Abstract: Objective To investigate the expressions of Kiss-1 and E-cadherin in laryngeal squamous cell carcinoma, as well as to approach the role and correlation of Kiss-1 and E-cadherin.Methods Kiss-1 and E-cadherin expressions were detected in 78 laryngeal squamous cell carcinoma and 78 normal laryngeal tissue samples by immunohistochemical Method to analyze the correlation between the protein expression rate and clinical and pathological characters and study the correlation between the expression of Kiss-1 and E-cadherin in squamous cell carcinoma of the supraglottic larynx.Results Expression of every gene was significantly different between laryngeal squamous cell carcinoma and normal laryngeal tissues(P＜0.001).The negative expression rate of Kiss-1 and E-cadherin was significantly associated with cervical lymph node metastasis and clinical stage(P＜0.001), but not associated with sex and age of the patients(P＞0.05).The negative expression of Kiss-1 is closely related with the negative expression of E-cadherin(P＜0.001).Conclusion The expressions of Kiss-1 and E-cadherin in case and control group has a significant difference.Kiss-1 loss of expression as well as E-cadherin may be a biological maker in squamous cell carcinoma of the supraglottic larynx.The levels of Kiss-1 and E-cadherin were useful molecular markers for evaluating malignancy degree and lymph node metastasis of in laryngeal squamous cell carcinoma, and may play important roles in inhibiting the invasion and metastasis.