miR-219在舌鳞状细胞癌中的表达及意义研究

doi:10.11904/j.issn.1002-3070.2016.05.007

实用肿瘤学杂志 ›› 2016, Vol. 30 ›› Issue (5): 420-424.doi: 10.11904/j.issn.1002-3070.2016.05.007

miR-219在舌鳞状细胞癌中的表达及意义研究

苑振楠, 宋凯镔

哈尔滨医科大学附属肿瘤医院头颈外科(哈尔滨 150081)

收稿日期:2016-07-26 出版日期:2016-10-28 发布日期:2016-11-02
通讯作者: 宋凯镔,E-mail:11714398@qq.com
作者简介:苑振楠,男,(1986-),硕士,住院医师,从事头颈外科学的研究

Expression and significance of miR-219 in tongue squamous cell carcinoma

YUAN Zhennan, SONG Kaibin

Department of Head and Neck Surgery,The Affiliated Tumor Hospital of Harbin Medical University,Harbin 150081,China

Received:2016-07-26 Online:2016-10-28 Published:2016-11-02

摘要/Abstract

摘要： 目的探讨miR-219在舌鳞状细胞癌(Tongue squamous cell carcinoma,TSCC)中的表达及其在TSCC发生发展中的作用。方法利用qRT-PCR检测miR-219在26例TSCC组织及对应癌旁组织标本、舌磷癌细胞SCC-15及正常口腔黏膜细胞中的表达。在舌磷癌细胞SCC-15中转染miR-219基因,用MTT法、克隆形成实验、Transwell细胞迁移及侵袭实验检测miR-219过表达对舌磷癌细胞SCC-15增殖、克隆形成、迁移及侵袭的影响。结果 qRT-PCR结果显示,miR-219在TSCC组织中的表达水平明显低于对应正常癌旁组织(P＜0.001),舌磷癌细胞SCC-15中miR-219的表达水平亦低于正常口腔黏膜细胞(P＜0.05)。MTT结果表明,转染miR-219后,与对照组相比,舌磷癌细胞SCC-15的增殖受到明显抑制(P＜0.05)。克隆形成实验发现,miR-219过表达明显抑制了SCC-15细胞的克隆形成能力(P＜0.05)。同时miR-219上调也明显抑制了舌磷癌细胞SCC-15迁移及侵袭能力(P＜0.05)。结论在TSCC中miR-219表达降低;miR-219上调可抑制舌磷癌细胞SCC-15的增殖、克隆、迁移及侵袭能力。

关键词: 舌鳞状细胞癌, miR-219, 增殖, 迁移, 侵袭

Abstract: Objective To investigate the expression of miR-219 and its role in tongue squamous cell carcinoma(TSCC).Methods The relative expression of miR-219 in the 26 samples of surgically resected paired TSCC and normal tumor-adjacent tissues was detected by qRT-PCR.The expression of miR-219 in SCC-15 and normal oral mucosa cell were also tested by qRT-PCR.The miR-219 mimic was transferred into SCC-15 cell.Cell proliferative rate and the ability of colony formation were analyzed.The abilities of migration and invasion in SCC-15 cell were evaluated by Transwell test.Results Expression of miR-219 was significantly lower in TSCC tissues(P＜0.001)and SCC-15(P＜0.05)in comparison with normal tumor adjacent tissues and normal oral mucosa cell.The abilities of proliferation(P＜0.05),colony formation(P＜0.05),migrationand invasion(P＜0.05)were inhibited in miR-219-transferred SCC-15 cells.Conclusion The expression of miR-219 was downregulated in TSCC.The miR-219 could inhibit cell proliferation,colony formation,migration and invasion in TSCC.

Key words: Tongue squamous cell carcinoma, miR-219, Proliferation, Migration, Invasion

中图分类号:

R739.86

苑振楠, 宋凯镔. miR-219在舌鳞状细胞癌中的表达及意义研究[J]. 实用肿瘤学杂志, 2016, 30(5): 420-424.

YUAN Zhennan, SONG Kaibin. Expression and significance of miR-219 in tongue squamous cell carcinoma[J]. PRACTICAL ONCOLOGY JOURNAL, 2016, 30(5): 420-424.

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miR-219在舌鳞状细胞癌中的表达及意义研究

Expression and significance of miR-219 in tongue squamous cell carcinoma

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