ABL2在肺癌中的作用及机制研究

doi:10.11904/j.issn.1002-3070.2019.06.004

Abstract

Abstract: Objective The aim of this study was to investigate the role and mechanism of ABL2 in lung cancer and its mechanism.Methods The expression of ABL2 in lung cancer and adjacent tissues was detected by Real-Time PCR.A lung adenocarcinoma A549 cell line stably expressing of ABL2 was established,and the changes of cell proliferation and migration ability were detected by MTT,cell migration and colony formation assays.Western blot was used to detect the expression of EMT,apoptosis and PI3K/AKT signaling pathway-related proteins.Results The expression of ABL2 in lung cancer tissues was significantly higher than that in adjacent tissues(P＜0.001).After silencing ABL2 in the A549 cells,compared with the control group,the migration ability of cells was weakened after 48 hours(P＜0.001),the growth rate of cells began to slow down from the third day(P＜0.05),and the average number of clones formed after 15 days also decreased(P＜0.01).The expression of E-cadherin(P＜0.001)was increased in the epithelial cell marker after silencing ABL2,and the expression of stromal cell markers N-cadherin(P＜0.001),Vimentin(P＜0.01)and Snail(P＜0.001)was decreased.The expression of apoptosis-related protein Bcl-XL(P＜0.01)was decreased and BAX(P＜0.001)expression was up-regulated.The expression of PI3K/AKT signaling pathway-associated proteins such as PI3K P110(P＜0.05),AKT(P＜0.01)and p-AKT(P＜0.05)was significantly decreased.Conclusion Silencing ABL2 gene can promote apoptosis,and inhibit proliferation and migration of lung cancer cells through a PI3K/AKT signaling pathway.

Key words: Lung cancer, ABL2, Apoptosis

CLC Number:

R734.2

LI Chuanhai, LIU Yu, WANG Yanqun, CHEN Yingjian. The role and mechanism of ABL2 in lung cancer[J]. Journal of Practical Oncology, 2019, 33(6): 497-501.

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URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2019.06.004

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2019/V33/I6/497

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The role and mechanism of ABL2 in lung cancer

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