Wnt通路抑制剂ETC-159处理对口腔鳞癌细胞增殖迁移的影响

doi:10.11904/j.issn.1002-3070.2018.02.002

实用肿瘤学杂志 ›› 2018, Vol. 32 ›› Issue (2): 103-106.doi: 10.11904/j.issn.1002-3070.2018.02.002

Wnt通路抑制剂ETC-159处理对口腔鳞癌细胞增殖迁移的影响

段艳军, 徐卫, 陈文杰, 王凤娟

襄阳市中心医院;湖北文理学院附属医院(襄阳 441000)

收稿日期:2017-11-28 出版日期:2018-04-28 发布日期:2018-05-09
通讯作者: 王凤娟,E-mail:851263718@qq.com
作者简介:段艳军,男,(1982-),本科,住院医师,从事口腔相关的研究。

Effects of Wnt pathway inhibitor ETC-159 on proliferation and migration of OSCC cells

DUAN Yanjun, XU Wei, CHEN Wenjie, WANG Fengjuan

Xiangyang Central Hospital,Affiliated Hospital of Hubei University of Arts and Science,Xiangyang 441000,China

Received:2017-11-28 Online:2018-04-28 Published:2018-05-09

摘要/Abstract

摘要：

目的研究Wnt通路抑制剂ETC-159对口腔鳞癌细胞系(SCC-15)增殖迁移的影响并探讨其机制。方法分别用DMSO和ETC-159处理SCC-15细胞12 h和24 h,用CCK8试剂盒检测细胞增殖活性,用Transwell小室检测细胞迁移能力,用Western blot法检测Wnt信号通路相关蛋白Wnt3a、β-catenin含量及Wnt通路调节的细胞增殖迁移相关蛋白c-Myc、cyclin D1、CD146含量。结果 ETC-159组处理SCC-15细胞12 h和24 h后,其增殖活性和迁移能力及Wnt3a、β-catenin、c-Myc、cyclin D1、CD146表达量均显著下降,与DMSO组相比差异具有统计学意义(P＜0.05)。结论 Wnt信号通路抑制剂ETC-159能通过降低c-Myc、cyclin D1、CD146的水平并抑制SCC-15细胞的增殖和迁移。

关键词: 口腔鳞癌, ETC-159, Wnt通路, 增殖, 迁移

Abstract:

Objective The Objective of this study was to investigate the effect of Wnt pathway inhibitor-ETC-159 on proliferation and migration of oral squamous cell carcinoma(SCC-15)cells and to explore its mechanism.Methods SCC-15 cells were treated with DMSO and ETC-159 for 12 h or 24 h.Cell proliferation was detected by CCK8 kit.Transwell assay was used to detect the ability of cell migration.Western blotting was used to detect cell migration related to proteins i.e.Wnt3a and β-catenin,proliferation and migration related proteins i.e.c-Myc,cyclin D1,CD146.Results After treated with ETC-159 for 12 or 24 h,the proliferation,migration and expression of Wnt3a,β-catenin,c-Myc,cyclin D1 and CD146 in SCC-15 cells were significantly decreased when compared to the DMSO group(P＜0.05).Conclusion Wnt signaling pathway inhibitor-ETC-159 can inhibit the proliferation and migration of SCC-15 cells by decreased levels of c-Myc,cyclin D1 and CD146.

Key words: Oral squamous cell carcinoma, ETC-159, Wnt pathway, Proliferation, Migration

中图分类号:

R739.85

段艳军, 徐卫, 陈文杰, 王凤娟. Wnt通路抑制剂ETC-159处理对口腔鳞癌细胞增殖迁移的影响[J]. 实用肿瘤学杂志, 2018, 32(2): 103-106.

DUAN Yanjun, XU Wei, CHEN Wenjie, WANG Fengjuan. Effects of Wnt pathway inhibitor ETC-159 on proliferation and migration of OSCC cells[J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(2): 103-106.

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Wnt通路抑制剂ETC-159处理对口腔鳞癌细胞增殖迁移的影响

Effects of Wnt pathway inhibitor ETC-159 on proliferation and migration of OSCC cells

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