维吾尔族宫颈癌患者Th1/Th2型细胞因子表达水平及临床意义

doi:10.11904/j.issn.1002-3070.2016.05.002

实用肿瘤学杂志 ›› 2016, Vol. 30 ›› Issue (5): 391-395.doi: 10.11904/j.issn.1002-3070.2016.05.002

维吾尔族宫颈癌患者Th1/Th2型细胞因子表达水平及临床意义

盛磊¹, 武贵臻¹, 胡尔西旦·尼牙孜², 阿布力孜·阿布杜拉¹

1.新疆医科大学新疆地方病分子生物学重点实验室(乌鲁木齐 830011);
2.新疆医科大学第一附属医院肿瘤中心

收稿日期:2016-07-15 出版日期:2016-10-28 发布日期:2016-11-02
通讯作者: 阿布力孜·阿布杜拉,E-mail:abulizi_de@126.com
作者简介:盛磊,女,(1983-),博士研究生,从事宫颈癌发病机制与早期预警的研究
基金资助:
新疆维吾尔自治区自然科学基金(2014211C023)

The expression of Th1/Th2 type cytokines and clinical significance in Uyghur cervical cancer

SHENG Lei¹, WU Guizhen¹, HUERXIDAN Niyazi², ABULIZI Abudula¹

1.Xinjiang Key Laboratory of Molecular Biology and Endemic Diseases,Xinjiang Medical University,Urumqi 830011,China;
2.Cancer Center of The First Afiliated Hospital,Xinjiang Medical University

Received:2016-07-15 Online:2016-10-28 Published:2016-11-02

摘要/Abstract

摘要： 目的评估宫颈癌及癌前病变患者免疫水平,进一步探讨维吾尔族宫颈癌患者血浆中Th1/Th2型细胞因子表达水平及临床意义。方法本研究收集宫颈癌、癌前病变(CIN Ⅲ)患者及对照组外周血标本,使用ELISA法检测血浆中Th1型细胞因子IL-2、IFN-γ及Th2型细胞因子IL-4、IL-10的表达水平,绘制ROC曲线分析四种细胞因子在宫颈癌辅助诊断中的价值。结果与对照组相比,宫颈癌和癌前病变组Th1型细胞因子IL-2、IFN-γ表达量显著降低(P＜0.05),Th2型细胞因子IL-4、IL-10表达量显著升高(P＜0.05);在宫颈癌组中,IL-10表达量随肿瘤分期逐渐升高(P＜0.05);维吾尔族宫颈癌患者血浆IL-2表达量较汉族患者显著降低(P＜0.05);在区分宫颈癌组与对照组中,IL-2、IFN-γ、IL-4和IL-10的AUC分别为0.979、0.766、0.736和0.903。结论宫颈癌患者细胞免疫水平低下,体内发生Th1/Th2漂移,提示这可能是肿瘤细胞发生免疫逃逸的机制之一,Th1/Th2型细胞因子的检测对宫颈癌的辅助诊断具有一定参考价值,此外IL-2表达量的下调可能在维吾尔族宫颈癌发生发展过程中起到重要作用。

关键词: 宫颈癌, Th1/Th2, 免疫水平, 酶联免疫吸附实验

Abstract: Objective To assess the immune level of cervical cancer patients and precancerous lesions patients,and investigate the association of Th1 / Th2 type cytokines expression and clinical pathologic factors in the plasma of Uyghur cervical cancer patients.Methods we collected peripheral blood specimens from cervical cancer patients,precancerous lesion(CIN Ⅲ)patients and controls.The expressions of Th1 type cytokines IL-2,IFN-γand Th2 type cytokines IL-4,IL-10 in plasma were detected by ELISA.Receiver operating characteristic curve(ROC curve)was used to analyze the cytokines value of auxiliary diagnosis in cervical cancer.Results The Results showed that compared with control group,the expressions of Th1 type cytokines IL-2,IFN-γin cervical cancer and precancerous lesion group were significantly reduced(P＜0.05).The expressions of Th2 type cytokines IL-4,IL-10 in cervical cancer and precancerous lesion group were significantly increased(P＜0.05).in cervical cancer group,IL-10 expression gradually increased with tumor pathological staging(P＜0.05).IL-2 expression level in Uyghur cervical cancer patients was significantly lower than in Han patients(P＜0.05).Compared cervical cancer group with control group,AUC of IL-2,IFN-γ,IL-4 and IL-10 were 0.979,0.766,0.736 and 0.903.Conclusion Cellular immune level of cervical cancer patients was low and Th1/Th2 shift has occurred,which suggests that it may be one of the mechanisms in immune escape of tumor cells.Th1 / Th2 type cytokines detection has a certain significance for auxiliary diagnosis of cervical cancer.Besides,the decrease of IL-2 expression may play an important role in the occurrence and development of Uyghur patients with cervical cancer.

Key words: Cervical cancer, Th1/Th2, Immune level, ELISA

中图分类号:

R737.33

盛磊, 武贵臻, 胡尔西旦·尼牙孜, 阿布力孜·阿布杜拉. 维吾尔族宫颈癌患者Th1/Th2型细胞因子表达水平及临床意义[J]. 实用肿瘤学杂志, 2016, 30(5): 391-395.

SHENG Lei, WU Guizhen, HUERXIDAN Niyazi, ABULIZI Abudula. The expression of Th1/Th2 type cytokines and clinical significance in Uyghur cervical cancer[J]. PRACTICAL ONCOLOGY JOURNAL, 2016, 30(5): 391-395.

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维吾尔族宫颈癌患者Th1/Th2型细胞因子表达水平及临床意义

The expression of Th1/Th2 type cytokines and clinical significance in Uyghur cervical cancer

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