miR-219在舌鳞状细胞癌中的表达及意义研究

doi:10.11904/j.issn.1002-3070.2016.05.007

Abstract

Abstract: Objective To investigate the expression of miR-219 and its role in tongue squamous cell carcinoma(TSCC).Methods The relative expression of miR-219 in the 26 samples of surgically resected paired TSCC and normal tumor-adjacent tissues was detected by qRT-PCR.The expression of miR-219 in SCC-15 and normal oral mucosa cell were also tested by qRT-PCR.The miR-219 mimic was transferred into SCC-15 cell.Cell proliferative rate and the ability of colony formation were analyzed.The abilities of migration and invasion in SCC-15 cell were evaluated by Transwell test.Results Expression of miR-219 was significantly lower in TSCC tissues(P＜0.001)and SCC-15(P＜0.05)in comparison with normal tumor adjacent tissues and normal oral mucosa cell.The abilities of proliferation(P＜0.05),colony formation(P＜0.05),migrationand invasion(P＜0.05)were inhibited in miR-219-transferred SCC-15 cells.Conclusion The expression of miR-219 was downregulated in TSCC.The miR-219 could inhibit cell proliferation,colony formation,migration and invasion in TSCC.

Key words: Tongue squamous cell carcinoma, miR-219, Proliferation, Migration, Invasion

CLC Number:

R739.86

YUAN Zhennan, SONG Kaibin. Expression and significance of miR-219 in tongue squamous cell carcinoma[J]. PRACTICAL ONCOLOGY JOURNAL, 2016, 30(5): 420-424.

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Expression and significance of miR-219 in tongue squamous cell carcinoma

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