Biglycan及FAK信号通路对结肠癌细胞侵袭、转移能力的影响及机制

doi:10.11904/j.issn.1002-3070.2015.05.013

Abstract

Abstract: Objective To observe the influence of Biglycan on the focal adhesion kinase(FAK)activation and to explore whether it regulates the invasion and metastasis of colon cancer through FAK signaling pathway.Methods The overexpressive plasmid of Biglycan was constructed and then transfected into the colon cancer cell line HCT116.Meanwhile,FAK inhibitor was used to treat cells.Control group(HCT116),empty plasmid group(Vector),empty plasmid and inhibitor treatment group(Vector+PF-562271),Biglycan overexpression group(Biglycan),Biglycan overexpression and inhibitor treatment group(Biglycan+PF-562271)were set.Twenty four hours after exposure to inhibitor,the expression of FAK and p-FAK in each group was detected by Western Blot.The invasion and metastasis of colon cancer cells was detected by transwell assay.Results Overexpression of Biglycan significantly enhanced the phosphorylation level of FAK and promoted the invasion and metastasis of HCT116(P＜0.01).The inhibitor of FAK PF-562271 could significantly reduce the expression of p-FAK and reverse the effect of Biglycan on the invasion and metastasis of colon cancer cells.Conclusion Biglycan regulates the invasion and metastasis of colon cancer cells through activation FAK signaling pathway.

Key words: Biglycan, FAK, Colon cancer, Invasion, Metastasis

CLC Number:

R735.3

GU Xiaohu, XIAO Jingdong, XING Xiaojing. Effects and mechanism of Biglycan and FAK signaling pathway on the invasion and metastasis of colon cancer cells[J]. PRACTICAL ONCOLOGY JOURNAL, 2015, 29(5): 444-449.

References

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Effects and mechanism of Biglycan and FAK signaling pathway on the invasion and metastasis of colon cancer cells

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