miR-409-5p对肝癌细胞系HepG2增殖及迁移的影响

doi:10.11904/j.issn.1002-3070.2017.04.004

实用肿瘤学杂志 ›› 2017, Vol. 31 ›› Issue (4): 305-309.doi: 10.11904/j.issn.1002-3070.2017.04.004

miR-409-5p对肝癌细胞系HepG2增殖及迁移的影响

王睿¹,余陈欢²,方杰²

1.杭州市中医院(杭州 310007);
2.浙江省医学科学院

收稿日期:2016-12-16 出版日期:2017-08-20 发布日期:2017-08-23
通讯作者: 余陈欢,E-mail:yuchenhuan2002@163.com
作者简介:王睿,男,(1987-),博士,主治医师,从事针灸推拿机制的研究。
基金资助:
1.浙江省院所专项(No.2015D3006);2.浙江省中医药科技计划项目(No.2015ZA161)

Effects of miR-409-5p on the proliferation and migration of hepatoma HepG2 cell line

WANG Rui¹,YU Chenhuan²,FANG Jie²

1.Hangzhou Hospital of Traditional Chinese Medicine,Hangzhou 310007,China;
2.Zhejiang Academy of Medicial Sciences

Received:2016-12-16 Online:2017-08-20 Published:2017-08-23

摘要/Abstract

摘要： 目的观察过表达miR-409-5p对肝癌细胞系HepG2增殖及迁移能力的影响。方法利用miR-409-5p mimics进行转染肝癌细胞系HepG2,并通过实时荧光定量PCR进行验证。高表达miR-409-5p后,使用MTT法和划痕实验分别检测HepG2细胞增殖能力及迁移能力的变化。结果 MTT实验显示,HepG2细胞转染miR-409-5p mimics后细胞增长趋势减缓,且随miR-409-5p mimics浓度和时间成正比。划痕实验显示,在24h和48h两个时间点三组间比较差异均有统计学意义(P＜0.05),证实过表达miR-409-5p mimics的细胞体外迁移能力明显低于转染阴性对照组(NC mimics)组和对照组。结论上调miR-409-5p可抑制肝癌细胞系HepG2的增殖及迁移能力。

关键词: miR-409-5p, 肝癌, 增殖, 迁移

Abstract: Objective The objective of this study was to observe effects of miR-409-5p on the proliferation and migration of HepG2 cells.Methods HepG2 cells were transfected with miR-409-5p mimics and verified by Real-Time quantitative PCR.After high expression of miR-409-5p,MTT and wound healing assays were used to detect the proliferation and migration ability of HepG2 cells.Results The cell viability of HepG2 cells transfected with miR-409-5p mimics was significantly decreased in comparison with the control group and showed a dose-and time-dependent manner(P＜0.05).The migration ability of cells overexpressing miR-409-5p mimics was significantly lower than that in the NC group(P＜0.05).There was significant difference between the two groups at treatments for 24 h and 48 h.Conclusion Up-regulated miR-409-5p can inhibit the proliferation and migration of HepG2 cells.

Key words: miR-409-5p, HepG2, Proliferation, Migration

中图分类号:

R735.7

王睿,余陈欢,方杰. miR-409-5p对肝癌细胞系HepG2增殖及迁移的影响[J]. 实用肿瘤学杂志, 2017, 31(4): 305-309.

WANG Rui ,YU Chenhuan ,FANG Jie. Effects of miR-409-5p on the proliferation and migration of hepatoma HepG2 cell line[J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(4): 305-309.

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miR-409-5p对肝癌细胞系HepG2增殖及迁移的影响

Effects of miR-409-5p on the proliferation and migration of hepatoma HepG2 cell line

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