Hsa-let-7b-5p抑制非小细胞肺癌的增殖、迁移和侵袭

doi:10.11904/j.issn.1002-3070.2024.03.003

摘要/Abstract

摘要： 目的探讨hsa-let-7b-5p在非小细胞肺癌临床诊断和靶向治疗中的价值。方法收集2020年9月-10月的13对哈尔滨医科大学附属肿瘤医院病理类型相同的非小细胞肺癌癌组织及其癌旁组织,通过RNA-Seq获得差异表达的miRNA,并通过qRT-PCR验证候选miRNA在非小细胞肺癌组织和细胞中的表达水平。通过CCK-8法、克隆形成实验、划痕实验和Transwell实验验证miRNA对非小细胞肺癌增殖、迁移和侵袭能力的影响。利用MiRDB、TarBase和ENCORI数据库预测靶基因,并利用FunRich工具进行基因富集分析。结果与癌旁组织及正常支气管上皮细胞系(Human bronchial epithelial cell,HBE)相比,hsa-let-7b-5p在非小细胞肺癌组织及非小细胞肺癌细胞系(A549、H1299、H358和H460)中低表达(P＜0.05)。hsa-let-7b-5p的高表达可以抑制非小细胞肺癌增殖、迁移和侵袭能力(P＜0.01)。靶基因预测及富集分析结果显示hsa-let-7b-5p的378个靶基因及其相关基因中,147个基因与hsa-let-7b-5p的表达呈负相关(P＜0.05)。结论 Hsa-let-7b-5p对非小细胞肺癌的增殖、迁移和侵袭有抑制作用。

关键词: hsa-let-7b-5p, 非小细胞肺癌, 增殖, 迁移, 侵袭, 生物标志物

Abstract: Objective The objective of this study was to explore the values of hsa-let-7b-5p in the clinical diagnosis and targeted therapy of non-small cell lung cancer(NSCLC). Methods Thirteen pairs of NSCLC tissues and their adjacent tissues with the same pathological type in Harbin Medical University Cancer Hospital from September to October 2020 were collected,and differentially expressed miRNAs were obtained by RNA-Seq,and the expression of candidate miRNAs were verified in NSCLC tissues and cells through qRT-PCR.The effects of miRNAs on the proliferation,migration and invasion ability of NSCLC were verified by CCK-8 assay,clone formation assay,scratch assay and Transwell assay.Target genes were predicted using MiRDB,TarBase and ENCORI databases,and gene enrichment analysis was performed using FunRich tool. Results Compared with adjacent tissues and human bronchial epithelial cells(HBE),hsa-let-7b-5p was downregulated in NSCLC tissues and cell lines(A549 cells,H1299 cells,H358 cells and H460 cells)(P＜0.05).The high expression of hsa-let-7b-5p inhibited the proliferation,migration and invasion ability of NSCLC(P＜0.01).The results of target genes and enrichment analysis showed that among 378 target genes and related genes of hsa-let-7b-5p,147 genes were negatively correlated with the expression of hsa-let-7b-5p(P＜0.05). Conclusion Hsa-let-7b-5p has inhibitory effects on the proliferation,migration and invasion of NSCLC.

Key words: Hsa-let-7b-5p, Non-small cell lung cancer, Proliferation, Migration, Invasion, Biomarker

中图分类号:

R734.2

程泳铭, 董欢欢, 徐晖. Hsa-let-7b-5p抑制非小细胞肺癌的增殖、迁移和侵袭[J]. 实用肿瘤学杂志, 2024, 38(3): 157-167.

CHENG Yongming, DONG Huanhuan, XU Hui. Hsa-let-7b-5p inhibits proliferation,migration and invasion in non-small cell lung cancer[J]. Journal of Practical Oncology, 2024, 38(3): 157-167.

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