TRIM5α对肝癌细胞增殖和凋亡的影响

doi:10.11904/j.issn.1002-3070.2016.02.002

Abstract

Abstract: Objective To study the effect of Tripartite motif 5 alpha(TRIM5α)on the proliferation and apoptosis of hepatocellular carcinoma cells.Methods The TRIM5α construction was identified by enzyme digestion,PCR and sequencing.The TRIM5α plasmid was transfected into HepG2 cells and identified by RT-PCR and Western blot.The cell proliferation was monitored by RTCA real-time instrument and cell apoptosis was analyzed by flow cytometry.The apoptosis related proteins were detected by Western blot.Results The TRIM5α vector was successfully constructed.The result of RT-PCR and Western blot showed that TRIM5α plasmid could enter HepG2 cells.TRIM5α could inhibit HepG2 cells proliferation also increased their apoptosis through down-regulation of Bcl-2 expression and activation of Caspase-3 expression.TRIM5α also could inhibit the tumor formation of HepG2 cells in vivo.Conclusion TRIM5α inhibited cell proliferation,promoted apoptosis and weakened the tumorigenic ability of HepG2 cells.This will establish the foundation for the mechanism study and the clinic use of TRIM5α for tumor therapy in the future.

Key words: Tripartite motif 5 alpha, Hepatocellular carcinoma cells, Proliferation, Apoptosis

CLC Number:

R73.3

YU Qing, ZHANG Wenmei, SUN Yingying, GONG Yuanyuan, LI Dongsheng, WANG Xiaoli. The effect of TRIM5α on the proliferation and apoptosis of hepatocellular carcinoma cells[J]. PRACTICAL ONCOLOGY JOURNAL, 2016, 30(2): 103-108.

References

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The effect of TRIM5α on the proliferation and apoptosis of hepatocellular carcinoma cells

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