CDX2基因通过NF-κB信号通路抑制结肠癌细胞增殖

doi:10.11904/j.issn.1002-3070.2018.05.003

Abstract

Abstract: Objective The aim of this study was to observe the effects of overexpressing CDX2 gene on proliferation of human colon cancer HT-29 cell line and to explore its mechanism.Methods CDX2 was transfected into HT-29 cells by gene transfection technology,and stable transfected cell clones with stably expressing CDX2 gene were obtained by G418 selection.The expression of CDX2 was detected by RT-PCR and Western blotting.The biological behavior of transfected cells were analyzed by methythiazoletertraolium(MTT)assay and flow cytometry.Western blot assay was used to detect the expression of cyclin D1 and NF-κB phosphorylation level in HT-29 cells with overexpressing CDX2 gene.Results A system for stably overexpressing CDX2 human colon cancer HT-29 cell line was established.Compared with the nontransfected and empty vector transfected HT-29 cells,the growth rate of the cells in the transfected CDX2 group was significantly slower(P＜0.05).The proportion of G₁/G₀ phase of cell cycle was significantly increased(P＜0.05),and the proportion of S phase was significantly decreased(P＜0.05).Compared with the non-transfected and empty vector transfected cells,the expression of cyclin D1 and p-NF-κB activity were significant low in overexpressing CDX2 cells.Conclusion CDX2 gene may inhibit the proliferation of HT-29 cells through blocking the activity of NF-κB pathway and down-regulation of cyclin D1 protein expression.

Key words: Colorectal carcinoma, Proliferation, CDX2, NF-κB

CLC Number:

R735.3

CHEN Lin, WANG Enxiang, LIU Qunyou, ZHONG Ying, ZHOU Guoliang, ZHAO Xiyan. CDX2 gene inhibits of colon cancer cell proliferation by NF-κB signaling pathway[J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(5): 398-403.

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CDX2 gene inhibits of colon cancer cell proliferation by NF-κB signaling pathway

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