细胞分裂周期7蛋白在肝癌组织的表达及其对肝癌细胞增殖和侵袭的影响

doi:10.11904/j.issn.1002-3070.2020.02.012

Abstract

Abstract: Objective The objectives of this study were to investigate the expression of cell division cycle 7 protein(CDC7)in hepatocellular carcinoma(HCC)tissues,and to explore the effect of CDC7 overexpressing on proliferation and invasion of hepatocellular carcinoma cells.Methods Quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the expression of CDC7 mRNA in liver cancer tissues and adjacent tissues,and the expression of CDC7 mRNA in liver tumor cell lines and normal liver cells.Western blot was used to detect the expression of CDC7 protein in liver cancer tissues and adjacent tissues,and to detect the expression of CDC7 protein in liver tumor cell lines and normal liver cells.Lentivirus vectors were used to construct stable overexpressing CDC7 hepatocellular carcinoma HCCLM3 and SMMC 7721 cell lines.Cell clone and cell counting kit-8(CCK-8)experiments were used to detect the effect of CDC7 overexpression on the proliferation of HCC cells.Scratch and Transwell assays were used to detect the effect of CDC7 overexpression on the invasion and migration of HCC cells.Results Compared with adjacent tissues,the expression of CDC7 at the levels of mRNA and protein was significantly increased in HCC tissues(P＜0.001);compared with normal liver cells,the expression levels of CDC7 at the levels of mRNA and protein were significantly increased in liver cancer cell lines(P＜0.001);CCK-8 experiments and cell cloning experiments showed that overexpression CDC7 significantly enhanced the proliferative ability of HCC cells;Scratch and Transwell experiments showed that overexpression CDC7 significantly improved the invasion ability of HCC cells.Conclusion CDC7 protein is highly expressed in human HCC tissues,and its overexpression promotes the proliferative and invasive capability of HCC cells.

Key words: Hepatocellular carcinoma, Cell division cycle 7 protein, Proliferative, Invasion

CLC Number:

R735.7

ZHANG Congyi, CUI Yifeng, LI Baobao, LIU Lianxin. Expression of cell division cycle 7 protein in hepatocellular carcinoma tissues and its effect on proliferation and invasion of hepatocellular carcinoma cells[J]. Journal of Practical Oncology, 2020, 34(2): 150-155.

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URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2020.02.012

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2020/V34/I2/150

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Expression of cell division cycle 7 protein in hepatocellular carcinoma tissues and its effect on proliferation and invasion of hepatocellular carcinoma cells

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