Hsa-let-7b-5p抑制非小细胞肺癌的增殖、迁移和侵袭

doi:10.11904/j.issn.1002-3070.2024.03.003

Abstract

Abstract: Objective The objective of this study was to explore the values of hsa-let-7b-5p in the clinical diagnosis and targeted therapy of non-small cell lung cancer(NSCLC). Methods Thirteen pairs of NSCLC tissues and their adjacent tissues with the same pathological type in Harbin Medical University Cancer Hospital from September to October 2020 were collected,and differentially expressed miRNAs were obtained by RNA-Seq,and the expression of candidate miRNAs were verified in NSCLC tissues and cells through qRT-PCR.The effects of miRNAs on the proliferation,migration and invasion ability of NSCLC were verified by CCK-8 assay,clone formation assay,scratch assay and Transwell assay.Target genes were predicted using MiRDB,TarBase and ENCORI databases,and gene enrichment analysis was performed using FunRich tool. Results Compared with adjacent tissues and human bronchial epithelial cells(HBE),hsa-let-7b-5p was downregulated in NSCLC tissues and cell lines(A549 cells,H1299 cells,H358 cells and H460 cells)(P＜0.05).The high expression of hsa-let-7b-5p inhibited the proliferation,migration and invasion ability of NSCLC(P＜0.01).The results of target genes and enrichment analysis showed that among 378 target genes and related genes of hsa-let-7b-5p,147 genes were negatively correlated with the expression of hsa-let-7b-5p(P＜0.05). Conclusion Hsa-let-7b-5p has inhibitory effects on the proliferation,migration and invasion of NSCLC.

Key words: Hsa-let-7b-5p, Non-small cell lung cancer, Proliferation, Migration, Invasion, Biomarker

CLC Number:

R734.2

CHENG Yongming, DONG Huanhuan, XU Hui. Hsa-let-7b-5p inhibits proliferation,migration and invasion in non-small cell lung cancer[J]. Journal of Practical Oncology, 2024, 38(3): 157-167.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2024.03.003

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2024/V38/I3/157

References

1 Sung H,Ferlay J,Siegel RL,et al.Global cancer statistics 2020:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J].CA Cancer J Clin,2021,71(3):209-249.
2 Catalanotto C,Cogoni C,Zardo G.MicroRNA in control of gene expression:an overview of nuclear functions[J].Int J Mol Sci,2016,17(10):1712.
3 Boyerinas B,Park SM,Hau A,et al.The role of let-7 in cell differentiation and cancer[J].Endocr Relat Cancer,2010,17(1):19-36.
4 Duan S,Li J,Tian J,et al.Crosstalk between let-7a-5p and BCL-xL in the initiation of toxic autophagy in lung cancer[J].Mol Ther Oncolytics,2019,15:69-78.
5 Gunel T,Dogan B,Gumusoglu E,et al.Regulation of HMGA2 and KRAS genes in epithelial ovarian cancer by miRNA hsa-let-7d-3p[J].J Cancer Res Ther,2019,15(6):1321-1327.
6 Samadi P,Afshar S,Amini R,et al.Let-7e enhances the radiosensitivity of colorectal cancer cells by directly targeting insulin-like growth factor 1 receptor[J].J Cell Physiol,2019,234(7):10718-10725.
7 Siegel RL,Miller KD,Jemal A.Cancer statistics,2019[J].CA Cancer J Clin,2019,69(1):7-34.
8 Levy B,Hu ZI,Cordova KN,et al.Clinical utility of liquid diagnostic platforms in non-small cell lung cancer[J].Oncologist,2016,21(9):1121-1130.
9 Tang H,Du W,Jiang Y,et al.Upregulated expression of ROCK1 promotes cell proliferation by functioning as a target of miR-335-5p in non-small cell lung cancer[J].J Cell Physiol,2022,237(4):2299.
10 Wang X,Xiao H,Wu D,et al.miR-335-5p regulates cell cycle and metastasis in lung adenocarcinoma by targeting CCNB2[J].Onco Targets Ther,2020,13:6255-6263.
11 Zhu C,Wang S,Zheng M,et al.miR-31-5p modulates cell progression in lung adenocarcinoma through TNS1/p53 axis[J].Strahlenther Onkol,2022,198(3):304-314.
12 Xiang Q,Tang H,Yu J,et al.MicroRNA-98 sensitizes cisplatin-resistant human lung adenocarcinoma cells by up-regulation of HMGA2[J].Pharmazie,2013,68(4):274-281.
13 Yan Y,Zhang F,Fan Q,et al.Breast cancer-specific TRAIL expression mediated by miRNA response elements of let-7 and miR-122[J].Neoplasma,2014,61(6):672-679.
14 Song FC,Yang Y,Liu JX.Expression and significances of MiRNA Let-7 and HMGA2 in laryngeal carcinoma[J].Eur Rev Med Pharmacol Sci,2016,20(21):4452-4458.
15 Lovnicki J,Gan Y,Feng T,et al.LIN28B promotes the development of neuroendocrine prostate cancer[J].J Clin Invest,2020,130(10):5338-5348.
16 Chirshev E,Suzuki T,Wang H,et al.Let-7i reduces aggressive phenotype and induces BRCAness in ovarian cancer cells[J].Cancers(Basel),2021,13(18):4617.
17 Xu H,Liu C,Zhang Y,et al.Let-7b-5p regulates proliferation and apoptosis in multiple myeloma by targeting IGF1R[J].Acta Biochim Biophys Sin(Shanghai),2014,46(11):965-972.
18 Zheng S,Liu Q,Ma R,et al.Let-7b-5p inhibits proliferation and motility in squamous cell carcinoma cells through negative modulation of KIAA1377[J].Cell Biol Int,2019,43(6):634-641.
19 Chen T,Wu H,Chen X,et al.p53 mediates gnRH secretion via lin28/let-7 system in GT1-7 cells[J].Diabetes Metab Syndr Obes,2020,13:4681-4688.
20 Rong J,Xu L,Hu Y,et al.Inhibition of let-7b-5p contributes to an anti-tumorigenic macrophage phenotype through the SOCS1/STAT pathway in prostate cancer[J].Cancer Cell Int,2020,20:470.
21 Huang Y,Yu M,Kuma A,et al.Downregulation of let-7 by electrical acupuncture increases protein synthesis in mice[J].Front Physiol,2021,12:697139.
22 Liu X,Zeng W,Zheng D,et al.Let-7c-5p restrains cell growth and induces apoptosis of lung adenocarcinoma cells via targeting ESPL1[J].Mol Biotechnol,2022,64(12):1367-1375.
23 Al-Khafaji AS,Davies MP,Risk JM,et al.Aurora B expression modulates paclitaxel response in non-small cell lung cancer[J].Br J Cancer,2017,116(5):592-599.
24 Berger AC,Korkut A,Kanchi RS,et al.A comprehensive pan-cancer molecular study of gynecologic and breast cancers[J].Cancer Cell,2018,33(4):690-705.
25 Wang L,Xiao X,Du H.The regulation of let-7c-5p on the biological characteristics of lung adenocarcinoma cells by targeting AURKB[J].Mol Biotechnol,2022,64(5):526-534.
26 Dai CH,Chen P,Li J,et al.Co-inhibition of pol θ and HR genes efficiently synergize with cisplatin to suppress cisplatin-resistant lung cancer cells survival[J].Oncotarget,2016,7(40):65157-65170.
27 Shinmura K,Kato H,Kawanishi Y,et al.POLQ overexpression is associated with an increased somatic mutation load and PLK4 overexpression in lung adenocarcinoma[J].Cancers(Basel),2019,11(5):722.
28 Zhao XC,Wang GZ,Wen ZS,et al.Systematic identification of CDC34 that functions to stabilize EGFR and promote lung carcinogenesis[J].EBioMedicine,2020,53:102689.
29 Rahman MA,Amin AR,Wang D,et al.RRM2 regulates Bcl-2 in head and neck and lung cancers:a potential target for cancer therapy[J].Clin Cancer Res,2013,19(13):3416-3428.
30 Jin CY,Du L,Nuerlan AH,et al.High expression of RRM2 as an independent predictive factor of poor prognosis in patients with lung adenocarcinoma[J].Aging(Albany NY),2020,13(3):3518-3535.
31 Tang B,Xu W,Wang Y,et al.Identification of critical ferroptosis regulators in lung adenocarcinoma that RRM2 facilitates tumor immune infiltration by inhibiting ferroptotic death[J].Clin Immunol,2021,232:108872.
32 Meder L,König K,Dietlein F,et al.LIN28B enhanced tumorigenesis in an autochthonous KRAS(G12V)-driven lung carcinoma mouse model[J].Oncogene,2018,37(20):2746-2756.

[1]	TANG Dong, GAO Wenhong, ZHANG Huaping, CHEN Qianxue. Effects of morusin on the migration and invasion of U87 cells [J]. Journal of Practical Oncology, 2024, 38(3): 168-172.
[2]	BO Yun, HANG Wenlu, ZUO Shurun, Dong Yuan, LI Haiquan. Effects of long non-coding RNA OTUD6B-AS1 on proliferation,invasion and migration of lung adenocarcinoma A549 cells [J]. Journal of Practical Oncology, 2024, 38(1): 13-17.
[3]	ZHANG Xuan, LI Xin, ZHAO Kang. Relationship between serum inflammatory markers and prognosis of advanced non-small cell lung cancer patients treated with first-line immunosuppressants [J]. Journal of Practical Oncology, 2024, 38(1): 37-44.
[4]	XIE Junling. Research progress of activated leukocyte adhesion molecule in breast cancer [J]. Journal of Practical Oncology, 2024, 38(1): 45-49.
[5]	LU Yajie, WANG Lihong. Advances in early biomarkers of anthracyclines related to cardiotoxicity [J]. Journal of Practical Oncology, 2023, 37(6): 496-501.
[6]	ZHAO Shilu, PANG Da. Bioinformatics screening and analysis of prognostic genes in breast cancer [J]. Journal of Practical Oncology, 2023, 37(5): 422-428.
[7]	CAO Wenyuan, LUO Xiaoting, HE Qian. Research progress of exosomal miRNA in Clinical diagnosis of lymph node metastasis in thyroid cancer [J]. Journal of Practical Oncology, 2023, 37(5): 434-438.
[8]	FU Xinya, HU Lingdan, ZHANG Zhijian, MA Xiumei. HPA promotes the invasion and metastasis of human gastric cancer cells by up-regulating MCP1/CCR2 [J]. Journal of Practical Oncology, 2023, 37(4): 329-336.
[9]	MIAO Ling, ZHAO Zhihan, WANG Min. Levels and clinical significance of PA28γ and miR-7-5p in tumor tissues of patients with non-small cell lung cancer [J]. Journal of Practical Oncology, 2023, 37(4): 344-350.
[10]	CAO Yingyue, PANG Luyi, ZHANG Jian, JIN Shi. The role of cancer-associated fibroblasts in the progression of non-small cell lung cancer [J]. Journal of Practical Oncology, 2023, 37(4): 383-388.
[11]	CHEN Qi, HE Mingxv, JIA Keliang, MIAO Jianlong, HAN Liping. Research progress on the prognosis and treatment of non-small cell lung cancer with TP53-EGFR co-mutation [J]. Journal of Practical Oncology, 2023, 37(3): 262-266.
[12]	LONG Chaolian, LI Kun, LIU Zichen, ZHANG Nana, CHE Nanying. The correlation between the expression of programmed death ligand 1 in non-small cell lung cancer and clinical pathological features and common driver gene mutation [J]. Journal of Practical Oncology, 2023, 37(2): 117-122.
[13]	XIA Jianhong, ZHOU Liqing, YAN Yan, MA Tingting, SU Xinyu. The function and mechanism of lncRNA DUXAP8 in endometrial cancer cells [J]. Journal of Practical Oncology, 2023, 37(2): 137-143.
[14]	YU Yinping, LIN Jianan, WANG Lizhong, JIANG Weihua. Effects of cancer-derived exosomes on the biological beharior of breast cancer MCF-7 cells [J]. Journal of Practical Oncology, 2023, 37(1): 26-31.
[15]	ZHOU Junfeng, ZENG Zhiyao, LUO Yongfu, HE Chao. Effects of miR-765 targeting ARID1A on the invasion and migration of colorectal cancer cells [J]. Journal of Practical Oncology, 2023, 37(1): 32-38.

Hsa-let-7b-5p inhibits proliferation,migration and invasion in non-small cell lung cancer

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments