PRACTICAL ONCOLOGY JOURNAL ›› 2012, Vol. 26 ›› Issue (6): 501-505.doi: 10.3969/j.issn.1002-3070.2012.06.005

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The effect of PAK1 on migration and invasion of laryngeal cancer cells

HE Guoqing1,GUAN Qiang1,ZHANG Xijun2,ZHANG Jiewu1,JI Hongfei3   

  1. 1.Department of Head and Neck,The Third Affiliated Hospital of Harbin Medical University,Harbin 150081,China;
    2.Department of Otolaryngology,The Fourth Hospital of Harbin Medical University;
    3.The Institute of Cancer Prevention and Treatment of Harbin Medical University
  • Received:2012-04-06 Online:2012-12-28 Published:2014-12-03

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Abstract: Objective To investigate the expression of PAK1 in laryngeal cancer and its effect on migration and invasion of laryngeal cancer cells.Methods Firstly,22 samples of fresh laryngeal cancer tissues and adjacently normal laryngeal mucosa were collected to detect the expression of PAK1 by Western blot.Secondly,63 paraffin embedded laryngeal tissues were analyzed by immunohistochemistry to detect the expression of PAK1.Finally,We observed cell migration and invasion by knockdown of PAK1 in FaDu cell line via siRNA.Results The results from western blot and immunohistochemistry demonstrated that the expression of PAK1 in laryngeal cancer tissues was higher than that of normal mucosa.The cell migration and invasion of laryngeal squamous cell carcinoma was reduced after PAK1 was knocked down through siRNA.Conclusion The activation of PAK1 may contribute to the development and progression of laryngeal squamous cell carcinoma.Inhibition of PAK1 could decrease cell migration and invasion capability of laryngeal squamous cell carcinoma.

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