上调miR-222对肝癌细胞系HepG2增殖和侵袭能力的影响

doi:10.11904/j.issn.1002-3070.2015.03.010

Abstract

Abstract:

Objective To detect the changes of cell invasion and proliferation in hepatocellular carcinoma cell line HepG2 through the upregulation of miR-222.Methods The expression of miR-222 was up-regulated by liposomal transfection.The expression of PTEN which is the targeting protein of miR-222 was detected by using Western Blot assay.The changes of proliferative capacity and invasive ability in HepG2 cells after the upregulation of miR-222 were detected through MTT assay and Transwell assay.Results In Western Blot experiments,the expression of PTEN in transfection groups were lower than that in negative control groups and blank control groups.In MTT assay,the OD values in transfected group were higher than that in negative control group and blank control group(P＜0.05).In transwell experiments,the number of penetrating cells in transfected group were higher than that in negative control group and blank control group(P＜0.05).Conclusion Our Results suggest that PTEN is significantly down regulated by the upregulation of miR-222.The proliferative capacity and invasive ability are raised in hepatocellular carcinoma cell line HepG2 through the upregulation of miR-222.

Key words: MicroRNA, miR-222, PTEN, Hepatocellular carcinoma

CLC Number:

TAO Xuanchen,MA Donglai,WU Dequan. Effect of up-regulating miR-222 in HepG2 cells on its invasion and proliferation[J]. PRACTICAL ONCOLOGY JOURNAL, 2015, 29(3): 235-238.

References

1 陈万青,邹小农,张思维.中国肝癌死亡率地理分布分析[J].实用肿瘤学杂志,2008,22(3):201-203.
2 Jemal A,Bray F,Center MM,et al.Global cancer statistics[J].CA Cancer J Clin,2011,61(2):69-90.
3 曹孟儒,蔡莉.miRNA与肿瘤[J].实用肿瘤学杂志,2009,23(6):597-599.
4 贾树钊,杨树萌,杨艳梅,等.PTEN在三氧化二砷诱导肝癌细胞(SMMC-7721)凋亡中的作用[J].实用肿瘤学杂志,2012,26(4):302-304,310.
5 Garofalo M,Di Leva G,Romano G,et al.miR-221&222 regulate TRAIL resistance and enhance tumorigenicity through PTEN and TIMP3 downregulation[J].Cancer Cell,2009,16(6):498-509.
6 Zhou Q,Lui VW,Yeo W.Targeting the PI3K/Akt/mTOR pathway in hepatocellular carcinoma[J].Future Oncol,2011,7(10):1149-1167.
7 Wong QW,Ching AK,Chan AW,et al.MiR-222 overexpression confers cell migratory advantages in hepatocellular carcinoma through enhancing AKT signaling[J].Clin Cancer Res,2010,16(3):867-875.
8 Karakatsanis A,Papaconstantinou I,Gazouli M,et al.Expression of microRNAs,miR-21,miR-31,miR-122,miR-145,miR-146a,miR-200c,miR-221,miR-222,and miR-223 in patients with hepatocellular carcinoma or intrahepatic cholangiocarcinoma and its prognostic significance[J].Mol Carcinog,2011,52(4):297-303.
9 Chiang HR,Schoenfeld LW,Ruby JG,et al.Mammalian microRNAs:experimental evaluation of novel and previously annotated genes[J].Genes Dev,2010,24(10):992-1009.
10 Inui M,Martello G,Piccolo S.MicroRNA control of signal transduction[J].Nat Rev Mol Cell Biol,2010,11(4):252-263.
11 李琼,王阁,王红中,等.MicroRNA在HepG2肝癌细胞表达差异谱的研究[J].重庆医学,2007,36(20):2024-2025.
12 Ma D,Tao X,Gao F,et al.miR-224 functions as an onco-miRNA in hepatocellular carcinoma cells by activating AKT signaling[J].Oncol Lett,2012,4(3):483-488.
13 孙建民.肝癌早期筛查肿瘤标记物研究进展[J].实用肿瘤学杂志,2013,27(1):93-96.

[1]	HE Wei, MU Qiming, WANG Gang, WAN Bo, LIAO Bo, YANG Juan. Expression of TACC3 and p53 protein in primary liver cancer and its clinical significance [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(5): 420-425.
[2]	SHAO Jiayue,SUN Xiuwei. Effect of allicin combined with 5-FU on apoptosis of colon cancer HT-29 cells and its mechanism [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(4): 289-292.
[3]	CHEN Zhaohong,HE Yingying,ZHANG Youcai,LU Dingyu. Effects of SOX7 overexpression on proliferation and other biological characteristics of gallbladder carcinoma GBC-SD cells and its mechanism [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(4): 293-297.
[4]	FAN Ningning, GENG Jingshu. The expression and role of LncRNA MEG3 in human glioma cells [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(3): 219-223.
[5]	SHI Xiaokang, DING, Youming. Research progress on the relationship between helicobacter and primary hepatocellular carcinoma [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(3): 271-274.
[6]	WEN Juanjuan, QU Bing, LIU Chao. Clinical significance of antithrombin Ⅲ gene expression in hepatocellular carcinoma tissues [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(2): 117-121.
[7]	XIN He, WANG Chuanzhuo, LIU Zhaoyu. Research progress on the relationship between microRNA-196a and tumor [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(1): 42-46.
[8]	WANG Jiana, SHEN Jianjun, ZHANG Yanhong, WANG Jiatian, LI Wenxiu, PAN Junying. Expression of Ki-67 and PTEN in oral adenoid cystic carcinoma and its clinical significance [J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(6): 489-493.
[9]	ZHAO Lingyun, QI Lunan, YOU Xuemei. Research progress of heat shock protein 70 in hepatocellular carcinoma [J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(6): 528-532.
[10]	ZHOU Chunfang,ZHAO Shanshan,SUN Zequn,WU Jun,ZHA Huolong,YUAN Yahong. Acid conditions induces EMT of hepatocellular carcinoma HepG2 cells [J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(3): 199-204.
[11]	ZHAO Menglei. Effects of lycopene on the proliferation and apoptosis in human hepatocellular carcinoma HepG2 cells [J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(3): 211-216.
[12]	HUANG Jian,PANG Hui,SONG Yuhang,CAI Li. The role of microRNA in drug resistance of breast cancer [J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(3): 272-276.
[13]	JIN Jing,HE Yutong. Research progress of miR-21 in esophageal cancer [J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(3): 277-280.
[14]	YANG Xiaowen, YU Aiqing, YANG Yi. Nanog influences the proliferative ability of HepG2 cells through the modulation of the cell cycle related proteins [J]. PRACTICAL ONCOLOGY JOURNAL, 2016, 30(5): 425-429.
[15]	LI Shijie, YANG Yi, TIAN Yulong, HOU Jiye, LIU Ruibao. The expression and significance of β-catenin and p28GANK in residual hepatocellular carcinoma cells after transcatheter arterial chemoembolization [J]. PRACTICAL ONCOLOGY JOURNAL, 2016, 30(5): 430-435.

Effect of up-regulating miR-222 in HepG2 cells on its invasion and proliferation

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments