-stage cancer[J].Eur J Cancer,2017,80:5-13.
26 Best MG,Sol N,Kooi I,et al.RNA-Seq of tumor-educated platelets enables blood-based pan-cancer,multiclass,and molecular pathway cancer diagnostics[J].Cancer Cell,2015,28(5):666-676.
27 Yang L,Jiang Q,Li DZ,et al.TIMP1 mRNA in tumor-educated platelets is diagnostic biomarker for colorectal cancer[J].Aging,2019,11(20):8998-9012.
28 Sol N,Wurdinger T.Platelet RNA signatures for the detection of cancer[J].Cancer Metastasis Rev,2017,36(2):263-272.
29 Piccin A,Murphy WG,Smith OP.Circulating microparticles:pathophysiology and clinical implications[J].Blood Rev,2007,21(3):157-171.
30 Van Doormaal FF,Kleinjan A,Di Nisio M,et al.Cell-derived microvesicles and cancer[J].Neth J Med,2009,67(7):266-273.
31 Rak J.Microparticles in cancer[J].Seminars Thromb Hemost,2010,36(8):888-906.
32 D'Souza-Schorey C,Clancy JW.Tumor-derived microvesicles:shedding light on novel microenvironment modulators and prospective cancer biomarkers[J].Genes Dev,2012,26(12):1287-1299.
33 Willms A,Müller C,Julich H,et al.Tumour-associated circulating microparticles:a novel liquid biopsy tool for screening and therapy monitoring of colorectal carcinoma and other epithelial neoplasia[J].Oncotarget,2016,7(21):30867-30875.

[1]	赵仁成, 林子棠, 余卫军, 雷林, 刘峥, 徐英, 郭艳芳, 彭绩. 2017—2019年深圳市宝安区40~74岁常住居民五癌风险流行状况分析[J]. 实用肿瘤学杂志, 2020, 34(4): 315-320.
[2]	张锐, 李志高. 粘液性乳腺癌的临床研究进展[J]. 实用肿瘤学杂志, 2020, 34(4): 377-381.
[3]	朱晓云, 张亚宁, 马碧萍. 1980—2018年上海市金山区结直肠癌死亡水平及其所致早死概率的趋势分析[J]. 实用肿瘤学杂志, 2020, 34(3): 198-202.
[4]	鲁雪梅, 李云涛, 安锦慧, 季国忠. 溶血磷脂酸在结直肠癌发生发展中的机制研究[J]. 实用肿瘤学杂志, 2020, 34(3): 276-281.
[5]	李斌, 冯保恒, 尹富霞, 苏伟. 建立结直肠癌移植瘤模型研究小鼠肠道中细菌感染情况及地西他滨联合顺铂化疗的作用机制[J]. 实用肿瘤学杂志, 2020, 34(2): 163-168.
[6]	曾理, 庄树彤, 丁世华, 陈冲, 焦璐, 孙大勇. 长链非编码RNA AFAP1-AS1通过PTEN/p-AKT信号通路调控结直肠癌细胞增殖的分子机制研究[J]. 实用肿瘤学杂志, 2020, 34(1): 11-16.
[7]	廖代祥, 郑博, 张彦斌, 于军辉. RRBP1与RABEX-5蛋白在结直肠癌组织中的表达及与临床病理特征和预后的关系[J]. 实用肿瘤学杂志, 2020, 34(1): 43-48.
[8]	张景岚, 刘瑞宝. 结直肠癌肝转移瘤局部介入治疗应用进展[J]. 实用肿瘤学杂志, 2020, 34(1): 79-82.
[9]	李智杰, 赵琦, 吴华星. 通过宏基因组学研究肠道微生物对结直肠癌患者影响的最新进展[J]. 实用肿瘤学杂志, 2020, 34(1): 93-96.
[10]	夏天一, 张博淼, 姚克非, 崔滨滨. PTPN9表达水平与结直肠癌的临床关系研究[J]. 实用肿瘤学杂志, 2019, 33(5): 415-419.
[11]	李思维 , 庞达. 长链非编码RNA(lncRNA)在癌症中的作用[J]. 实用肿瘤学杂志, 2019, 33(5): 471-475.
[12]	方瑄, 陈洪生, 李国东刘明. 异常隐窝灶治疗的研究进展[J]. 实用肿瘤学杂志, 2019, 33(4): 376-379.
[13]	孙江宏, 郝明珠, 姜丹, 国飞, 李晓梅. 乳腺炎、良性增生及乳腺癌FFDM鉴别诊断思路[J]. 实用肿瘤学杂志, 2019, 33(2): 139-141.
[14]	方琳, 刘超, 张纯慧, 张艳桥. 转移性结直肠癌维持治疗的研究现状与治疗策略[J]. 实用肿瘤学杂志, 2019, 33(2): 173-178.
[15]	佟广海, 田洋, 吴华星. 脂质组学在结直肠癌研究中的应用[J]. 实用肿瘤学杂志, 2019, 33(2): 179-192.