Wnt通路抑制剂ETC-159处理对口腔鳞癌细胞增殖迁移的影响

doi:10.11904/j.issn.1002-3070.2018.02.002

Abstract

Abstract:

Objective The Objective of this study was to investigate the effect of Wnt pathway inhibitor-ETC-159 on proliferation and migration of oral squamous cell carcinoma(SCC-15)cells and to explore its mechanism.Methods SCC-15 cells were treated with DMSO and ETC-159 for 12 h or 24 h.Cell proliferation was detected by CCK8 kit.Transwell assay was used to detect the ability of cell migration.Western blotting was used to detect cell migration related to proteins i.e.Wnt3a and β-catenin,proliferation and migration related proteins i.e.c-Myc,cyclin D1,CD146.Results After treated with ETC-159 for 12 or 24 h,the proliferation,migration and expression of Wnt3a,β-catenin,c-Myc,cyclin D1 and CD146 in SCC-15 cells were significantly decreased when compared to the DMSO group(P＜0.05).Conclusion Wnt signaling pathway inhibitor-ETC-159 can inhibit the proliferation and migration of SCC-15 cells by decreased levels of c-Myc,cyclin D1 and CD146.

Key words: Oral squamous cell carcinoma, ETC-159, Wnt pathway, Proliferation, Migration

CLC Number:

R739.85

DUAN Yanjun, XU Wei, CHEN Wenjie, WANG Fengjuan. Effects of Wnt pathway inhibitor ETC-159 on proliferation and migration of OSCC cells[J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(2): 103-106.

References

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Effects of Wnt pathway inhibitor ETC-159 on proliferation and migration of OSCC cells

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