miR-34a对结肠癌细胞SW480增殖、迁移和侵袭能力的影响及机制探讨

doi:10.11904/j.issn.1002-3070.2017.03.001

实用肿瘤学杂志 ›› 2017, Vol. 31 ›› Issue (3): 193-198.doi: 10.11904/j.issn.1002-3070.2017.03.001

• 基础研究 • 下一篇

miR-34a对结肠癌细胞SW480增殖、迁移和侵袭能力的影响及机制探讨

郝小军，王传卓，赵相轩，辛鹤，刘兆玉

中国医科大学附属盛京医院放射科(沈阳 110004)

收稿日期:2016-12-29 出版日期:2017-06-28 发布日期:2017-07-04
通讯作者: 刘兆玉,E-mail:liuzy@sj-hospital.org
作者简介:郝小军,男,(1990-),硕士研究生,从事肿瘤介入治疗和影像诊断的研究
基金资助:
国家自然科学基金(81470086)

Effects of miR-34a on proliferation,migration and invasion of colon cancer SW480 cell and its possible mechanism

HAO Xiaojun,WANG Chuanzhuo,ZHAO Xiangxuan,XIN he,LIU Zhaoyu

Department of Radiology,Shengjing Hospital of China Medical University,Shenyang 110004,China

Received:2016-12-29 Online:2017-06-28 Published:2017-07-04

摘要/Abstract

摘要： 目的探讨miR-34a对结肠癌细胞株SW480增殖、侵袭和迁移能力的影响及可能的作用机制。方法将miR-34a过表达慢病毒、空病毒载体转染SW480细胞,未做处理细胞作为空白对照组。Real-time PCR检测各组细胞内miR-34a的表达;CCK8法检测细胞增殖能力;划痕实验、Transwell小室实验检测细胞迁移和侵袭能力;Western blot实验检测细胞内E-cadherin和Vimentin蛋白表达。结果与空病毒载体组及空白对照组相比,转染组细胞中miR-34a的表达增高,且细胞增殖效率、侵袭和迁移能力降低(P＜0.05),miR-34a使E-cadherin蛋白表达增加、Vimentin蛋白表达降低。结论 miR-34a可抑制结肠癌细胞SW480增殖、侵袭和迁移能力,并能影响E-cadherin和Vimentin的表达,miR-34a有望成为干预结肠癌转移和复发的分子靶点。

关键词: 结直肠癌, miR-34a, EMT, 增殖, 侵袭

Abstract: Objective The objective of this study was to investigate effects of miR-34a on the proliferation,invasion and migration of colon cancer SW480 cell and its possible mechanism.Methods miR-34a overexpressed lentivirus and empty virus vector were transfected into SW480 cells and untreated cells were used as blank control group.Real-time PCR was used to detect the expression of miR-34a in each group.The cell proliferation was detected by CCK8 assay.The cell migration and invasion ability were detected by wound healing and transwell assays.The expression of E-cadherin and Vimentin protein was detected by Western blotting.Results Compared with the empty virus vector group and the blank control group,the expression of miR-34a was increased in the transfected cells,and the cell proliferation efficiency,invasion and migration ability were decreased in the transfected cells(P＜0.05).miR-34a significantly increased the expression of E-cadherin protein and decreased Vimentin protein expression in the transfected cells.Conclusion miR-34a can inhibit the proliferation,invasion and migration of colon cancer SW480 cells,and affect the expression of E-cadherin and Vimentin.MR-34a is expected to be a potential molecular target for the metastasis and recurrence of colorectal cancer.

Key words: Colorectal cancer, miR-34a, EMT, Proliferation, Invasion

中图分类号:

R735.3

郝小军，王传卓，赵相轩，辛鹤，刘兆玉. miR-34a对结肠癌细胞SW480增殖、迁移和侵袭能力的影响及机制探讨[J]. 实用肿瘤学杂志, 2017, 31(3): 193-198.

HAO Xiaojun,WANG Chuanzhuo,ZHAO Xiangxuan,XIN he,LIU Zhaoyu. Effects of miR-34a on proliferation,migration and invasion of colon cancer SW480 cell and its possible mechanism[J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(3): 193-198.

参考文献

1 Chen W,Zheng R,Baade PD,et al.Cancer statistics in China,2015[J].CA Cancer J Clin,2016,66(2):115-132.
2 Berindan Neagoe I,Monroig PC,Pasculli B,et al.MicroRNAome genome:a treasure for cancer diagnosis and therapy[J].Cancer J Clinicians,2014,64(5):311-336.
3 Kouso H,Yano T,Maruyama R,et al.Differences in the expression of epithelial-mesenchymal transition related molecules between primary tumors and pulmonary metastatic tumors in colorectal cancer[J].Surgery Today,2012,43(1):73-80.
4 Zhang D,Zhou J,Ming D.Dysregulation of microRNA-34a expression in colorectal cancer inhibits the phosphorylation of FAK via VEGF[J].Digest Dis Sci,2014,59(5):958-967.
5 Sahani DV,Bajwa MA,Andrabi Y,et al.Current status of imaging and emerging techniques to evaluate liver metastases from colorectal carcinoma[J].Annal Surgery,2014,259(5):861-872.
6 Lu J,Getz G,Miska EA,et al.MicroRNA expression profiles classify human cancers[J].Nature,2005,435(7043):834-838.
7 Cao W,Fan R,Wang L,et al.Expression and regulatory function of miRNA-34a in targeting survivin in gastric cancer cells[J].Tumor Biology,2013,34(2):963-971.
8 Si W,Li Y,Shao H,et al.MiR-34a inhibits breast cancer proliferation and progression by targeting Wnt1 in Wnt/β-catenin signaling pathway[J].Am J Med Sci,2016,352(2):191-199.
9 Li N,Fu H,Tie Y,et al.miR-34a inhibits migration and invasion by down-regulation of c-Met expression in human hepatocellular carcinoma cells[J].Cancer Letters,2009,275(1):44-53.
10 Gao J,Li N,Dong Y,et al.miR-34a-5p suppresses colorectal cancer metastasis and predicts recurrence in patients with stage Ⅱ/Ⅲ colorectal cancer[J].Oncogene,2015,34(31):4142-4152.
11 Nieto MA.Epithelial plasticity:a common theme in embryonic and cancer cells[J].Science,2013,342(6159):1234850.
12 Wrighton KH.Cell migration:EMT promotes contact inhibition of locomotion[J].Nat Rev Mol Cell Biol,2015,16(9):518.
13 Davalos V,Moutinho C,Villanueva A,et al.Dynamic epigenetic regulation of the microRNA-200 family mediates epithelial and mesenchymal transitions in human tumorigenesis[J].Oncogene,2012,31(16):2062-2074.

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miR-34a对结肠癌细胞SW480增殖、迁移和侵袭能力的影响及机制探讨

Effects of miR-34a on proliferation,migration and invasion of colon cancer SW480 cell and its possible mechanism

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