Objective Intrinsic and/or acquired resistance was the major obstacle to overcome in the treatment of patients with breast cancer.The aim of the present study was to investigate the relationship between the expression of breast cancer resistance protein(BCRP)with the clinicopathologic characteristics and the prognosis in primary breast cancer patients.Methods Expression of BCRP in 47 breast cancer patients was determined by immunohistochemistry on formalin-fixed, paraffin-embedded tumor section.Results (1)BCRP expression was observed in 26 of 47(55.3%).(2)No relation can be established between expression of BCRP and other clinicopathologic parameters.(3)Kaplan-Meier analyses revealed that BCRP expression only associated with disease-free survival(DFS)and didn′t with overall survival(OS).(4)In one-factor Cox analysis, tumor size and BCRP-positive staining was associated with DFS(P＜0.05).In addition, tumor size also associated with OS(P＜0.05).In multivariate Cox regression analysis, BCRP expression only associated with DFS.Lymph node metastasis was independent prognostic factor for DFS and OS(P＜0.05).Conclusions Our Results suggested that BCRP is frequently expression in primary breast cancer, but BCRP is not a poor prognostic factor in patients with breast cancer.

Objective To investigate the role of expression of T cell costimulatory molecule ICOS and variance of T cell subpopulations in the development and prognosis of gastric cancer.Methods The peripheral blood lymphocytes were tested for T cell subpopulations and T cell costimulatory molecule ICOS by flow cytometry in 38 patients with gastric cancer and 21 healthy peoples as control group.Results Compared gastric cancer group with control group:expressions of T cell costimulatory molecule ICOS were(25.80±10.56)vs(0.82±0.98)%, P＜0.01;expression percentage of total T cell(CD3⁺)were(53.61±13.84)vs(72.07±7.83)%, P＜0.01;expression percentage of CD4⁺T cell(CD4⁺CD3⁺)were(29.84±9.71)vs(38.79±5.08)%, P＜0.01;expression percentage of CTL cell(CD8⁺ICOS⁺CD3⁺)were(1.57±1.99)vs(0.02±0.04)%, P＜0.01;expression percentage of CD8⁺inhibitory T cell(CD8⁺ICOS^-CD3⁺)ratio were(16.6±6.94)vs(20.56±6.54)%, P＜0.05.No difference of expression was found in peripheral T cell subpopulations of postoperative patients with gastric cancer after one week(P＞0.05).Conclusion T cell number decreased, and T cell costimulatory molecule ICOS expression incrensed in patients with gastric cancer.CD4+T cell subpopulation is significantly decreased in patients with gastric cancer.

Objective To search a good way to store CIK cells.Method Freeze CIK cells when they were induced for 14 days and anabiosis them after two months later.The anabiosis cells growth curve was drawn from the first day they recovered and the unfreezing cells growth curve was drawn after they induced for 14 days.The cytotoxic activities were determined by MTT and the cell phenotypes were detected by flow cytometry after anabiosis three days.Three indexes of anabiosis CIK cells were compared to the unfreezed.Results The anabiotic cells were in good condition.Compared with unfreezing CIK cells, the growth rate of freezed cells was lower, significant differences existed between the two groups(P＜0.05).Cytotoxic activities of CIK cells and the freezed ones were(51.1±2.0)%, (50.9±3.3)%, (36.4±3.2)% and(49.7±1.5)%, (34.7±1.3)%, (29.5±7.6)% according to the different effect and target cell ratios of 40∶1, 20∶1 and 10∶1.No significant difference existed between the two groups.The cell phenotypes CD3+CD56+ were(19.1±0.4)%, (19.5±0.8)%and there were no significant difference between the two groups.Conclusion Freezing had effect on the growth rate of CIK cells but had no influence on cytotoxic activity and cell phenotype.The method is available for clinical application.

Objective To compare the adverse effects of nedaplatin plus 5-Fu and cisplatin plus 5-Fu combined with synchronous radiotherapy on esophageal cancer.Methods From December 2007 to March 2009, 52 cases with esophageal carcinoma were randomly divided into two groups:concurrent chemoradiotherapy with NDP and 5-Fu(the NF group, n=24)and synchronous chemoradiotherapy with DDP and 5-Fu(the DF group, n=28).The patients in these two groups were treated with conventional fractionated radiotherapy at 2.0Gy/f, the postoperative with a total dose of 50Gy/25Fx/35d and the unoperated with a total dose of 64Gy/32Fx/44d.Chemotherapy regime was delivered on the first day of radiotherapy.The chemotherapy in the NF group consisted of 2 cycles of NDP at 60mg/(m²·d)on day 1 to 2 plus 5-Fu at 500mg(m²·d)on day l to 5 every 4weeks, and the DF group consisted of 2cycles of DDP at 25mg(m²·d)on day l to 4 plus 5-Fu at 500mg(m²·d)on day l to 5 every 4weeks.Results The incidence of major adverse effects in the NF group such as nausea, vomiting and response rate of nephrotoxicity were lower compared to that in the DF group(P＜0.05).There was no significant difference between two groups in the incidence of leukopenia(P＞0.05).There was a higher thrombocytopenia response rate in NF group than that in DF group, with a significant difference between the groups(P＜0.05).Conclusions NDP+5-Fu combined with radiotherapy can safely treat esophageal cancer and side effects is tolerant, so it is fit to the olds especially.However, the efficacy of the two groups is needed for further study.

Objective To retrospectively analyze the factors of polypus cancerization in large intestine, and to discuss the importance of total colonoscopy for colorectal cancer and colorectal polypus patients.Methods The endoscopic and pathologic features of colorectal cancer and colorectal polypus patients with bowel polyps detected by video colonoscope were retrospectively reviewed and analyzed.Results Of these 1100 cases, 1543 polyps were found in 387 patients.42 cases were found to have cancerization.Villous adenomatous polyps in the left colon, ≥2.0cm in diameter are liable to cancerization.Most colorectal cancers originated from the rectum or simgmoid colon, The broader the pedicles and the larger the diameters of polyps are, the higher the malignant rates could be.Of these 1100 cases, 326 colorectal cancers(29.63%)were detected.The age tend to suffer from colorectal cancer and colorectal polypus patients、polypus cancaration are similar higher than 45.92 cases of colorectal cancers were found to have concurrent colorectal polyps.Multiple primary colorectal carcinoma was observed in 29 cases.66 patients who have had colorectal cancer operation were detected to have colorectal polyps.Conclusion Many factors, such as patient′s age, the size, morphology, pathology pattern and location of the polyp, were related to colorectal cancerization.Total colonoscopy helps diagnose colorectal cancer, reduce the rate of misdiagnosis and improve the survival.

Objective To study the effect of hepatocyte transplantation(HCT)intraperitoneal in rats with acute liver failure(ALF)which were induced by D-galactosamineN, and to discuss whether this is an effective way.Methods ALF animals were induced by D-GalN.HCT was received 48h after intoxication.GroupⅠreceived 2.5×10⁷hepatocytes intraperitoneally.GroupⅡreceived 2.5mL NS intraperitoneally.The survival rates, liver function, liver histology and biologic characteristics of transplanted cells were assessed.Survival rate of groupⅠwas much higher than that of groupⅡ(54.2% vs 8.3%, P＜0.005), the liver function and liver histology were partially improved in groupⅠas compared with groupⅡ.Conclusion Survival rate of ALF rats intoxicated with D-GalN could be improved, the liver function and liver histology could also be partially improved, via intraperitoneal HCT.

Objective To select the CT characteristics and clinicalopathological parameters of non-small lung cancer which can reflect the expression of Ki-67、survivin and cyclinA protein.Method 33 cases of lung cancer(squamous carcinoma 11 cases and adenocarcinoma 22 cases)have undergone the scanning of 18F-FDG PET/CT before operation.The relationships between CT features, pathological classification, tumor differentiated types, lymph node metastasis and the expression of Ki-67、survivin and cyclinA which were examined by means of SP immunohistochemical technique.Results The positive rates of Ki-67、survivin and cyclinA protein were 69.70%、84.85% and 45.45% respectively.Significant differences in the positive rate of Ki-67 and cyclinA were found between squamous carcinoma and adenocarcinoma(P=0.031 and P=0.032)and between the size ≥2cm and＜2cm(P=0.025), but not among differentiated types, lymph node metastasis and CT feature of lobulation, thin and short spiculation, spinous protuberance, cavity sign, vessel convergence sign and pleural retraction sign(P＞0.05).The positive rate of cyclinA was also related to vessel convergence sign and pleural retraction sign(P=0.027, P=0.032), but not related to other data(P＞0.05).There were no correlations between the positive rate of survivin and other data.Conclusion The study suggested that the ability of proliferation and the speed of cell cycle of squamous carcinoma were stronger than that of adenocarcinoma.The size of tumor can reflect the stronger ability of proliferation.The pleural retraction sign can reflect the faster cell cycles.

Objective To study the therapeutic effects of clarithromycin, amoxicillin and omeprazole tripal therapy for eradication of Helicobacter Pylori(H.pylori)combined with chemotherapy on patients with HP positive advanced gastric carcinoma. Methods 42 patients were all HP positive advanced gastric carcinoma who were divided into two groups.Patients in the control group were treated with FolFox regiment once three weeks.One course of treatment included 6 cycles.All patients were treated with one course at least.Patients in the treatment group were treated with Folfox regiment as well as omeprazole, clarithromycin and amoxicillin given orally.Observe the curative effect, toxic reaction and survival time of the two group after the treatment.Results The number of CR, PR, SD, PD was 4, 9, 5 and 4 respectively in the treatment group.The total effective rate was 59.09%, while the corresponding number in the control group was 1, 5, 6, 8 and 30.0%.The curative effect of the two group had statistical difference while the side and toxic effect of the two group was not different statistically.Conclusion Clarithromycin, amoxicillin and omeprazole tripal therapy for eradication of Helicobacter Pylori(H.pylori)combined with chemotherapy has higher effective rate, similar side-effect rate compared with the traditional chemotherapy.It has certain significance in the treatment of advanced gastric carcinoma, though the two year survival rate was not obvious different in the two group.

Objective To investigate the expression and clinical significance of Ki-67 and p53 in breast invasive ductal carcinoma(IDC).Methods Immunohisto chemical SP method was applied to detect the expression of Ki-67 and p53 protein in 42 cases with IDC and 18 cases with benign breast lesions, statistical analysis was performed to correlate the expression of Ki-67 and p53 with their clinical parameters.Results The positive expression percentage of Ki-67 and p53 was significantly higher in IDC cases than that in benign lesions cases(78.6% VS 0, 38.1 VS 5.6%).There was significant association between Ki-67, p53 expression and TNM staging, Ki-67 and p53 increasing correlated with higher TNM staging.There was another significant association between Ki-67 expression and tumor size.Conclusion The findings suggest that the positive expression of Ki-67 and p53in IDC make a closed correlation with TNM staging, Co-determining the expression of Ki-67 and p53in IDC case may play an important role in prediction of the IDC prognosis.

Objective To investigate the expression of TRADD and NF-κB in hepatocellular carcinoma and liver cirrhosis after HBV-infected and its clinical significance.Methods Western blot was used to detect the protein expression in 35 HCC and 35 LC cases.The Results were statistically analyzed by Fisher′s exact test.Results Expression of TRADD was found in 24 of 35 cases in HCC and all of 35 cases in LC(P＝0.0002).Expression of NF-κB was found in 27 of 35 cases in HCC and all of 35 cases in LC(P=0.002).Expression of TRADD was correlated with the lever of the α-fetoprotein in HCC.Conclusion TRADD and NF-κB might mainly be activated in LC with a long-term HBV infection.The abnormal pathway may play important roles in HBV-induced hepatocyte transformation and carcinogenesis.

Object To discuss the relation of high risk HPV load and cervical lesions degree.Methods We tested HPV DNA of 2751 outpatients in our hospital, and performed biopsy to 971 patients with colposcope among them.Results There were not obvious difference among HPV load test value distribution of different degree cervical lesions.But there was remarkable relativity among the degrees of cervical lesions and HPV load, which was divided into low load dose and high load dose.Conclusion There are not distinct limit among virus load test value of different degree cervical lesions, and virus load can not represent the severity of cervical lesions.But the higher virus load can prompt the more possibility of high cervical lesions.

Objective To observe the Results of paclitaxel liposome and cisplatin in the treatment of recurrent ovarian cancer.Methods 82 cases of recurrent ovarian cancer were divided into 2 groups, experimental group(40 cases)and control group(42 cases), each experimental group was given paclitaxel liposome treatment 135mg/m², 1, 8-day intravenous infusion(intravenous);cisplatin 100mg, at the first intravenous infusion of 1-5 days;control group, 1500mg of fluorouracil and cisplatin 100mg, given in accordance with conventional doses.Epithelial ovarian cancer antigen(CA125)value, narrow focus, the survival rate, the sensitivity of different pathological types, as well as adverse reactions were observed.Results CA125 decline in the rate of 27 percent, the rate of lesion reducing was 32%, 1-year survival rate of 72%, in the control group were 10%, 10%and 35% respectively, the difference was significant(P ＜0.05).Clear cell carcinoma was more sensitive to paclitaxel liposome than that of serous carcinoma.The main adverse reactions of the experimental group were diarrhea, vomiting, neutropenia, intestinal obstruction and neurotoxicity.Conclusion Paclitaxel liposomes and cisplatin could be an effective treatment program for recurrent ovarian cancer.