Abstract: Objective To explore the clinical application value of flow-cytometry combined with colposcope ultra high frequency electric knife for diagnosis of uterine cervix carcinoma(UCC)and cervical intraepithelial neoplasia(CIN).Methods A total of 705 cases was included in this study who were screened from April 2014 to April 2016 and accepted colposcopy biopsy.DNA flow cytometry was performed to determine DNA index(DI)and proliferative index(PI).Results Colposcopy diagnosis Results showed that the detection rate of CINⅠ,CINⅡ,CINⅢ/CIS and UCC were 62.2%,78.0%,62.2% and 78.0% respectively.The sensitivity and specificity were 87.7%(213/243)and 90.2%(417/462)for DNA ploidy test using flow-cytometry.DI and PI of CINⅠ,CINⅡ,CINⅢ/CIS and UCC increased gradually with significant differences(P＜0.05).Conclusion Flow-cytometry combined with colposcopy biopsy may be a useful tool for cervical cancer screening in developing countries and has a competitive sensitivity and specificity,which is worthy of popularization and application.

Key words: Flow cytometry, Colposcopy biopsy, Uterine cervix carcinoma, Screening