Abstract: Objective To investigate the expression and clinical significance of ras-p21, p53 gene protein in inguinal lymph nodes with penis carcinoma.Methods The clinical data of 44 cases of perfect penis carcinoma from 1990 to 2002, selected 44 of inguinal lymph nodes of them at random, pathological examination showed that 18 cases had metastatic carcinoma, 26 were inflammatory affection.Separately, selected 20 of nonspecific inflammatory inguinal lymph nodes synchronously, 20 of normal lymph nodes, paraffin-embedded, sectioned, SP stained, examined the expression of ras-p21, p53 gene protein.Results The expression of ras-p21, p53 gene protein in the group of inguinal lymph nodes with penis carcinoma is significantly higher than those in normal lymph nodes and nonspecific inflammatory lymph nodes, it is positive correlation with tumor clinical stage(P＜0.05).Cancerometastasis of inguinal lymph nodes is significant different compared with the control group(P＜0.05).Conclusions It can improve the diagnosis rate to detect ras-p21, p53 gene protein at the same time of lymph nodes biopsy.The detection of ras-p21 and p53 gene protein can be the Objective target to criterion metastasis and prognosis.And so, it can be the decision index whether the elimination of inguinal lymph nodes is necessary.