TRa1基因转录后小片段P28、P43在甲状腺乳头状癌中的表达及临床意义

doi:10.11904/j.issn.1002-3070.2017.04.002

实用肿瘤学杂志 ›› 2017, Vol. 31 ›› Issue (4): 294-298.doi: 10.11904/j.issn.1002-3070.2017.04.002

TRa1基因转录后小片段P28、P43在甲状腺乳头状癌中的表达及临床意义

李仕亮¹,徐雷²,邵国安²,孙纷纷³,孙林庸⁴,薛军⁵

1.攀枝花攀钢集团总医院甲状腺乳腺外科(攀枝花 617000);
2.新疆医科大学第五附属医院甲状腺乳腺外科;
3.攀枝花攀钢集团总医院肾内科;
4.华西医院病理科;5.临汾市人民医院病理科

收稿日期:2016-12-10 出版日期:2017-08-20 发布日期:2017-08-23
通讯作者: 李仕亮,E-mail:1002679824@qq.com
作者简介:李仕亮,男,(1987-),硕士,住院医师,从事肿瘤基础与临床的研究。
基金资助:
新疆维吾尔自治区自然科学基金项目(2012221A050)

Expression and clinical significance of small fragments of P28 and P43 in papillary thyroid carcinoma after TRa1 gene transcription

LI Shiliang¹,XU Lei²,SHAO Guoan²,SUN Fenfen³,SUN Linyong⁴,XUE Jun⁵

1.Department of Thyroid and Breast Surgery,The Panzhihua General Hospital of Panzhihua Iron and Steel Group,Panzhihua 617000,China;
2.Department of Thyroid and Breast Surgery,The Fifth Affiliated Hospital,Xinjiang Medical University;
3.Department of Kidney Internal Medicine,The Panzhihua General Hospital of Panzhihua Iron and Steel Group;
4.Department of Pathology,The West China Hospital;5.Department of Pathology,The Linfen people′s Hospital

Received:2016-12-10 Online:2017-08-20 Published:2017-08-23

摘要/Abstract

摘要： 目的分析TRa1基因转录后小片段P28、P43在甲状腺乳头状癌(Papillary thyroid carcinoma,PTC)组织中的表达水平及其与临床病理的关系。方法采用实时荧光定量PCR法(RT-PCR)和凝胶电泳法分别扩增目的片段和分离不同片段的条带,再利用紫外透射分析仪扫描各条带的光密度来检测31例PTC组织及癌旁组织中Mt-P28、Mt-P43的表达情况。结果甲状腺癌组Mt-P28平均灰度值(0.77±0.34)明显高于癌旁组(0.31±0.18);Mt-P43平均灰度值(0.85±0.21)明显高于癌旁组(0.34±0.15),差异均具有统计学意义(P＜0.05);Mt-P28表达水平与患者性别、年龄、肿瘤大小、淋巴结转移及临床分期无关(P＞0.05);Mt-P43表达水平与患者性别、年龄、肿瘤大小、淋巴结转移无关(P＞0.05)而与临床分期有关(P＜0.05)。Mt-P28与Mt-P43两者表达水平不具有相关性(r=0.266,P=0.071)。结论 Mt-P28、Mt-P43表达水平的升高倾向于病变恶性程度更高,对预测PTC不良预后有一定的临床应用价值,两种因子对于PTC的预防和治疗有指导意义。

关键词: 甲状腺乳头状癌, Mt-P28, Mt-P43, RT-PCR

Abstract: Objective The objective of this study was to investigate the expression of P28 and P43 in papillary thyroid carcinoma(PTC)and its relationship with clinicopathological features after TRa1 gene transcription.Methods Real-time fluorescence quantitative PCR(RT-PCR)and gel electrophoresis were used to determine the target fragments and to isolate the bands of different fragments.The optical density of each band was scanned by UV transmittance analyzer to detect 31 cases of PTC tissue and expression levels of P28 and P43 in para-cancerous tissues.Results The average gray value of P28 in thyroid carcinoma group was 0.77±0.34,which was significantly higher than that in para-cancer group(0.31±0.18).The average gray value of P43(0.85+0.21)in thyroid carcinoma group was significantly higher than that in para-cancer group(0.34±0.15)(P＜0.05).The expression levels of P28 were not correlated with gender,age,tumor size,lymph node metastasis and clinical pathologic stage(P＞0.05),The expression levels of P43 were not correlated with gender,age,tumor size and lymph node metastasis.(P＞0.05)but they were related to clinical pathologic stage(P＜0.05).There was no correlation between expression levels of P28 and P43(r=0.266,P=0.071).Conclusion The increased expression of P28 and P43 may have a high degree of malignancy and a certain clinical value in predicting the adverse prognosis of PTC.Both factors are helpful for the prevention and treatment of PTC.

Key words: Papillary thyroid carcinoma, P28, P43, RT-PCR

中图分类号:

R736.1

李仕亮,徐雷,邵国安,孙纷纷,孙林庸,薛军. TRa1基因转录后小片段P28、P43在甲状腺乳头状癌中的表达及临床意义[J]. 实用肿瘤学杂志, 2017, 31(4): 294-298.

LI Shiliang ,XU Lei ,SHAO Guoan ,SUN Fenfen ,SUN Linyong ,XUE Jun. Expression and clinical significance of small fragments of P28 and P43 in papillary thyroid carcinoma after TRa1 gene transcription[J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(4): 294-298.

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TRa1基因转录后小片段P28、P43在甲状腺乳头状癌中的表达及临床意义

Expression and clinical significance of small fragments of P28 and P43 in papillary thyroid carcinoma after TRa1 gene transcription

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