流式细胞仪检测恶性淋巴瘤患者外周血T细胞亚群的临床研究

doi:10.11904/j.issn.1002-3070.2017.05.008

Abstract

Abstract:

Objective The aim of this study was to investigate the effect of flow cytometry on peripheral blood T cell subsets in patients with malignant lymphoma and its relationship with clinicopathological and tumor types.Methods Ninety-eight patients with malignant lymphoma treated in our hospital from August 2014 to September 2016 were selected as the study group.Ninety-eight healthy subjects were selected as the control group.The peripheral blood T cell subsets(CD3⁺,CD4⁺,CD8⁺,CD4⁺/CD8⁺)were detected in patients and healthy controls by flow cytometry.Results The levels of CD3⁺ and CD4⁺/CD8⁺ in the study group were(55.63±11.25)and(1.32±0.62),respectively,which were significantly lower than those(68.96±12.63)and(1.59±0.59)of the control group(P＜0.05).The levels of CD4⁺ and CD8⁺ were(33.67±8.14)and(26.02±4.67),respectively in the study group,were no difference from the control group(34.12±8.33)and(25.67±4.53)(P＞0.05).The levels of CD3⁺ and CD4⁺/CD8⁺ in patients with Hodgkin's lymphoma were(54.63±11.36),(1.22±0.65),respectively,and(55.52±12.02),(1.34±0.71)for non-hodgkin lymphoma.They were significantly decreoseg in the control group(68.96±12.63 for CD3⁺ and 1.59±0.59 for CD4⁺/CD8⁺)(P＜0.05).The level of CD4⁺ and CD8⁺were no difference amoupst Hodgkin's lymphoma(33.78±8.23 for CD4⁺ and 25.74±4.88 for CD8⁺),non-Hodgkin's lymphoma(25.74±4.88 for CD4⁺ and 33.62±8.74 for CD8⁺)and control group(34.12±8.33 for CD4⁺ and 25.67±4.53 for CD8⁺)(P＞0.05).The levels of CD3⁺,CD4⁺ and CD4⁺/CD8⁺ in patients with Ⅲ ~Ⅳ stage malignant lymphoma were(52.66±12.47),(28.25±6.32)and(1.30±0.62),respectively,which were significantly lower than those(68.96±12.63),(34.12±8.33)and(1.59±0.59)in the control group(P＜0.05).The level of CD3⁺ in patients with phase I-II malignant lymphoma(58.63±11.85)was significantly lower than that in the control group(68.96±12.63)(P＜0.05).The level of CD8⁺ in patients with phase I-II malignant lymphoma(29.63±3.57)was significantly higher than that in the control group(25.67±4.53)(P＜0.05).Conclusion The detection of peripheral blood T cell subsets by flow cytometry can be used as an important methods to diagnose the disease,staging and immune status of patients with malignant lymphoma,which has high application value.

Key words: Flow cytometry, Malignant lymphoma, Peripheral blood T cell subsets

CLC Number:

R733

WANG Jingsheng, LIU Zhengjun, CHENG Nan, LI Dan, GAO Huiguang. Clinical study of peripheral blood T cell subsets in patients with malignant lymphoma by flow cytometry[J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(5): 431-435.

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Clinical study of peripheral blood T cell subsets in patients with malignant lymphoma by flow cytometry

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