Abstract: Objective To investigate the influence of Wtp53 gene on cytochondriome function of fibrosarcoma in mouse.Methods Ninety Kunming mice(5weeks)were randomly divided into two groups, control group and Wtp53-therapy group.Kunming mice were subcutaneously injected with S180 fibrosarcoma suspension liguor(0.1mL)at femur of posterior limb.Five days after first injection, the mice were injected with sterile saline water(0.1mL)and Wtp53 adenovirus liguor respectively at the first-injection area.Two groups of mice were separately killed at 10d, 20d and 30d after the second injection.Drawing out of the cytochondriome with Eseabroob method, respiration of cytochondriome was measured using the method of Clark, Ca²⁺ with Reer method.Results Apoptosis index in control group was lower than that in therapy group.R₃ in control group was significantly higher than that in therapy group;R₄ of the two groups were closely in 10 days while extended in 20 or 30 days in therapy group, and there existed significantly difference;RCR in control group had not been found obvious change;while in therapy group RCR decreased sharply than that in therapy group, free concentration of Ca²⁺was similar during the 10 days and then increased clearly.Conclusion Wtp53 can obviously reduce the respiration of cytochondriome, increased the penetration of cytochondriome and suppress tumor through reducing proliferation and inducing apoptosis of tumor cells.