Abstract: Objective The Objective of this study was to investigate the effect of microRNA-409-3p(miRNA-409-3p)expression on proliferation of cervical cancer Hela cells and chemotherapy sensitivity of cisplatin.Methods HeLa cells were divided into normal control,miRNA-409-3p mimic and RNA control groups.The mRNA expression of miRNA-409-3p was detected by RT-qPCR.The cell viability was detected by CCK-8 assay,and the expression of Fip200,LC3 and Caspase-3 was detected by Western blot.Results The relative expression level of microRNA-409-3p mRNA in cervical cancer tissues was significantly lower than that in normal cervical tissues and adjacent tissues(P＜0.05).After transfection of microRNA-409-3p mimic,the relative expression level of microRNA-409-3p mRNA in the microNA-409-3p mimic group was significantly up-regulated compared with the normal control group(P＜0.05).The relative expression level of miRNA-409-3p mRNA in the RNA control group was not different from that in the normal control group(P＞0.05).The proliferative rate of HeLa cells int the microRNA-409 mimic group was significantly lower than that in normal control and RNA control groups(P＜0.05).After cisplatin treatment,the proliferation of Hela cells was significantly inhibited in the miRNA-409-3p mimic group(P＜0.05);the expression of Fip200 protein and LC3II/LC3I ratio in the microRNA-409-3p mimic group were significantly lower than those in the normal control and RNA control groups(P＜0.05).Conclusion The low expression of microRNA-409-3p in cervical cancer tissue may be related to the occurrence and development of cervical cancer.Upregulation of microRNA-409-3p level can inhibit the proliferation of HeLa cells and increase the sensitivity of HeLa cells to cisplatin.

Key words: miRNA-409-3p, Cervical cancer, Cell proliferation, Cisplatin sensitivity