FOXD3在肺癌细胞中的作用

doi:10.11904/j.issn.1002-3070.2019.05.002

摘要/Abstract

摘要： 目的研究FOXD3对肺癌细胞系A549增殖和迁移的影响。方法在TCGA数据库中对FOXD3在多种肿瘤中的表达情况进行分析。使用在线数据库GEPIA评估FOXD3 mRNA表达对患者预后影响。构建FOXD3过表达肺癌细胞系并进行功能学实验检测FOXD3在肺癌中的作用。使用STRING数据库对FOXD3参与的调控网络进行预测。结果通过比对肿瘤的表达谱显示,FOXD3在肿瘤中具有差异性表达,其中FOXD3在皮肤黑色素瘤(SKCM)中表达上调,FOXD3在肿瘤中表达下调的有:结肠腺癌(COAD)、直肠腺癌(READ)、睾丸生殖细胞瘤(TGCT)。使用GEPIA数据库对肿瘤患者生存进行研究发现FOXD3与肺鳞癌(LUSC)、皮肤黑色素瘤(SKCM)、肾上腺皮质瘤(ACC)以及低分化胶质瘤(LGG)肿瘤患者总生存期有关。FOXD3能够抑制肺癌细胞增殖和迁移侵袭能力;通过使用STRING数据库发现FOXD3能够参与多种基因的表达调控。结论 FOXD3过表达抑制肺癌细胞增殖和迁移侵袭能力并与患者预后密切相关,因此FOXD3能够作为指示肺癌良好预后的标志,并且为肿瘤治疗提供新思路。

关键词: FOXD3, 肺癌, 预后

Abstract: Objective The aim of this study was to explore effects of FOXD3 on proliferation and migration of lung cancer A549 cells.Methods The expression of FOXD3 in various tumors was analyzed in the TCGA database.The online database GEPIA was used to assess the prognostic impact of FOXD3 mRNA expression.FOXD3 overexpressing lung cancer cell line was constructed and functional assay was performed to detect the role of FOXD3 in lung cancer cells.The STRING database was used to predict the regulatory network in which FOXD3 is involved.Results By comparing the expression profiles of tumors,among them,FOXD3 is up-regulated in tumors with skin cutaneous melanoma(SKCM),and FOXD3 is down-regulated in tumors:colon adenocarcinoma(COAD),rectal adnocarcinoma(READ)and testicular germ cell tumor(TGCT).Through the using of the GEPIA database to study the survival of cancer patients,FOXD3 was found to have an impact on the overall survival of patients with lung squamous cell carcinoma(LUSC),SKCM,adrenocortical carcinoma(ACC),and lower grade glioma(LGC).FOXD3 could inhibit the proliferation and migration of lung cancer cells.FOXD3 could be involved in the regulation of expression of various genes by using the STRING database.Conclusion FOXD3 overexpression inhibits proliferation and migration of lung cancer cells is closely associated with the prognosis of patient.Therefore,FOXD3 can be a good indicator for indicating good prognosis of lung cancer and provide new ideas for cancer treatment.

Key words: FOXD3, Lung cancer, Prognosis

中图分类号:

R734.1

刘四卫, 崔玥, 孙元元, 胡晶, 张文睿, 曹孟儒. FOXD3在肺癌细胞中的作用[J]. 实用肿瘤学杂志, 2019, 33(5): 390-396.

LIU Siwei, CUI Yue, SUN Yuanyuan, HU Jing, ZHANG Wenrui, CAO Mengru. Effect of FOXD3 in lung cancer cells[J]. Journal of Practical Oncology, 2019, 33(5): 390-396.

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链接本文: http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/10.11904/j.issn.1002-3070.2019.05.002

http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/Y2019/V33/I5/390

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