MicroRNA-204对胶质瘤细胞U251自噬的影响

doi:10.11904/j.issn.1002-3070.2016.01.005

Abstract

Abstract: Objective To investigate the effect of microRNA-204(miR-204)on autophagy in U251.Methods Inhibition of miR-204 in U251 cell lines was done by transfection of miR-204 inhibitor(AMO-204).Cell viability was detected by MTT assay.The autophagy of U251 was tested by immunofluorescence technique.The protein level of Beclin 1,LC3 and Bcl-2 was detected by Western blot.Results Cell viability was markedly increased after inhibition of miR-204 in U251 cells.The number of autophagosome was decreased.The levels of Beclin 1 and LC3 were decreased,the protein level of Bcl-2 was significant increased by transfection of AMO-204 in U251 cells(P＜0.05).Conclusion MiR-204 might at least in part promote glioma via inhibiton autophagy,indicating that miR-204 might be a potential target for the treatment of glioma.

Key words: Glioma, MicroRNA-204, Bcl-2, Autophagy

CLC Number:

R739.41

FU Shuang, LIU Guojun. The effect of microRNA-204 on autophagy in U251[J]. PRACTICAL ONCOLOGY JOURNAL, 2016, 30(1): 23-27.

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The effect of microRNA-204 on autophagy in U251

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