miR-455-5p在上皮性卵巢癌中表达研究及其靶基因功能分析

doi:10.11904/j.issn.1002-3070.2019.02.004

实用肿瘤学杂志 ›› 2019, Vol. 33 ›› Issue (2): 115-121.doi: 10.11904/j.issn.1002-3070.2019.02.004

miR-455-5p在上皮性卵巢癌中表达研究及其靶基因功能分析

吕博文¹, 钱钧², 王杰¹, 钱景荣¹, 卢莹莹¹, 苏丽菊³, 杨桐树¹, 李文辉¹

1.哈尔滨医科大学附属肿瘤医院(哈尔滨 150081);
2.哈尔滨医科大学;
3.哈尔滨市第一医院

收稿日期:2018-12-03 修回日期:2019-01-04 出版日期:2019-04-20 发布日期:2019-04-25
作者简介:吕博文,女,(1989-),硕士,住院医师,从事临床检验诊断的研究
基金资助:
黑龙江省留学归国人员科学基金(编号:LC2015035)

Expression of MiR-455-5p in epithelial ovarian cancer and its target gene function

LV Bowen¹, QIAN Jun², WANG Jie¹, QIAN Jingrong¹, LU Yingying¹, SU Liju³, YANG Tongshu¹, LI Wenhui¹

Harbin Medical University Cancer Hospital, Harbin 150081, China

Received:2018-12-03 Revised:2019-01-04 Online:2019-04-20 Published:2019-04-25

摘要/Abstract

摘要： 目的研究miR-455-5p在上皮性卵巢癌中的表达情况,分析其表达对上皮性卵巢癌发生发展的影响。方法从GEO数据库中下载正常卵巢上皮组织与上皮性卵巢癌组织miRNA表达数据GSE83693,通过差异表达分析获得上皮性卵巢癌中miRNA差异表达数据,分析miR-455-5p在正常卵巢上皮与上皮性卵巢癌组织中的表达是否存在差异,应用qRT-PCR验证差异表达预测结果;应用生物信息学软件对miR-455-5p靶基因进行KEGG通路富集分析及GO基因功能注释,探究miR-455-5p表达失调在上皮性卵巢癌发生发展过程中的作用。结果筛选出明显差异表达miRNA 101例,34例表达上调,67例表达下调;其中miR-455-5p呈明显下调(P＜0.01),差异倍数为-2.9019;qRT-PCR验证结果显示,上皮性卵巢癌细胞(SKOV-3,OVCAR-3,A2780)中miR-455-5p表达量明显低于正常卵巢上皮细胞(IOSE-80),差异均有统计学意义(P＜0.05);KEGG通路富集分析结果显示,miR-455-5p调控的靶基因主要参与的通路共5个,包括有TGF-β信号通路,Hippo信号通路,ECM-受体相互作用,癌症中的转录失调通路,慢性粒细胞白血病,这些通路均与肿瘤相关。GO功能注释分析结果显示上述通路中miR-455-5p调控的靶基因主要参与蛋白质磷酸化,促进细胞增殖、迁移,抑制细胞凋亡,促进上皮-间充质转化,转录调节及调控细胞周期等与肿瘤发生相关的功能。结论 miR-455-5p在上皮性卵巢癌中表达下调,miR-455-5p靶基因参与上皮性卵巢癌肿瘤相关通路与功能,与上皮性卵巢癌发生发展相关。

关键词: 上皮性卵巢癌, miR-455-5p, miRNA表达谱, KEGG通路富集分析, GO功能注释分析

Abstract: Objective The aim of this study was to investigate the expression of miR-455-5p in epithelial ovarian cancer and its effect on the development of epithelial ovarian cancer. Methods The miRNA expression data of normal ovarian epithelial tissues and epithelial ovarian cancer tissues GSE83693 were downloaded from the GEO database.Differential expression analysis was used to obtain differential expression data of miRNAs in epithelial ovarian cancer.The expression of miR-455-5p was analyzed whether there is difference expression between normal ovarian epithelium and epithelial ovary cancer tissues; qRT-PCR was used to verify the differential expression prediction results; bio-informatics software was used to analyze the KEGG pathway enrichment and GO gene function annotation of miR-455-5p target genes,and to explore the disorders of dyregulated miR-455-5p in the development of epithelial ovarian cancer. Results A total of 101 cases of differentially expressed miRNAs were screened,34 cases were up-regulated and 67 cases were down-regulated.Among them,miR-455-5p was down-regulated significantly(P＜0.01),and the different fulds were -2.9019.The results of qRT-PCR showed that the expression of miR-455-5p in epithelial ovarian cancer cells(SKOV-3,OVCAR-3 and A2780)was significantly lower than that in normal ovarian epithelial cells(IOSE-80),and the differential expression was statistically significant(P＜0.05).The results of KEGG pathway enrichment analysis showed that miR-455-5p regulated target genes mainly involved in five pathways,including TGF-β signaling pathway,Hippo signaling pathway,ECM-receptor interaction,transcriptional dysregulation pathway in cancer,and chronic granule cellular leukemia,which were associated with tumors.GO functional annotation analysis showed that the target genes regulated by miR-455-5p in the above pathway was mainly involved in protein phosphorylation,promoted cell proliferation and migration,inhibited apoptosis,promoted epithelial-mesenchymal transition,regulated transcription and regulated cell cycle,etc.,which associated with tumorigenesis. Conclusion The expression of miR-455-5p is down-regulated in epithelial ovarian cancer.The miR-455-5p target genes are involved in the pathogenesis and function of epithelial ovarian cancer,and are associated with the development of epithelial ovarian cancer.

Key words: Epithelial ovarian cancer, miRNA-455-5p, miRNA expression profile, KEGG pathway enrich analysis, GO functional annotation analysis

中图分类号:

R737.31

吕博文, 钱钧, 王杰, 钱景荣, 卢莹莹, 苏丽菊, 杨桐树, 李文辉. miR-455-5p在上皮性卵巢癌中表达研究及其靶基因功能分析[J]. 实用肿瘤学杂志, 2019, 33(2): 115-121.

LV Bowen, QIAN Jun, WANG Jie, QIAN Jingrong, LU Yingying, SU Liju, YANG Tongshu, LI Wenhui. Expression of MiR-455-5p in epithelial ovarian cancer and its target gene function[J]. PRACTICAL ONCOLOGY JOURNAL, 2019, 33(2): 115-121.

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miR-455-5p在上皮性卵巢癌中表达研究及其靶基因功能分析

Expression of MiR-455-5p in epithelial ovarian cancer and its target gene function

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