长链非编码RNA Linc00152在泛癌中表达、预后及功能研究

doi:10.11904/j.issn.1002-3070.2018.01.002

Abstract

Abstract:

Objective The aim of this study was to explore the expression,prognosis and related function of Linc00152 in breast cancer. Methods Thirty-three cases of breast specimens were selected for RNA-Sequencing.The expression of Linc00152 was detected by qPCR in 50 pairs of breast cancer tissues and adjacent tissues.Combined with GEO and TCGA databases,the correlation between Linc00152 expression and the degree of malignancy and the prognosis of patients was analyzed.Cell proliferation,apoptosis and cell migration were detected in breast cancer MDA-MB-231 cell line,gastric cancer SGC-7901 cell line and renal cell carcinoma 786-O cell line. Results Linc00152 was highly expressed in breast cancer(P＜0.001),and was higher in HER2 positive and triple negative breast cancer(P＜0.001).In patients with high expression of Linc00152,the event-free survival and the metastasis-free survival were very poor(P＜0.001,P＜0.01).After knockdown Linc00152,the cell proliferation,migration and invasion were decreased and the apoptosis was increased(P＜0.05). Conclusion Linc00152 has a role of promoting cancer in malignant tumors and may be a potential therapeutic target.

Key words: Linc00152, Breast cancer, Expression, Prognosis, Phenotypic experiments

CLC Number:

R737.9

ZHENG Wei, XU Shouping, PANG Da. Expression,prognosis and function of long chain non-coding RNA-Linc00152 in breast cancer[J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(1): 7-13.

References

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Expression,prognosis and function of long chain non-coding RNA-Linc00152 in breast cancer

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