CCNB1基因通过调控PI3K/Akt信号通路对口腔鳞癌细胞增殖、侵袭和迁移的影响

doi:10.11904/j.issn.1002-3070.2020.02.011

Abstract

Abstract: Objective The aim of this study was to investigate the effect and mechanism of cyclin B1(CCNB1)gene on the proliferation,invasion and migration of oral squamous cell carcinoma.Methods Quantitative real-time fluorescent PCR(qRT-PCR)was used to detect the expression of CCNB1 gene in human normal oral epithelial HOK cell line and human oral squamous cell carcinoma SCC-15,SCC-4 and Gal-27 cell lines.CCNB1 siRNA(si-CCNB1)and negative control(si-NC)were transfected into SCC-15 cells.At the same time,blank control(Control)was set.qRT-PCR and Western blot were used to detect the expression of CCNB1 in each group of SCC-15 cells.MTT,Transwell and Scratch assays were used to detect the effects of silencing CCNB1 on the proliferation,invasion and migration of SCC-15 cells.Western blot was used to detect the expression of MMP-2,MMP-9,PI3K,Akt and p-Akt in SCC-15 cells.Results The expression of CCNB1 in oral squamous carcinoma cells was significantly higher than that in normal oral epithelial cells(P＜0.05).The expression of CCNB1 mRNA and protein in SCC-15 cells in the si-NC group was not different from that of the control group(P＞0.05),The expression of CCNB1 mRNA and protein in the si-CCNB1 group was significantly lower than that in the si-NC group and control group(P＜0.05),The proliferation,invasion and migration ability of SCC-15 cells in the si-NC group and the expression of MMP-2,MMP-9,PI3K,Akt and p-Akt in the si-NC group were not significantly different from those in the control group(P＞0.05).The cell proliferation,invasive and migratory abilities,and the expressions of MMP-2,MMP-9,PI3K and p-Akt in the si-CCNB1 group were significantly lower than those in the si-NC group and control group(P＜0.05).There was no difference in the expression of Akt protein between the si-CCNB1 and si-NC groups(P＞0.05).Conclusion CCNB1 is highly expressed in oral squamous carcinoma cells.Silencing CCNB1 gene can inhibit the proliferation,invasion and migration of human oral squamous carcinoma SCC-15 cells.The mechanism may be related to the inhibitory activation of PI3K/Akt signaling pathway.

Key words: CCNB1 gene, PI3K/Akt signaling pathway, Oral squamous cell carcinoma, Proliferation, Invasion, Migration

CLC Number:

R739.8

WANG Pu, WEI Libin, NI Guangxiao. Effect of CCNB1 gene on proliferation,invasion and migration of oral squamous cell carcinoma cells by regulating PI3K/Akt signaling pathway[J]. Journal of Practical Oncology, 2020, 34(2): 144-149.

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URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2020.02.011

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2020/V34/I2/144

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Effect of CCNB1 gene on proliferation,invasion and migration of oral squamous cell carcinoma cells by regulating PI3K/Akt signaling pathway

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