甘草提取物的有效部位诱导人宫颈癌HeLa细胞株凋亡及对凋亡相关蛋白Caspase-3和Caspase-9表达的影响

doi:10.11904/j.issn.1002-3070.2017.05.002

Abstract

Abstract: Objective The aim of this study was to determine whether active part of Glycyrrhiza extract(GL)induced apoptosis in Hela cells and its inhibitory mechanism.Methods HeLa cells were treated with 25 g/mL of GL for 24hs.Cell viability and apoptosis in HeLa cells were determined by MTT,AO/EB fluorescent double staining,transmission electron microscope(TEM),and Western blot.Results The MTT results showed that GL significantly inhibited the proliferation of HeLa cells in a dose-response.After treatment for 24 hrs,large number of early apoptotitc cell were observed using AO/EB fluorescent double staining and TEM.The expression of Pro-caspase-9 and Cleaved-caspase-3 protein was higher in GL-treated cells them those of the control cells(P＜0.05).Conclusion GL can activate Caspase-3 and Caspase-9 genes to induce apoptosis in HeLa cells.

Key words: Licorice, Anti-tumor effect, Apoptosis, HeLa cells, Apoptosis protein

CLC Number:

R737.33

WANG Jixi, DENG Weisheng, WANG Fengru, SHI Zhimao, MENG Dan, XU Qiang, ZHANG Yuyao. Active part of Glycyrrhiza extract induces apoptosis in human cervical cancer HeLa cells and its related protein caspase-3 and caspase-9 expression[J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(5): 390-395.

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Active part of Glycyrrhiza extract induces apoptosis in human cervical cancer HeLa cells and its related protein caspase-3 and caspase-9 expression

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