Objective To evaluate the influence to stage and the treatment planning in non-small cell lung cancer(NSCLC)by CT、PET/CT.To define the correlation of gross tumor volume(GTV)by PET/CT and pathology.Methods From March 2006 to July 2008,37 NSCLC patients were studied,21 cases received surgical resection and 16 cases received radiotherapy based on PET/CT.All patients had PET/CT scans of all body before surgery or radiotherapy and routine pathology examination after surgery.The tumor size at X(lateral direction)、Y(ventrodorsal direction)and Z(craniocaudal direction)axes were measured on CT、PET/CT and pathology examination,respectively.TNM stage change the treatment planning in a significant number of patients,both the CT and PET/CT images were transferred to the RT planning workstation for contouring.GTV,pathologic nodal and primary tumor volumes were first defined in the conventional manner based on CT and PET/CT images.Results TNM stage was changed in 19 cases(51.4%)and the treatment planning was changed in 12 cases(37.8%)after PET/CT.There was no significant difference in the tumor size at X 、Y axes(P＞0.05);and there was significant difference in the tumor size at Z axes.Of the 16 patients in radiotherapy group,the primary tumor GTV was increased in 4 cases by detecting additional nodal disease burden,and decreased GTV in 7 cases by differentiating atelectasis and postobstructive pneumonia from tumor.Conclusion PET/CT alter TNM stage and the treatment planning in NSCLC in a significant number of patients,there was almost total agreement on the gross tumor volume by PET/CT and pathology.GTV could be used for radiation therapy by PET/CT instead of the GTV by pathology.PET/CT is superior CT on delineation of the gross tumor volume for radiation planning.

Objective To observe the effect of ginsenoside Rg3(Shenyi capsule)on serum level of VEGF and treatment efficacy on NSCLC patients.Methods NSCLC patients who were in ⅢB and could not conduct operations were selected.In experiment group,patients have been treated by chemotherapy combined with ginsenoside Rg3 taken orally,20mg per time,twice a day and continuously for 2 courses of treatment.In control group,patients have been treated only by chemotherapy.After treatment were completed,patients serum levels of VEGF,CEA,quality of life,toxicity and adverse effect were observed.Results After treatment were completed,compared with control group,serum levels of VEGF and CEA decreased obviously(P＜0.05)in experiment group and patients quality of life were improved obviously(P＜0.05).The incidence of toxin and side effect in experiment group was obviously lower.Conclusion NSCLC patients who were in ⅢB and could not conduct operations accepting chemotherapy combined with ginsenoside Rg3 can inhibit tumor-related angiogenesis,increase the anti-tumor efficacy and improve the patients quality of life.

Objective The aim was to investigate the relationship between the positive rate of exfoliative cancer cells of gastric cancer and clinicopathological factors.Methods We reviewed the records of 100 patients diagnosed with gastric carcinoma who were resected surgically.In order to test the positive rate of peritoneal free cancer cells,peritoneal washes(500mL)were obtained after laparotomy.Then we investigated the relationship between positive rate of peritoneal free cancer cells of gastric cancer and clinicopathological features.Results Free cancer cells of peritoneal washes were in 20 patients,the positive rate was 20%.Univariate analysis showed that age,tumor size,depth on invasion and histology were risk factors for the positive rate of peritoneal free cancer cells.Multivariate analysis showed depth on invasion,histology were independent factors for the positive rate of peritoneal free cancer cells.Conclusion Peritoneal free cells is the important factor of developing peritoneal metastasis,and the clinicopathological factors affect the positive rate of peritoneal free cancer cells of gastric cancer patients.

Objective The purpose of this study is to compare long-term survival of neoadjuvant chemotherapy followed by radical hysterectomy with concurrent chemoradiotherapy in locally advanced cervical cancer.Methods A total of 222 stageⅠB2-ⅡB locally advanced cervical cancer cases from January 2000 to December 2004 were analyzed retrospectivly.All the cases were divided into two groups:155 cases of neoadjuvant chemotherapy followed by radical hysterectomy with pelvic lymph node dissection(NACT + RS group);67 cases of concurrent chemoradiotherapy group(CCRT group).Patients were followed up from 54 to 114 months,NACT group patients were followed up for more than 72.6 months,all risk factors that may affect the disease-free survival(DFS)and overall survival(OS)were assessed.The NACT+RS group had significantly higher survival rates than both the CCRT(DFS:hazard ratio=2.765,P=0.0021;OS:hazard ratio=3.516,P=0.0002)groups.Results In this study,median follow-up time was 72.6 months,the five-year DFS rates of the NACT+RS and CCRT groups were 88.39% and 70.94%,respectively(P=0.006),while the five-year OS rates were 88.52% and 72.91%,respectively(P=0.0004).Conclusion There was no significant difference in both the short-term effect and complete remission rate between NACT + RS group and CCRT group on stage ⅠB2-ⅡB locally advanced cervical cancer patients;it was significantly better on DFS and OS of NACT + RS group than that of CCRT group.

Objective To investigate the inhibition and apoptotic effect of trihydroxybenzoic acid on HL-60 cells.Methods MTT-based cytotoxicity assay was used to test the HL-60 cells proliferation.The apoptotic cells were detected by AO fluorescent staining and flow cytometry;Results TBA inhibited HL-60 cells proliferation in a dose-dependent manner,HL-60 cells treated with TBA for 48h,AO fluorescent staining results showed that part of cell cytoplasm condensed,the volume was narrow,nuclear shrinkage,chromatin increased,more brightly fluorescent,condensed to form dense green fluorescent apoptotic features such as.Compared with control group(2.53±0.56)%,the percentage of early apoptotic cells was(28.24±5.24)%,the difference was significant(P＜0.05).Conclusion TBA inhibited the proliferation of HL-60 cells by inducing apoptosis.

Objective To investigate the expression and significance of Caspase-3 and FAK in hepatocarcinoma infected with Hepatitis B Virus after TACE.Methods To collect thirty-eight surgical specimens of hepatocarcinoma after TACE,including twenty-seven cases infected with HBV and eleven cases with no HBV.The Caspase-3 and FAK in residual tissues of hepatocarcinoma were detected by using immunohistochemical SP method.Results The positive rate of Caspase-3 and FAK in HBV group were respectively 22.2% and 81.5%,and that of no HBV group were 63.6% and 36.4% respectively.The difference between the two groups was statistically significant(P=0.024;P=0.017).The expression of Caspase-3 and FAK in HBV group was negatively correlated(r=-0.433,P=0.020).Conclusion HBV could suppress the expression of Caspase-3 and up-regulate the expression of FAK.HBV effect the therapeutic effect of TACE.

Objective To observe the clinical efficacy of endostar combined with cisplatin in the treatment of malignant hydrothorax and ascites.Methods Endostar combined with cisplatin(combined group)was applied in 42 cases of patients with malignant hydrothorax and ascites.40 cases of patients with malignant hydrothorax and ascites treated by cisplatin(control team).Following a maxnium release of hydrothorax and ascites,endostar and cisplatin or cisplatin were injected,to evaluate recent efficacy and toxicity.Results Among 42 cases(combined group),there were 18 cases achieved CR,20 cases of PR,objective efficiency rate was 90.4 %.Among 40 cases(control group),39 cases of patients were found to improve their quality of life(92.9%),control group was 75%,there was significant differences between two groups(P=0.018).36 cases of patients with the increased level of VEGF,decrease of ≥25% of the baseline had a significantly better response rate and QOL improvement rate than patients with a decrease of ＜25%(P=0.027,P=0.006).In two groups,the occurrence rates of G3/4 toxicities were mainly beacause of cisplatin,including nausea,vomiting and fatigue,there was no significant differences of them.Conclusion Endostar combined with cisplatin can better control the malignant hydrothorax and ascites,clinical safety is better,it is worthy of clinical application.