Abstract: Objective To investigate the effects of the anti-clotting protein component from venom of Agkistrodon inducing SW480 apoptosis and its mechanism.Methods CCK-8 method was used to detect the antitumor effect of CTX-d in vitro;flow cytometry were used to observe the apoptotic and cell cycle inducing effect of the anti-clotting protein component from venom of Agkistrodon in SW480 cells.Mitochondrial transmembrane potential change(Arpm)was analyzed by flow cytometry;The levels of caspase-3 and bcl-2 were analyzed by Western blotting.Results The generation depressant effects of SW480 cells cultured in vitro were detected by CCK-8 method(P＜0.05), and there were dosage-time dependent relationships.Typical changes of apoptosis such as the suspending cells and the cellular debris got more, the process of cell division got less were observed, and subdiploid peak can be detected.Caspase-3 expression enhanced but bcl-2 expression did not changed obviously with the anti-clotting protein component from venom of Agkistrodon's concentration increase.Conclusions The anti-clotting protein component from venom of Agkistrodon can induce apoptosis in SW480, the mitochondria／caspase-3-specific pathway may be involved in the mechanism.