Abstract: Objective The inhibitory effect of the PARP inhibitor olaparib on human acute myeloid leukemia HL-60 cells was studied.Methods The HL-60 cells in logarithmic growth phase were treated with different concentrations(1.25,2.5,5 and 10 μmol/L)of olaparib for different time.The CCK-8 assay was used to detect the inhibitory effect of olaparib on HL-60 cells.The apoptotic level of HL-60 cells was detected by Annexin-V/PI double staining method,and the expression of related signal proteins(PARP-1 and caspase-3)in HL-60 cells was detected by Western blot.Results HL-60 cells were inhibited by olaparib at different concentrations(1.25,2.5,5 and 10 μmol/L)for 48 h,and the inhibition rate gradually increased with the prolongation of the action time;at the same time,the apoptotic rate was increased in HL-60 cells after olaparib treatment for 48 h,showing a dose-dependent manner;the PARP activity was inhibited and caspase-3 was activated in HL-60 cells treated with olaparib.Conclusion The PARP inhibitor olaparib not only inhibits proliferation of HL-60 cells,but it also promotes apoptosis of HL-60 cells by inhibiting PARP activity and activating caspase-3.

Key words: Olaparib, PARP inhibitor, HL-60 cells, Acute myeloid leukemia