ERCC1，TUBB3在上皮性卵巢癌中表达及其临床病理特征和相关性的研究

doi:10.11904/j.issn.1002-3070.2014.04.002

实用肿瘤学杂志 ›› 2014, Vol. 28 ›› Issue (4): 294-298.doi: 10.11904/j.issn.1002-3070.2014.04.002

ERCC1，TUBB3在上皮性卵巢癌中表达及其临床病理特征和相关性的研究

陈可心李琛秦誉姚国栋耿敬姝

哈尔滨医科大学附属肿瘤医院病理科（哈尔滨150081）

出版日期:2014-08-28 发布日期:2014-07-31
作者简介:陈可心，男，（1987-），硕士研究生，从事肿瘤转移和耐药的分子机制研究

Study on the association between expression and clinical and pathological significance of ERCC1 and TUBB3 in epithelial ovarian carcinoma

CHEN Kexin, LI Chen, QIN Yu, YAO Guodong, GENG Jingshu

¹ Department of pathology, The Affiliated Tumor Hospital of Harbin Medical University，Harbin 150081，China

Online:2014-08-28 Published:2014-07-31

摘要/Abstract

摘要： 目的研究核苷酸切除修复交叉互补组1（ERCC1），TUBB3编码的β-tubulin-Ⅲ（3型β微管蛋白）在卵巢上皮性癌中的表达情况、病理特征及其临床意义，为个体化治疗提供一定的理论依据。方法本研究主要通过逆转录-实时荧光定量反应（RTq-PCR）方法检测上皮性卵巢癌组织中ERCC1，TUBB3 mRNA的表达情况，进一步分析上皮性卵巢癌临床病理特征与ERCC1，TUBB3的关系及二者之间的相关性。结果 ERCC1和TUBB3在上皮性卵巢癌均有高表达，表达率分别为28.21%（22/78）和29.49%（23/78）。单因素分析ERCC1、TUBB3基因在上皮性卵巢癌高表达患者中仅与残余肿物大小有关（P＜0.05），二者与其他临床病理因素无关。ERCC1和TUBB3在上皮性卵巢癌中表达水平呈中度正相关（r=0.4249，P<0.05）。结论上皮性卵巢癌组织存在不同水平的ERCC1和TUBB3基因mRNA，二者在癌组织中表达密切相关。ERCC1、TUBB3基因在上皮性卵巢癌中的表达与残余肿物大小呈正相关。

关键词: 上皮性卵巢癌, 核苷酸切除修复交叉互补组1, 3型β微管蛋白, 逆转录-实时荧光定量反应

Abstract: Objective To investigate the expression of ERCC1 and β-Tubulin-?Ⅲ in ovarian epithelial carcinoma and their correlation with pathological characteristics as well as clinical significance, thus to provide theoretical basis for ovarian epithelial carcinoma individualized treatment. Methods The mRNA expressions of ERCC1 and TUBB3 in ovarian epithelial cancer tissues were detected by reverse transcription real-time fluorescence quantitative reactin (RTq-PCR).We analyzed the correlation of the expression of ERCC1 mRNA and TUBB3 mRNA and clinical pathological features of patients. Results ERCC1 and TUBB3 positively expressed in epithelial ovarian cancer. The rate of high expression of ERCC1 was 28.21%（22/78）and that of TUBB3 high expression was 29.49%（23/78）. By univariate analysis, high expression of ERCC1 mRNA and TUBB3 mRNA was only correlated with the size of residual mass（P＜0.05）, but not other clinical and pathological features. ERCC1 and TUBB3 expression was moderately correlated with each other（r=0.4249，P<0.05）. Conclusion ERCC1 and TUBB3 positively expresses in epithelial ovarian cancer and they are correlated with each other. High expression of ERCC1 mRNA and TUBB3 mRNA is positively correlated with the size of residual mass.

Key words: Epithelial ovarian carcinoma, ERCC1, TUBB3, RTq-PCR

中图分类号:

R737.31

陈可心李琛秦誉姚国栋耿敬姝. ERCC1，TUBB3在上皮性卵巢癌中表达及其临床病理特征和相关性的研究[J]. 实用肿瘤学杂志, 2014, 28(4): 294-298.

CHEN Kexin,LI Chen,QIN Yu,YAO Guodong,GENG Jingshu. Study on the association between expression and clinical and pathological significance of ERCC1 and TUBB3 in epithelial ovarian carcinoma[J]. PRACTICAL ONCOLOGY JOURNAL, 2014, 28(4): 294-298.

[1]	吕博文, 钱钧, 王杰, 钱景荣, 卢莹莹, 苏丽菊, 杨桐树, 李文辉. miR-455-5p在上皮性卵巢癌中表达研究及其靶基因功能分析[J]. 实用肿瘤学杂志, 2019, 33(2): 115-121.
[2]	姜亮亮综述, 王晶审校. 上皮性卵巢癌远处转移的诊治进展[J]. 实用肿瘤学杂志, 2018, 32(5): 480-482.
[3]	李文辉, 吕博文, 钱钧, 苏丽菊, 杨桐树, 钱景荣, 王杰. MicroRNA-150对上皮性卵巢癌细胞增殖、凋亡和侵袭转移能力的影响[J]. 实用肿瘤学杂志, 2018, 32(3): 208-213.
[4]	毕琦玲, 周立红, 韩毅敏. HOX基因与妇科肿瘤的研究进展[J]. 实用肿瘤学杂志, 2018, 32(2): 140-143.
[5]	杨萌, 娄阁. AEG-1与Ki-67在上皮性卵巢组织中的表达及其临床病理相关性的研究[J]. 实用肿瘤学杂志, 2011, 25(5): 411-415.
[6]	朱素芳;苏秀兰. 上皮性卵巢癌患者外周血淋巴细胞亚群改变的研究[J]. 实用肿瘤学杂志, 2007, 21(05): 447-448.
[7]	秦薇;杨元先;王泽华. 增殖细胞核抗原与卵巢癌细胞系化学敏感性的关系[J]. 实用肿瘤学杂志, 2003, 17(02): 83-85.

ERCC1，TUBB3在上皮性卵巢癌中表达及其临床病理特征和相关性的研究

Study on the association between expression and clinical and pathological significance of ERCC1 and TUBB3 in epithelial ovarian carcinoma

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 7

编辑推荐

Metrics

本文评价