基于生物信息学方法分析LAYN在甲状腺癌中临床预后价值及对免疫细胞浸润的影响

doi:10.11904/j.issn.1002-3070.2021.04.012

Abstract

Abstract: Objective The objective of this study was to explore the correlation between the expression of LAYN gene in thyroid cancer tissues and clinical prognosis,and its influence on infiltration of immune cell,so as to provide a basis for further research on potential molecular mechanism. Methods The genes related to thyroid cancer were collected from Oncomine and GEPIA databases,and analyzed the differences of the expression of LAYN gene between thyroid cancer tissues and normal tissues.The GEPIA database was used to analyze the correlation between the LAYN gene and the clinical stage,and prognosis of patients with thyroid cancer.The combination of ImmucellAI and TIMER2.0 tools was used to analyze the relationship between the LAYN gene and CD4⁺T cells and CD8⁺T cells infiltration abundance in thyroid cancer tissues.Combination with the STRING database was used to analyze the LAYN expression related proteins.Real-time fluorescent quantitative PCR(RT-qPCR)was used to further verify and detect the expression of LAYN in thyroid cancer tissues and adjacent tissues,thyroid cancer cell lines SW579,TPC-1 and thyroid epithelial TEC cells. Results The results of Oncomine,GEPIA and STRING database showed that compared with normal thyroid tissues,LAYN gene was low expressed in thyroid cancer tissues(P＜0.05).The LAYN gene was related to the clinical TNM staging of thyroid cancer(P＜0.05),and the LAYN gene was low expressed in tumor issues of patients with stage Ⅲ/Ⅳ.The overall survival(OS)of patients in the high expression of LAYN gene group was poor,while the disease free survival(DFS)was better(P＜0.05).In thyroid cancer tissues,the infiltration abundance of CD4⁺T cells and CD8⁺T cells was low,and the infiltration abundance of CD4⁺T cells and CD8⁺T cells was positive correlation with the expression of LAYN gene(P＜0.05).In addition,the proteins related to the expression of LAYN were PNRC1,MAGEH1,HAPLN1,SMOC1,PRSS12,HMMR,NF2,TLN2,TLN1 and TLE2.The verification results of RT-PCR showed that LAYN was low expressed in thyroid cancer and thyroid cancer SW579 and TPC-1 cell lines(P＜0.05). Conclusion The LAYN gene is low expressed in thyroid cancer tissues,and it is related to the clinical TNM staging and prognosis of patients with thyroid cancer.It can be used as a molecular marker for prognostic judgment.The underlying mechanism of this gene may play a role in affecting the abundance of infiltrating immune cells.This study provides a theoretical basis for studying the pathogenesis of thyroid cancer and the development of targeted drugs.

Key words: Thyroid cancer, LAYN gene, Clinical prognosis

CLC Number:

R736.1

YU Youlin, YU Junlin, HU Chaohua, SHEN Xiongshan, LI Weimin, LU Ting, YANG Feng, YANG Qingqing, LIU Chenhao. Analysis for clinical prognostic value of LAYN in thyroid cancer and its influence on infiltration of immune cells[J]. Journal of Practical Oncology, 2021, 35(4): 357-364.

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Analysis for clinical prognostic value of LAYN in thyroid cancer and its influence on infiltration of immune cells

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