牙髓干细胞外泌体对口腔鳞状细胞癌CAL-27增殖、迁移的影响及其机制的研究

doi:10.11904/j.issn.1002-3070.2022.05.002

实用肿瘤学杂志 ›› 2022, Vol. 36 ›› Issue (5): 398-403.doi: 10.11904/j.issn.1002-3070.2022.05.002

牙髓干细胞外泌体对口腔鳞状细胞癌CAL-27增殖、迁移的影响及其机制的研究

岳杰, 杨麒麟, 孙翔宇, 孙誉铭, 麻响, 陈东, 静广平

哈尔滨医科大学附属第一医院口腔颌面外科(哈尔滨 150001)

收稿日期:2022-03-24 修回日期:2022-09-28 出版日期:2022-10-28 发布日期:2022-11-10
通讯作者: 静广平,E-mail:jinggp2003@163.com
作者简介:岳杰,男,(1995-),硕士研究生,从事口腔鳞状细胞癌治疗的研究。
基金资助:
哈尔滨医科大学附属第一医院科研创新基金(编号:2019M22)

Effects of exosomes derived from dental pulp stem cells on the proliferation and migration of oral squamous cell carcinoma CAL-27 and its mechanism

YUE Jie, YANG Qilin, SUN Xiangyu, SUN Yuming, MA Xiang, CHEN Dong, JING Guangping

The First Affiliated Hospital of Harbin Medical University,School of Stomatology,Harbin Medical University,Harbin 150001,China

Received:2022-03-24 Revised:2022-09-28 Online:2022-10-28 Published:2022-11-10

摘要/Abstract

摘要： 目的研究人牙髓干细胞来源的外泌体(human dental pulp stem cell-derived exosome,hDPSCs-exo)对口腔鳞状细胞癌细胞(CAL-27)增殖和迁移的影响。方法收集来哈尔滨医科大学附属第一医院治疗的16~28岁患者因阻生或正畸拔除的牙髓组织,通过超速离心法提取hDPSCs-exo,不同浓度hDPSCs-exo处理CAL-27细胞,通过MTT实验、Transwell实验和划痕实验检测CAL-27细胞增殖和迁移能力的变化;Western blot研究PI3K/Akt信号通路相关蛋白的表达。结果 MTT实验结果显示,仅80 μg/mL hDPSCs-exo组在第5d对CAL-27细胞的增殖有抑制作用(P＜0.05),其余各浓度组均没有显著影响;Transwell和划痕实验结果显示20、60、80 μg/mL组可以显著地促进CAL-27细胞的迁移(P＜0.05);Western blot结果显示经hDPSCs-exo处理后,CAL-27细胞中PI3K/Akt信号通路相关蛋白PI3K、P-Akt的表达水平明显上调(P＜0.05)。结论 hDPSCs-exo高浓度时抑制CAL-27细胞的增殖,适宜浓度能显著促进CAL-27细胞的迁移能力,其作用机制可能与激活PI3K/Akt信号通路有关。

关键词: 牙髓干细胞, 外泌体, 口腔鳞状细胞癌, PI3K/Akt, 增殖, 迁移

Abstract: Objective The objective of this study was to investigate the effects of human dental pulp stem cell derived exosomes (hDPSCs-exo) on the proliferation and migration of oral squamous carcinoma CAL-27 cells. Methods hDPSCs-exo was extracted by ultracentrifugation.CAL-27 cells were treated with different concentrations of hDPSCs-exo.The proliferation and migration of CAL-27 cells were detected by MTT assay,Transwell and scratch assays;Western Blot was used to detect the levels of PI3K/AKT signaling pathway related proteins. Results The results of MTT assay showed that only 80 μg/mL of hDPSCs-exo had an inhibitory effect on the proliferation of CAL-27 cells on the 5th day (P＜0.05),and the other concentration groups had no significant effect.The results of Transwell and scratch assays showed that 20,60,and 80 μg/mL of hDPSCs-exo could significantly promote migration of CAL-27 cells (P＜0.05).The results of Western blot showed that the levels of PI3K/AKT signaling pathway related proteins PI3K and P-AKT were significantly up-regulated in CAL-27 cells after hDPSCs-exo treatment(P＜0.05). Conclusion High concentration of hDPSCs-exo can inhibit the proliferation of CAL-27 cells,and the appropriate concentration can significantly promote the migration of CAL-27 cells.Its mechanism of action may be related to the activation of the PI3K/AKT signaling pathway.

Key words: Dental pulp stem cells, Exosomes, Oral squamous cell carcinoma, PI3K/Akt, Proliferation, Migration

中图分类号:

R739.8

岳杰, 杨麒麟, 孙翔宇, 孙誉铭, 麻响, 陈东, 静广平. 牙髓干细胞外泌体对口腔鳞状细胞癌CAL-27增殖、迁移的影响及其机制的研究[J]. 实用肿瘤学杂志, 2022, 36(5): 398-403.

YUE Jie, YANG Qilin, SUN Xiangyu, SUN Yuming, MA Xiang, CHEN Dong, JING Guangping. Effects of exosomes derived from dental pulp stem cells on the proliferation and migration of oral squamous cell carcinoma CAL-27 and its mechanism[J]. Journal of Practical Oncology, 2022, 36(5): 398-403.

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http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/Y2022/V36/I5/398

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牙髓干细胞外泌体对口腔鳞状细胞癌CAL-27增殖、迁移的影响及其机制的研究

Effects of exosomes derived from dental pulp stem cells on the proliferation and migration of oral squamous cell carcinoma CAL-27 and its mechanism

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