Objective To investigate the correlation of EGFR, C-erbB-2 and VEGF expression in gastric carcinoma and normal gastric tissues and their significances to clinicopathological character.Methods one hundred and twenty cases of gastric carcinoma specimens were selected with full clinicopathological data, and another thirty cases of normal gastric mucosa specimens were selected as the control.SP immunohistochemistry was used to measure the expression of EGFR, C-erbB-2 and VEGF.The data were statistically analyzed by χ² test and kappa test.Results The positive rates of EGFR, C-erbB-2 and VEGF in gastric carcinoma(40.00%、26.70% and 52.50% respectively)were significantly higher than those in the normal tissue(7.14%、3.43% and 7.14% respectively).EGFR, C-erbB-2 and VEGF expression was not correlated with age, sex, size and degree of differentiation(P＞0.05);the positive expression rates of EGFR, C-erbB-2 and VEGF were related to depth of invasion, lymph nodes metastasis, clinical stage and distant metastasis(P＜0.05).There are significantly positive correctionship among EGFR, C-erbB-2 and VEGF expression in gastric cancer(Kappa＞0.4, P＜0.05).Conclusion The Results suggest that the high expression of EGFR, c-erbB-2 and VEGF is an useful tool for providing information about the malignant degree, prognosis, and may be as a guiding in targeted treatments for patients with gastric carcinoma.

Objective To investigate the effects of the anti-clotting protein component from venom of Agkistrodon inducing SW480 apoptosis and its mechanism.Methods CCK-8 method was used to detect the antitumor effect of CTX-d in vitro;flow cytometry were used to observe the apoptotic and cell cycle inducing effect of the anti-clotting protein component from venom of Agkistrodon in SW480 cells.Mitochondrial transmembrane potential change(Arpm)was analyzed by flow cytometry;The levels of caspase-3 and bcl-2 were analyzed by Western blotting.Results The generation depressant effects of SW480 cells cultured in vitro were detected by CCK-8 method(P＜0.05), and there were dosage-time dependent relationships.Typical changes of apoptosis such as the suspending cells and the cellular debris got more, the process of cell division got less were observed, and subdiploid peak can be detected.Caspase-3 expression enhanced but bcl-2 expression did not changed obviously with the anti-clotting protein component from venom of Agkistrodon's concentration increase.Conclusions The anti-clotting protein component from venom of Agkistrodon can induce apoptosis in SW480, the mitochondria／caspase-3-specific pathway may be involved in the mechanism.

Objective To investigate the expression and clinical significance of ras-p21, p53 gene protein in inguinal lymph nodes with penis carcinoma.Methods The clinical data of 44 cases of perfect penis carcinoma from 1990 to 2002, selected 44 of inguinal lymph nodes of them at random, pathological examination showed that 18 cases had metastatic carcinoma, 26 were inflammatory affection.Separately, selected 20 of nonspecific inflammatory inguinal lymph nodes synchronously, 20 of normal lymph nodes, paraffin-embedded, sectioned, SP stained, examined the expression of ras-p21, p53 gene protein.Results The expression of ras-p21, p53 gene protein in the group of inguinal lymph nodes with penis carcinoma is significantly higher than those in normal lymph nodes and nonspecific inflammatory lymph nodes, it is positive correlation with tumor clinical stage(P＜0.05).Cancerometastasis of inguinal lymph nodes is significant different compared with the control group(P＜0.05).Conclusions It can improve the diagnosis rate to detect ras-p21, p53 gene protein at the same time of lymph nodes biopsy.The detection of ras-p21 and p53 gene protein can be the Objective target to criterion metastasis and prognosis.And so, it can be the decision index whether the elimination of inguinal lymph nodes is necessary.

Objective To investigate the influence of Wtp53 gene on cytochondriome function of fibrosarcoma in mouse.Methods Ninety Kunming mice(5weeks)were randomly divided into two groups, control group and Wtp53-therapy group.Kunming mice were subcutaneously injected with S180 fibrosarcoma suspension liguor(0.1mL)at femur of posterior limb.Five days after first injection, the mice were injected with sterile saline water(0.1mL)and Wtp53 adenovirus liguor respectively at the first-injection area.Two groups of mice were separately killed at 10d, 20d and 30d after the second injection.Drawing out of the cytochondriome with Eseabroob method, respiration of cytochondriome was measured using the method of Clark, Ca²⁺ with Reer method.Results Apoptosis index in control group was lower than that in therapy group.R₃ in control group was significantly higher than that in therapy group;R₄ of the two groups were closely in 10 days while extended in 20 or 30 days in therapy group, and there existed significantly difference;RCR in control group had not been found obvious change;while in therapy group RCR decreased sharply than that in therapy group, free concentration of Ca²⁺was similar during the 10 days and then increased clearly.Conclusion Wtp53 can obviously reduce the respiration of cytochondriome, increased the penetration of cytochondriome and suppress tumor through reducing proliferation and inducing apoptosis of tumor cells.

Objective To investigate the mechanism of transforming growth factor(TGF)-β1 on metastasis in human ovarian cancer cells.Methods Using RT-PCR, Western Blot and Zymography, we determined the effects of TGF-β1 on the expressions and secretions of MMP-2 and MMP-9 in HO-8910 and HO-8910PM cells.In addition, expressions of TGF-β1, MMP-2 and MMP-9 were determined by immunohistochemistry in normal ovary tissues(31 cases), primary ovarian cancer tissues(45 cases)and metastatic tissues(45 cases).Results We found that the expressions and secretions of MMP-2 and MMP-9 were enhanced by TGF-β1 in HO-8910 cell.There was significant difference regarding the expression of MMP-2 between normal ovarian tissues and primary ovarian cancer tissues(P＜0.05).Significant difference of the expression of TGF-β1, MMP-2 and MMP-9 was also found between primary ovarian cancer tissues and metastatic tissues(P＜0.01).There were correlations between the expression of TGF-β1 and MMP-2(P＜0.05)and between the expression of TGF-β1 and MMP-9(P＜0.05).Conclusions In ovarian cancer, TGF-β1 promotes metastasis by increasing the expression of MMP-2 and MMP-9.

Objective To discover the inhibitory effect of Doxycycline on invasion of Human colorectal carcinoma LS174T cells in vitro.Methods Artificial recombinant basement membrane invasion assay was utilized through Transwell model.Zymography was used to observe the decrease of MMP2.Results Doxycycline could inhibit the invasion of human colorectal carcinoma LS174T cells in vitro.With 5mg/L, 10mg/L and 20mg/L Doxycycline, the number of cells which could invaded through the Matrigel was respectively 101.3±6.7, 83.7±5.7 and 42.0±2.0.The inhibition ratio was respectively 17.9%, 32.0% and 65.9%, dose-dependently.Under 5mg/L, Doxycycline could decrease the secretion of MMP-2.Conclusion Doxycycline had inhibitory effect on invasion of colorectal carcinoma LS174T cells through inhibition of the secretion MMP-2 under a low dose.Doxycycline was a potential agent inhibiting tumor invasion and metastasis.

Objective To evaluate the molecule mechanisms efficacy of two neoadjuvant chemotherapys(NACT)on locally advanced cervix cancer(LACC), and according to this for selecting treatment way.Methods 40 patients, which had been divided into two groups, were treated with two cycles of cisplatin combined with ifosfomide or cisplatin combined with paclitaxol, meanwhile, Ki-67、apoptosis index(AI)and survivin were detected and assayed with flow cytometry and TUNEL.Results The clinical efficacy rates of the two groups were 75%(IP group)and 95%(TP group)respectively, there was statistical difference(P＜0.05).Significant difference of Ki-67、survivin and AI could be observed before and after NACT(P＜0.05).Conclusion Two kinds of NACT could inhibit proliferation and increase apoptosis of tumor cells in cervical squamous carcinoma tissue.The clinical efficacy and molecule treatment efficacy of the group IP is equal to the group TP, but the latter need a shorter time of treating and in the hospital, so the patients are easy to take it and we should be encouraged to adopt it.

Objective To explore the mechanism of multi-drug resistance(MDR)in the human gastric carcinoma MDR subline SGC7901/VCR.Methods The density of vincristine(VCR)was increased gradually to induce the drug resistance of gastric carcinoma cell SGC7901.MTT assay was used to determine the lethal effect of anticarcinogens on tumor cells and Western blot assay was applied to determine the expression of P-gp and GST-s in tumor cells.Results The resistance of SGC7901/VCR cells to VCR, fluorouacil, and epirubicin were 16.56, 2.69 and 13.05 times, respectively, more than that of SGC7901 cells.Expressions of P-gp and GST-s in resting SGC7901/VCR cells were significantly higher than that in carcinogen-sensitive SGC7901 cells.Conclusion The density of vincristine(VCR)was increased gradually to induce the drug resistance of gastric carcinoma cell SGC7901.Expression of P-gp and GST-s in resting SGC7901/VCR cells was significantly more than that in carcinogen-sensitive SGC7901 cells.On the contrary, Inhibiton of P-gp and GST-s may reverse drug resistance of SGC7901/VCR cells.

Objective To investigate the pathological features and immunophenotype which were related to recurrence of the uterine adenosarcoma in adolescence.Methods The primary and recurrent uterine adenosarcoma in an adolescence were observed by morphology and immunohistochemistry.The related literatures were reviewed.Results The primary and recurrent tumor both were composed of an admixture of benign-appearing glands and a sarcomatous stroma.The sarcomatous stroma in primary tumor constructed by endometrial stromal sarcoma with sarcomatous overgrowth and had myometrial invasion.Recurrent tumor possessed more sarcomatous area and had chondrosarcoma compared with primary tumor.Immunohistochemically, the sarcomatous stroma cells in primary tumor:p53(+), Ki-67(＞70%, +), ER(-), PR(-).Conclusion The pathological features and immunophenotype related to recurrence may be sarcomatous overgrowth, myometrial invasion, immunohistochemical stain positive for p53, strongly positive for Ki-67 and negative for ER, PR.

Objective To study the role of PKC in multidrug resistance of bladder carcinoma.Methods Takai method was followed to measure the activity of PKC in bladder tumor tissues and normal tissues around the tumor.And RT-PCR method was used to measure the mRNA expression of P-gp and MRP in both region as PKC.Results Compared with that of the normal tissues around the tumor in the different tumor stages and grades, PKC activity in carcinoma increased significantly in plasma and membranous;with the increasing of tumor stage and grade, PKC activity increased in the tumor tissues.P-gp expression in bladder tumor tissues and normal tissues around the tumor were 90%, 100% respectively, bladder tumor tissues was lower than that of normal tissues significantly.And MRP expressed in all these tissues, bladder tumor tissues was higher than that of normal tissues significantly.P-gp had not difference in both grades and stages, and MRP increased with grades significantly, but not with stages.In addition, MRP was correlative with PKC activity, P-gp wasn’t correlative with PKC activity.Conclusion Overexpression of PKC in the bladder carcinoma tissues might contribute to the bladder multidrug resistance of bladder carcinoma, PKC might play an important role in the multidrug resistance of bladder carcinoma.

Objective To evaluate dose volume history in process of lung cancer radiotherapy using image fusion technique completely.Methods 25 patients who did not undergo operations, median age 54 and Karnofsky Performance Status≥70.All received three-dimensional conformal radiation therapy using CT-sim, and were scanned by CT-sim repeatedly after 40-50Gy radiotherapy.We made image fusion of the images before and after radiotherapy using ACQsim working station and overlayed total dose of normal tissues through radiotherapy planning system.Results Radiotherapy was finished for every patient and total dose was 60-66Gy.DVH could reflect the suffering dose of normal pulmonary tissue, spinal cord and heart completely.Conclusion Adjustment of total dose during radiotherapy period by the suffering dose of normal tissues could decrease complication occurrence in process of radiotherapy.

Objective To evaluate the efficacy and adverse side effects of controlled-release oxycondone in the management of moderate to severe cancer pain in patients with teminal cancer and to observe any improvement on the life quality of cancer patients.Methods Thirty-eight patients with moderate or severe cancer pain were treated with controlled-release oxycondone.The initial dose was 5mg q12h to the cancer patients with moderate pain or 10mg q12h to the cancer patients with severe pain, or 1/2 that of the standard morphine regimen.Results The final titrated dosage of oxycondone was as follows:the patients in 17 cases(44.7%)received a dosage of ≤30mg/d, those in 9 cases(23.7%)received a dosage of 31 to 60mg/d, those in 10 cases(26.3%)received a dosage of 61 to 120mg/d and those in 2 cases(5.3%)received a dosage of ≥120mg/d.The overall rate of relief from pain was 94.7%.The common side effects reported were constipation in 40 cases(65.6%), dizziness in 9 cases(14.7%), nausea in 7 cases(11.5%)and somnolence in 5 cases(8.2%)during controlled-release oxycodone treatment.No patient had respiratory depression.Conclusion The side effects of controlled-release oxycodone are mild, and the drug is effective and safe for the management of cancer patients with moderate to severe pain and can significantly improve the life quality of cancer patients with pain.

Objective To study the expression of p53、p21WAF-1 and p63 in gastrointestinal stromal tumors(GIST)and the relationship with clinicpathological factors.Methods The expression level of p53、p21WAF-1 and p63 in 70 cases of GIST was examined by using SP immunohistochemical method.Results The positive expression rate of p53、p21WAF-1 and p63 was 62.86%、34.29% and 14.29% respectively.The positive expression of p53 was related to primary location of tumor, necrosis, tumor diameter, nuclear mitosis and Fletcher grading(P＜0.05).The positive expression of p21WAF-1 was related to tumor cell types, tumor diameter, nuclear mitosis and Fletcher grading(P＜0.05).The positive expression of p63 was related to necrosis and nuclear mitosis(P＜0.05).The expression of p21WAF-1 showed negative correlation with that of p53.Conclusion The expression level of p53、p21WAF-1 and p63 was closely related with the biological behavior of GIST and might be considered as potential marker for the biological behavior of GIST.

Objective To evaluate the efficacy and safety of irinotecan(CPT-11)combined capecitabine for patients with advanced or metastatic colorectal cancer.Methods Sixty-two patients with histologically proven advanced colorectal cancer received irinotecan 125mg/m² on day 1, 8, 15 and capecitabine 2500mg/(m²·d)as an intermitent regimen on day 1 to 14.The regimen was repeated every 4 weeks and efficacy and toxicity were evaluated after 2 cycles.The efficacy and safety were assessed on the basis of WHO evaluation standard of Objective therapeutic effect for solid tumor.Results Fifty-nine patients were assessable for responses, including twenty-eight patients received the regimen as the first line chemotherapy and thirty-one patients as the second line chemotherapy.RR was 42.8%, DCR was 71.4%, TTP were 8.6 months and MST were 18.8 months in the first line chemotherapy.RR was 32.3%, DCR was 61.2%, TTP were 7.2 months and MST were 13.2 months in the second line chemotherapy.Dose limiting toxicity was delayed diarrhea and neutropenia.Most of them wereⅠ/Ⅱdegree and tolerable.There was no death during the treatment.Conclusion The combination chemotherapy of irinotecan with capecitabine is an active and tolerable regimen for advanced colorectal cancer.

Objective To evaluate the effect and toxicity of three-dimensional conformal radiotherapy(3DCRT)on non-small cell lung cancer(NSCLC)in elderly.Methods 58 patients with non-small cell lung cancer were radiated by 3DCRT, DT 2.0Gy/T, 5T/W, the overall DT64-70Gy;CT examination was performed in the 1 to 3 months followed-up period to verify the therapeutic effects.Results Among 58 patients, 17 patients were CR, 33 patients PR, 6 patients SD, 2 patients PD.Total effective rate(CR+PR)was 86.2%, serious toxicity was found to be the radiation esophagitis and radiation pulmonitis, but tolerable.Conclusions 3DCRT was an effective and tolerable regimen for treatment of elderly non-small cell lung cancer.

Objective To investigate the therapeutic effect, long term survival and toxicities for advanced NSCLC patients treated with domestic nadaplatin combined with docetaxel.Methods 57 patients with histologically and/or cytologically confirmed advanced NSCLC were treated with domestic nadaplatin 80-100mg/m² and docetaxel 75mg/m² on d1, repeated every 3 weeks.Results The response rate of all the patients were evaluated.There were 4CR, 16 PR, 24 SD and 13 PD in the group, CR add PR was 35.1%, CR add PR and SD was 77.2%.Median time to progression(TTP)was 4.3 months.Median survival duration was 7.4 months.One year survival rate was 34.2%.There was high response in hematological toxicity, non-hematological toxicity were gastrointestinal reactions.Conclusions Domestic nadaplatin combined with docetaxel in second-line treatment for advanced non-small-cell lung cancer is effective, and toxicity is tolerated.