沉默PRMT5基因对人肝癌细胞生物学行为的影响

doi:10.11904/j.issn.1002-3070.2020.03.004

实用肿瘤学杂志 ›› 2020, Vol. 34 ›› Issue (3): 208-213.doi: 10.11904/j.issn.1002-3070.2020.03.004

沉默PRMT5基因对人肝癌细胞生物学行为的影响

周健¹, 陈雪健¹, 王伟¹, 李宁¹, 孙振宇^{1, 2}, 徐力善¹

1.哈尔滨医科大学附属第四医院肿瘤外科肝胆外科(哈尔滨 150001);
2.浙江省宁波市第一医院肛肠外科

收稿日期:2020-01-02 修回日期:2020-03-26 发布日期:2020-06-30
作者简介:周健,男,(1992-),硕士研究生,从事消化系统肿瘤方面研究
基金资助:
黑龙江省科研计划项目(编号:201710);哈尔滨医科大学附属第四医院火炬计划(编号:HYDSYHJ201903)

The effect of silencing PRMT5 gene in biological behavior of human hepatocellular carcinoma cells

ZHOU Jian¹,CHEN Xuejian¹,WANG Wei¹,LI Ning¹,SUN Zhenyu^1.2,XU Lishan¹

1.Department of Oncological and Hepatobiliary Surgery,The Fourth Affiliated Hospital of Harbin Medical University,Harbin 150001,China;
2.Department of Anorectal Surgery,The First Hospital of Ningbo City

Received:2020-01-02 Revised:2020-03-26 Published:2020-06-30

摘要/Abstract

摘要： 目的探讨蛋白质精氨酸甲基转移酶5(Protein arginine methyltransferase 5,PRMT5)对人肝癌细胞生物学行为的影响。方法应用免疫印迹法(Western blot)检测正常肝细胞LO2、肝癌细胞HepG2及Huh7中PRMT5的表达水平,应用siRNA技术将PRMT5基因敲减,流式细胞仪检测肝癌细胞HepG2及Huh7的凋亡情况。应用Western blot检测蛋白激酶B(Protein kinase B,AKT)及磷酸化蛋白激酶B(Phosphorylated protein kinase B,P-AKT)的表达水平。结果与正常肝细胞LO2相比,PRMT5在肝癌细胞HepG2及Huh7中高表达(P＜0.05)。抑制PRMT5的表达可以促进肝癌细胞凋亡。将PRMT5基因敲减后,肝癌细胞系中P-AKT的表达量降低(P＜0.01)。结论 PRMT5可能通过调控肝细胞肝癌(Hepatocellular Carcinoma,HCC)中AKT通路从而促进肝癌细胞凋亡。因此,PRMT5可能是肝癌的潜在生物标志物和有希望的治疗靶标。

关键词: PRMT5, 肝癌细胞, P-AKT

Abstract: Objective To investigate the effect of protein arginine methyltransferase 5(PRMT5)on the biological behavior of human hepatocellular carcinoma cells.Methods The expression of PRMT5 was detected with Western blot in normal liver cell line LO2 and hepatocellular carcinoma cell lines HepG2 and Huh7 cells.siRNA technology was used to knockdown the expression of PRMT5 and flow cytometry was used to detect the apoptosis of HepG2 and Huh7 cells.The protein expression of protein kinase B(AKT)and phosphorylated protein kinase B(P-AKT)was detected by Western blot.Results Compared with LO2 cells,PRMT5 was highly expressed in hepatocellular carcinoma cell lines HepG2 and Huh7(P＜0.05).Inhibition of PRMT5 expression could promote apoptosis of HCC cells.The expression of P-AKT was decreased when knocked-down the expression of PRMT5 gene(P＜0.01).Conclusion PRMT5 may promote HCC(Hepatocellular Carcinoma)cell apoptosis by regulating AKT pathway.Therefore,PRMT5 may be a potential biomarker and promising therapeutic target for hepatocellular carcinoma.

Key words: PRMT5, Hepatocellular carcinoma cells, P-AKT

中图分类号:

R735.7

周健, 陈雪健, 王伟, 李宁, 孙振宇, 徐力善. 沉默PRMT5基因对人肝癌细胞生物学行为的影响[J]. 实用肿瘤学杂志, 2020, 34(3): 208-213.

ZHOU Jian,CHEN Xuejian,WANG Wei,LI Ning,SUN Zhenyu,XU Lishan. The effect of silencing PRMT5 gene in biological behavior of human hepatocellular carcinoma cells[J]. Journal of Practical Oncology, 2020, 34(3): 208-213.

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链接本文: http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/10.11904/j.issn.1002-3070.2020.03.004

http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/Y2020/V34/I3/208

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沉默PRMT5基因对人肝癌细胞生物学行为的影响

The effect of silencing PRMT5 gene in biological behavior of human hepatocellular carcinoma cells

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