番茄红素对荷卵巢癌大鼠免疫功能的影响及其机制研究

doi:10.11904/j.issn.1002-3070.2017.01.003

Abstract

Abstract: Objective To investigate the effects of Lycopene(LP)on immune function of ovarian cancer rats' and its mechanism.Methods Sixty ovarian cancer rat models made by injecting ovarian cancer cell line NUTU-19 were divided into five groups randomly:model group,Cisplatin group(2 mg/kg)and LP(40,20,10 mg/kg)group,n=12;the LP and Cisplatin were given by intraperitoneal injection,once every two days for 5 times.The rats general states and tumor growth conditions were observed,the tumor tissue morphological changes was observed by H&E staining;the tumor weight,inhibition rate,spleen lymphocyte transformation rate were detected;and the content of IL-2 and TNF-α in serum were detected.The present of CD4⁺,CD8⁺ in blood were detected and the ratio of CD4⁺/CD8⁺ was calculated.Results The food situation of the rats in LP groups was improved,tumor was reduced,activity and hardness were poorer,necrosis presented,tumor cell shrinkage and other pathological morphological changes appeared,especially the rats in LP 40 mg/kg group.Compared with model group,the tumor weight in LP(40,20 mg/kg)groups was decreased,the inhibition rate and the spleen lymphocyte transformation rate were increased;the contents of IL-2 and TNF-α were decreased;the CD8⁺ present was decreased,while the CD4⁺ present in LP 40 mg/kg group was increased,the ratio of CD4⁺/CD8⁺ was increased.All of the difference above were significant(P＜0.05,P＜0.01).Conclusion LP can effectively improve the immune function of ovarian cancer rat;which perhaps relates to its effects of reducing the inflammatory cytokines levels and increasing the ratio of CD4⁺/CD8⁺.

Key words: Lycopene, Ovarian cancer, Rat, Immune function, Mechanism

CLC Number:

R737.3

CHAI Jingbo, LI Ping, PEI Zhiping. The effects and mechanism of Lycopene on immune function of ovarian cancer rats[J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(1): 13-17.

References

1 Jemal A,Siegel R,Xu J,et al.Cancer Statistics,2010[J].Ca Cancer Journal for Clinicians,2010,60(5):277-300.
2 Karachaliou N,Pilotto S,Bria E,et al.Platelets and their role in cancer evolution and immune system[J].Transl Lung Cancer Res,2015,4(6):713-720.
3 Codony-Servat J,Rosell R.Cancer stem cells and immunoresistance:clinical implications and solutions[J].Transl Lung Cancer Res,2015,4(6):689-703.
4 De Stefani E,Boffetta P,Oreggia F.Plant foods and risk of laryngeal cancer:a case-control study in Uraguay[J].Int J cancer,2000,87:129-132.
5 Lin HY,Huang BR,Yeh WL,et al.Antineuroinflammatory effects of lycopene via activation of adenosine monophosphate-activated protein kinase-α1/heme oxygenase-1 pathways[J].Neurobiol Aging,2014,35(1):191-202.
6 Aydin S,Tokac M,Taner G,et al.Antioxidant and antigenotoxic effects of lycopene in obstructive jaundice[J].J Surg Res,2013,182(2):285-295.
7 邹党华,谭布珍,黄艳琴,等.小剂量雷公藤内酯醇对荷卵巢癌大鼠体内抑瘤作用的观察[J].江西医学院学报,2009,49(2):110-111.
8 官佳懿,张永红,崔德凤,等.绿原酸对小鼠脾脏淋巴细胞体外增殖和分泌功能的影响[J].中国农学通报,2013,29(29):27-31.
9 陈慰峰.医学免疫学[M].第3版.北京:人民卫生出版社,2001:46-52.
10 Boyman O,Sprent J.The role of interleukin2 during homeostasis and activation of the immune system[J].Nat Rev Immunol,2012,12(3):180-190.
11 Wang Z,Wang G,Zhu X,et al.Interleukin-2 is upregulated in patients with a prolapsed lumbar intervertebral disc and modulates cell proliferation,apoptosis and extracellular matrix metabolism of human nucleus pulposus cells[J].Exp Ther Med,2015,10(6):2437-2443.
12 张建武,闵冬雨,周云,等.番茄红素对H₂O₂致乳鼠心肌细胞氧化应激损伤的保护作用[J].中国实验方剂学杂志,2014,20(12):160-164.
13 Anquetil F,Clavel C,Offer G,et al.IgM and IgA rheumatoid factors purified from rheumatoid arthritis sera boost the Fc receptor-and complement-dependent effector functions of the disease-specific anti-citrullinated protein autoantibodies[J].J Immunol,2015,194(8):3664-3674.
14 Xie J,Qiu ZF,Han Y,et al.The correlation between HLA-DR or CD38 expression in CD8(+)T cells and CD4(+)T cells count in treatment-naive HIV/AIDS patients[J].Zhonghua Nei Ke Za Zhi,2016,55(1):36-39.
15 Liu YL,Wu YB,Zhai K,et al.Determination of interleukin 27-producing CD4 and CD8 T cells for the differentiation between tuberculous and malignant pleural effusions[J].Sci Rep,2016,6:19424-19428.

[1]	QIN Wen,ZHAO Nannan,HAN Yonghuan,XU Yajun. Expression of ZNF436 in gastric cancer and its effect on the migration and invasion of gastric cancer cells by WNT/β-catenin [J]. PRACTICAL ONCOLOGY JOURNAL, 2019, 33(3): 222-227.
[2]	LIU Zhiqiang,CHU Yanjie,LIU Jing,WANG Yanbo. Atractylenolide I regulates proliferation and invasion of lung cancer A549 cells through the TLR4/MyD88 pathway [J]. PRACTICAL ONCOLOGY JOURNAL, 2019, 33(3): 228-232.
[3]	ZHANG Maoxiang,SUN Huixin,SONG Bingbing. Analysis of the incidence and mortality of malignant tumors in Daoli District,Harbin,2008—2012 [J]. PRACTICAL ONCOLOGY JOURNAL, 2019, 33(3): 261-265.
[4]	LI Shan,ZHAO Chunbo,QIAO Wenbo. Progress in the application of intraoperative radiotherapy in pancreatic cancer [J]. PRACTICAL ONCOLOGY JOURNAL, 2019, 33(3): 280-283.
[5]	LV Bowen, QIAN Jun, WANG Jie, QIAN Jingrong, LU Yingying, SU Liju, YANG Tongshu, LI Wenhui. Expression of MiR-455-5p in epithelial ovarian cancer and its target gene function [J]. PRACTICAL ONCOLOGY JOURNAL, 2019, 33(2): 115-121.
[6]	BAI Xiaobin, HUO Longwei, XIE Wanfu, XU Gaofeng, WANG Maode. Expression of Chk1 in glioblastoma and its association with tumor biological behavior and prognosis survival [J]. PRACTICAL ONCOLOGY JOURNAL, 2019, 33(2): 122-127.
[7]	YUAN Guixia, LI Yan, FENG Wanqi, XIA Xiaojuan, LI Xu. Mechanism of tumor suppressor gene DKK2 inhibiting proliferation of nephroblastoma cells in children [J]. PRACTICAL ONCOLOGY JOURNAL, 2019, 33(1): 14-20.
[8]	ZHANG Fengchun, JIANG Ying, LIU Zhaonan, YAN Ningning, CUI Hongquan, XU Yingchun. Prognostic significance of neutrophil/lymphocyte ratio and platelet/lymphocyte ratio in systemic inflammation-related indicators in gastric cancer patients after radical resection [J]. PRACTICAL ONCOLOGY JOURNAL, 2019, 33(1): 34-39.
[9]	CHANG Xiaoxia, SONG Bingbing, LIU Jiaren. Research progress in phytochemicals for increasing sensitivity of tumor cells to chemotherapies [J]. PRACTICAL ONCOLOGY JOURNAL, 2019, 33(1): 67-72.
[10]	SONG Ling, FU Qiong. Study of the effect of astragalin on proliferation of ovarian cancer cells by inhibiting the glycolytic pathway induced Via HIF-1α [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(6): 503-509.
[11]	KAN Jun, ZHOU Daihan, ZHANG Enxin. Research progress in tumor with cardiovascular disease [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(6): 558-561.
[12]	LIU Juan, ZHANG Shutian. Advances in research on the mechanism of cyclin-dependent kinase inhibitor 3(CDKN3)in tumors [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(6): 575-578.
[13]	LI Wanzhen, YIN Jun, LI Ping, LIN Xiaohui, ZHANG Guoqian, ZHANG Shuxu. The expression and role of miR-17 in radioresistant cells of non-small cell lung cancer [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(5): 391-397.
[14]	CHEN Lin, WANG Enxiang, LIU Qunyou, ZHONG Ying, ZHOU Guoliang, ZHAO Xiyan. CDX2 gene inhibits of colon cancer cell proliferation by NF-κB signaling pathway [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(5): 398-403.
[15]	WANG Yuan, SUN Yihua. Advance in the role of β-catenin in soft tissue tumors [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(5): 455-459.

The effects and mechanism of Lycopene on immune function of ovarian cancer rats

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments