食管鳞癌中microRNA let-7a-3甲基化与IGF-Ⅱ的相关性

doi:10.11904/j.issn.1002-3070.2024.03.005

实用肿瘤学杂志 ›› 2024, Vol. 38 ›› Issue (3): 173-178.doi: 10.11904/j.issn.1002-3070.2024.03.005

食管鳞癌中microRNA let-7a-3甲基化与IGF-Ⅱ的相关性

朱公建¹, 郭红云¹, 白悦¹, 王晓敏², 朱小康¹, 刘东彦¹, 刘玉琴¹

1.甘肃省医学科学研究院/中山大学附属肿瘤医院甘肃医院科教科(兰州 730050);
2.兰州市西固区人民医院消化内科

收稿日期:2023-11-07 修回日期:2024-05-31 出版日期:2024-06-28 发布日期:2024-07-30
通讯作者: 刘玉琴,E-mail:liuyq970930@126.com
作者简介:朱公建,男,(1980-),博士,研究员,从事肿瘤分子流行病学的研究。
基金资助:
兰州市人才创业创新项目(编号:2018-RC-96)

The correlation between microRNA let-7a-3 methylation and IGF-Ⅱ expression in esophageal squamous cell carcinoma

ZHU Gongjian¹, GUO Hongyun¹, BAI Yue¹, WANG Xiaomin², ZHU Xiaokang¹, LIU Dongyan¹, LIU Yuqin¹

1. Department of Science and Education,Gansu Provincial Academy of Medical Science/ Sun Yat-sen University Cancer Center Gansu Hospital,Lanzhou 730050,China;
2. Department of Gastroenterology,Xigu District of Lanzhou City People′s Hospital

Received:2023-11-07 Revised:2024-05-31 Online:2024-06-28 Published:2024-07-30

摘要/Abstract

摘要： 目的探讨食管鳞癌组织中microRNA let-7a-3甲基化状态与血浆中类胰岛素样生长因子2(Insulin like growth factor 2,IGF-Ⅱ)表达的相关性。方法采用甲基化特异性PCR法(Methylation specific PCR,qMSP)检测83例食管癌及相对应的癌旁正常组织中let-7a-3甲基化状态,采用酶联免疫吸附试验(Enzyme linked immunosorbent assay,ELISA)检测血浆中IGF-Ⅱ的表达水平。结果 83例食管鳞癌患者癌组织中的microRNA let-7a-3甲基化程度显著高于癌旁正常组织(P＜0.001)。83例食管鳞癌患者血浆中IGF-Ⅱ的表达水平与let-7a-3基因的甲基化程度总体上呈正相关,具有统计学意义(r=0.600,P＜0.001)。结论 microRNA let-7a-3可能通过对下游分子的甲基化调控参与食管鳞癌的发生发展,这对了解食管鳞癌形成的机制具有重要意义,可为食管鳞癌的诊断和预后提供依据。

关键词: 食管鳞癌, let-7a-3, 甲基化, 类胰岛素样生长因子2

Abstract: Objective The aim of this study was to explore the correlation between the methylation status of microRNA let-7a-3 in esophageal squamous cell carcinoma (ESCC)and the expression of insulin-like growth factor 2 (IGF-Ⅱ). Methods The methylation specific PCR (qMSP)was used to detect the methylation status of let-7a-3 in 83 cases of esophageal cancer and corresponding adjacent normal tissues.The enzyme linked immunosorbent assay (ELISA)was used to detect the expression of IGF-II in plasma. Results The degree of let-7a-3 methylation in cancer tissues of 83 patients with ESCC was significantly higher than that in normal tissues adjacent to cancer (P＜0.001).The expression of IGF-Ⅱ in the plasma of 83 patients with ESCC was positively correlated with the methylation degree of let-7a-3,which was statistically significant (r=0.600,P＜0.001). Conclusion microRNA let-7a-3 may participate in the occurrence and progression of ESCC by regulating the methylation of downstream molecules,which is of great significance for understanding the mechanisms of ESCC development and providing a basis for the diagnosis and prognosis of ESCC.

Key words: Esophageal squamous cell carcinoma, let-7a-3, Methylation, Insulin like growth factor 2

中图分类号:

R735.1

朱公建, 郭红云, 白悦, 王晓敏, 朱小康, 刘东彦, 刘玉琴. 食管鳞癌中microRNA let-7a-3甲基化与IGF-Ⅱ的相关性[J]. 实用肿瘤学杂志, 2024, 38(3): 173-178.

ZHU Gongjian, GUO Hongyun, BAI Yue, WANG Xiaomin, ZHU Xiaokang, LIU Dongyan, LIU Yuqin. The correlation between microRNA let-7a-3 methylation and IGF-Ⅱ expression in esophageal squamous cell carcinoma[J]. Journal of Practical Oncology, 2024, 38(3): 173-178.

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食管鳞癌中microRNA let-7a-3甲基化与IGF-Ⅱ的相关性

The correlation between microRNA let-7a-3 methylation and IGF-Ⅱ expression in esophageal squamous cell carcinoma

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