ZCCHC12对甲状腺细胞恶性转化的影响与作用机制

doi:10.11904/j.issn.1002-3070.2020.02.009

Abstract

Abstract: Objective The aims of this study were to analyze the expression of zinc finger protein 12(ZCCHC12)containing CCHC domain in thyroid cancer tissues and cells,and to explore its effect and mechanism on malignant transformation of thyroid cells.Methods The expression of ZCCHC12 in tumor tissues from 30 thyroid cancer patients,10 benign thyroid tissues and cancer cell lines were detected by qRT-PCR and Western blot.ZCCHC12 adenovirus vector or siRNA was used to transfect thyroid cancer cells,and the cell proliferation,invasion ability and expression of corresponding molecules were detected.Results Compared with benign thyroid tissues,the expression of ZCCHC12 in thyroid cancer tissues increased(P＜0.05).At the same time,compared with thyroid follicular epithelial HUM-CELL-0097 cells,the expression of ZCCHC12 at levels of mRNA and protein in thyroid cancer cells also significantly increased(P＜0.05).After ZCCHC12 overexpression,the proliferation and invasive ability of thyroid cancer cells increased significantly(P＜0.05).On the contrary,the proliferation and invasion ability of ZCCHC12 was inhibited after ZCCHC12 silencing(P＜0.05).Compared with the control group,the expression of cyclin D,matrix metalloproteinase 2(MMP-2)and MMP-9 increased after ZCCHC12 overexpression and their expression decreased after ZCCHC12 silencing(P＜0.05).Conclusion ZCCHC12 is highly expressed in thyroid cancer tissues and thyroid cancer cells,and its proliferative activity and invasion ability increase after overexpression ZCCHC12.The abnormal expression of ZCCHC12 can activate the expression of cell cycle related proteins and extracellular matrix related enzymes,and aggravate the malignant transformation of thyroid cells.

Key words: ZCCHC12, Thyroid cancer, Proliferation, Invasive, Molecular mechanism

CLC Number:

R730.23

LIU Guangxia, ZHAO Lianchun, CHEN Fang, JIA Qian, GAO Wei, HOU Zhan, LU Yamin. Effect and mechanism of ZCCHC12 on malignant transformation of thyroid cells[J]. Journal of Practical Oncology, 2020, 34(2): 133-138.

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URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2020.02.009

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2020/V34/I2/133

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Effect and mechanism of ZCCHC12 on malignant transformation of thyroid cells

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