食管鳞癌中microRNA let-7a-3甲基化与IGF-Ⅱ的相关性

doi:10.11904/j.issn.1002-3070.2024.03.005

Abstract

Abstract: Objective The aim of this study was to explore the correlation between the methylation status of microRNA let-7a-3 in esophageal squamous cell carcinoma (ESCC)and the expression of insulin-like growth factor 2 (IGF-Ⅱ). Methods The methylation specific PCR (qMSP)was used to detect the methylation status of let-7a-3 in 83 cases of esophageal cancer and corresponding adjacent normal tissues.The enzyme linked immunosorbent assay (ELISA)was used to detect the expression of IGF-II in plasma. Results The degree of let-7a-3 methylation in cancer tissues of 83 patients with ESCC was significantly higher than that in normal tissues adjacent to cancer (P＜0.001).The expression of IGF-Ⅱ in the plasma of 83 patients with ESCC was positively correlated with the methylation degree of let-7a-3,which was statistically significant (r=0.600,P＜0.001). Conclusion microRNA let-7a-3 may participate in the occurrence and progression of ESCC by regulating the methylation of downstream molecules,which is of great significance for understanding the mechanisms of ESCC development and providing a basis for the diagnosis and prognosis of ESCC.

Key words: Esophageal squamous cell carcinoma, let-7a-3, Methylation, Insulin like growth factor 2

CLC Number:

R735.1

ZHU Gongjian, GUO Hongyun, BAI Yue, WANG Xiaomin, ZHU Xiaokang, LIU Dongyan, LIU Yuqin. The correlation between microRNA let-7a-3 methylation and IGF-Ⅱ expression in esophageal squamous cell carcinoma[J]. Journal of Practical Oncology, 2024, 38(3): 173-178.

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URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2024.03.005

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2024/V38/I3/173

References

1 赫捷,魏文强.2020中国肿瘤登记年报[M].北京:人民卫生出版社,2022.
2 Zhao Y,Xu L,Wang X,et al.A novel prognostic mRNA/miRNA signature for esophageal cancer and its immune[J].Mol Oncol,2021,15(4):1088-1109.
3 He FC,Meng WW,Qu YH,et al.Expression of circulating microRNA-20a and let-7a in esophageal squamous cell carcinoma[J].World J Gastroenterol,2015,21(15):4660-4665.
4 Ling R,Zhou Y,Zhou L,et al.Lin28/microRNA-let-7a promotes metastasis under circumstances of hyperactive Wnt[J].Mol Med Rep,2018,17(4):5265-5271.
5 Li D,Zhang L,Liu Y,et al.Specific DNA methylation markers in the diagnosis and prognosis of esophageal[J].Aging (Albany NY), 2019,11(23):11640-11658.
6 Gilyazova I,Ivanova E,Gilyazova G,et al.Methylation and expression levels of microRNA-23b/-24-1/-27b,microRNA-30c-1/-30e,microRNA-301a and let-7g are dysregulated in clear cell renal cell carcinoma[J].Mol Biol Rep,2021,48(7):5561-5569.
7 Chen Y,Xie C,Zheng X,et al.LIN28/let-7/PD-L1 Pathway as a target for cancer immunotherapy[J].Cancer Immunol Res,2019,7(3):487-497.
8 文利,彭睿,孙艳,等.microRNA let-7a-3基因甲基化与糖尿病肾病的关系[J].基础医学与临床,2016,36(4):474-479.
9 Reinhart BJ,Slack FJ,Basson M,et al.The 21-nucleotide let-7 RNA regulates developmental timing in caenorhabditis elegans[J].Nature,2000,403(6772):901-906.
10 Wells AC,Hioki KA,Angelou CC,et al.Let-7 enhances murine anti-tumor CD8 T cell responses by promoting memory and antagonizing terminal differentiation[J].Nat Commun.2023,14(1):5585.
11 Tonini T,Rossi F,Claudio PP,et al.Molecular basis of angiogenesis and cancer[J].Oncogene,2003,22(42):6549-6556.
12 Wang T,Wang G,Hao D,et al.Aberrant regulation of the LIN28A/LIN28B and let-7 loop in human malignant tumors and its effects on the hallmarks of cancer[J].Mol Cancer,2015,6(14):125.
13 Isanejad A,Alizadeh AM,Shalamzari SA,et al.MicroRNA-206,let-7a and microRNA-21 pathways involved in the anti-angiogenesis effects of the interval exercise training and hormone therapy in breast cancer[J].Life Sci,2016,4(151):30-40.
14 Buonfiglioli A,Efe IE,Guneykaya D,et al.let-7 MicroRNAs regulate microglial function and suppress glioma growth through Toll-Like receptor 7[J].Cell Rep,2019,29(11):3460-3471.
15 Wu T,Jia J,Xiong X,et al.Increased expression of Lin28B associates with poor prognosis in patients with oral squamous cell carcinoma[J].PLoS ONE,2013,8(12):e83869.
16 Wei W,Dong Z,Gao H,et al.MicroRNA-9 enhanced radiosensitivity and its mechanism of DNA methylation in non-small cell lung cancer[J].Gene,2019,710(20):178-185.
17 Zhang Y,Chen F,Zhang F,et al.Characterization of DNA methylation as well as mico-RNA expression and screening of epigenetic markers in adipogenesis[J].J Transl Med,2022,20(1):93.
18 Hong W,Xue M,Jiang J,et al.Circular RNA circ-CPA4/ let-7 miRNA/PD-L1 axis regulates cell growth,stemness,drug resistance and immune evasion in non-small cell lung cancer (NSCLC)[J].J Exp Clin Cancer Res,2020,39(1):149.
19 Filippova EA,Pronina IV,Lukina SS,et al.Relationship of the Levels of microRNA gene methylation with the level of their expression and pathomorphological characteristics of breast cancer[J].Bull Exp Biol Med,2021,171(6):764-769.
20 Kushlinskii NE,Utkin DO,Loginov VI,et al.Clinical significance of methylation of a group of miRNA genes in patients with ovarian cancer[J].Klin Lab Diagn,2020,65(5):321-327.
21 陈德玉,金丽艳,朱龙萍,等.let-7a和IL-6在食管鳞状细胞癌组织中的表达及两者的相关性[J].细胞与分子免疫学杂志,2013,29(11):1181-1184.
22 Brueckner B,Stresemann C,Kuner R,et al.The human let-7a-3 locus contains an epigenetically regulated microRNA gene with oncogenic function[J].Cancer Res,2007,67(4):1419-1423.
23 Wu DH,Yao DM,Yang L,et al.Hypomethylation of let-7a-3 is associated with poor prognosis in myelodysplastic syndrome[J].Leuk Lymphoma,2017,58(1):96-103.
24 Zhu XW,Yao DM,Wu DH,et al.Let-7a-3 hypomethylation is associated with favorable/intermediate karyotypes but not with survival in acute myeloid leukemia[J].Tumour Biol,2016,37(1):491-501.
25 Yura M,Fukuda K,Matsuda S,et al.Effects of let-7a microRNA and C-C chemokine receptor type 7 expression on cellular function and prognosis in esophageal squamous cell carcinoma[J].BMC Cancer,2022,22(1):1064.
26 李祥全,姜莹,丁华锋,等.血清Copeptin、IGF-Ⅱ联合APACHEⅡ评分对颅脑外伤患者预后的预测价值[J].国际神经病学神经外科学杂志,2021,48(3):231-234.
27 Giabicani E,Chantot-Bastaraud S,Bonnard A,et al.Roles of type 1 insulin-like growth factor (IGF)receptor and IGF-II in growth regulation:evidence from a patient carrying both an 11p paternal duplication and 15q deletion[J].Front Endocrinol (Lausanne),2019,30(10):263.
28 Holly JMP,Biernacka K,Perks CM.The neglected lnsulin:IGF-II,a metabolic regulator with implications for diabetes,obesity,and cancer[J].Cells,2019,8(10):1207.
29 Cacheux W,Lievre A,Richon S,et al.Interaction between IGF2-PI3K axis and cancer-associated-fibroblasts promotes anal squamous carcinogenesis[J].Int J Cancer,2019,145:1852-1859.
30 Zheng DN,Zhang CJ,Sun GP.Long non-coding RNA MNX1-AS1 promotes migration and invasion of esophageal squamous cell carcinoma by upregulating IGF2[J].Eur Rev Med Pharmacol Sci,2019,23:6179-6185.
31 Ioannidis P,Mahaira LG,Perez SA,et al.CRD-BP/IMP1 expression characterizes cord blood CD34⁺ stem cells and affects c-myc and IGF-II expression in MCF-7 cancer cells[J].J Biol Chem,2005,280(20):20086-20093.

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The correlation between microRNA let-7a-3 methylation and IGF-Ⅱ expression in esophageal squamous cell carcinoma

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