促进乳腺癌增殖转移和肿瘤干细胞特征的研究

doi:10.11904/j.issn.1002-3070.2020.06.007

Abstract

Abstract: Objective The Objective of this study were to analyze the expression of Gankyrin in breast tumors and its molecular mechanism to promote tumor progression. Methods The database was used to detect the expression of Gankyrin in breast cancer,and the correlation between its expression and patient survival was analyzed.After breast cancer BT549 and MDA-MB-231 cells overexpressed and knocked down the Gankyrin gene,CCK-8,Transwell and flow cytometry were used to analyze the cell proliferation,metastasis and the proportion of cancer stem cells. Results Analysis of the database showed that Gankyrin was highly expressed in breast cancer tissues,and its high expression was related to the poor prognosis of patients.Compared with normal breast tissues,the level of Gankyrin promoter methylation in breast cancer tissues was low.By overexpressing and knocking down the Gankyrin gene in breast cancer cells,Gankyrin had the ability to affect breast tumor cell proliferation and metastasis,and could maintain the tumor stem cell characteristics of breast cancer cells. Conclusion The high expression of Gankyrin in breast cancer is related to the poor prognosis of patients,and it may be used as a tumor marker of breast cancer.

Key words: Gankyrin, Breast cancer, Proliferation, Metastasis, Prognosis

CLC Number:

R737.9

NIU Ming, YU Ge. Gankyrin promotes tumor proliferation,metastasis and cancer stem cell characteristic in breast cancer[J]. Journal of Practical Oncology, 2020, 34(6): 518-523.

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Gankyrin promotes tumor proliferation,metastasis and cancer stem cell characteristic in breast cancer

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