AEG-1、MMP-9在结直肠组织中的表达及临床病理相关性的研究

doi:10.11904/j.issn.1002-3070.2015.03.002

Abstract

Abstract:

Objective To investigate the expression of astrocyte elevated gene-1(AEG-1)and matrix metallopeptidase 9(MMP-9)in colorectal tissues and evaluate its correlation with clinical pathologic factors of colorectal cancer.Methods AEG-1 and MMP-9 expression in normal colorectal mucous(n=45),low-grade adenoma(n=31),high-grade adenoma(n=15)and colorectal carcinoma(n=146)were examined by immunohistochemistry.Statistical analyses were applied to test the significance of its expression.Results AEG-1 expression levels were gradually elevated in normal tissues,low-grade adenoma,high-grade adenoma and colorectal carcinoma respectively.Furthermore,there was a similar trend for MMP-9 from normal mucous to adenoma and carcinoma.Statistical analysis revealed that AEG-1 expression was markedly correlated with the UICC stage,T classification,N classification,M classification,and histological differentiation in the colorectal cancer patients,but not with age,gender,tumor location and tumor size.In addition,AEG-1 expression was positively correlated with the MMP-9 expression in colorectal cancer.Besides,those patients with high AEG-1 and MMP-9 levels had shorter survival time(P＜0.05).Conclusion AEG-1 may be involved in carcinogenesis and progression of colorectal carcinoma and can be a novel prognostic biomarker in patients with colorectal carcinoma.

Key words: Astrocyte elevated gene-1, Matrix metalloproteinase-9, Colorectal carcinoma, Colorectal adenoma

CLC Number:

R735.34

SONG Hongtao,QIN Yu,LI Cong,YAO Guodong,WANG Shuhuai,GENG Jingshu. Study on the association between expression and clinicopathological significance of AEG-1 and MMP-9 in colorectal tissue[J]. PRACTICAL ONCOLOGY JOURNAL, 2015, 29(3): 197-202.

References

1 Siegel R,Ma J,Zou ZH,et al.Cancer statistics,2014[J].CA Cancer J Clin,2014,64(1):9-29.
2 Fearon ER,Vogelstein B.A genetic model for colorectal tumorigenesis[J].Cell,1990,61(5):759-767.
3 Vogelstein B,Fearon ER,Hamilton SR,et al.Genetic alterations during colorectal-tumor development[J].New England Journal of Medicine,1988,319(9):525-532.
4 Carvalho B,Postma C,Mongera S,et al.Multiple putative oncogenes at the chromosome 20q amplicon contribute to colorectal adenoma to carcinoma progression[J].Gut,2009,58(1):79-89.
5 刘运铎,孟凡玲,徐冶,等.AEG-1、DLL4在子宫内膜癌中的表达及临床病理相关性的研究[J].实用肿瘤学杂志,2013,27(3):223-228.
6 Lee SG,Su ZZ,Emdad L,et al.Astrocyte elevated gene-1(AEG-1)is a target gene of oncogenic Ha-ras requiring phosphatidylinositol 3-kinase and c-Myc[J].Proc Natl Acad Sci USA,2006,103(46):17390-17395.
7 Emdad L,Sarkar D,Su ZZ,et al.Activation of the nuclear factor kappaB pathway by astrocyte elevated gene-1:implications for tumor progression and metastasis[J].Cancer Res,2006,66(3):1509-1516.
8 Emdad L,Sarkar D,Su ZZ,et al.Astrocyte elevated gene-1:recent insights into a novel gene involved in tumor progression,metastasis and neurodegeneration[J].Pharmacol Ther,2007,114(2):155-170.
9 Sarkar D,Park ES,Emdad L,et al.Molecular basis of nuclear factor-kappaB activation by astrocyte elevated gene-1[J].Cancer Res,2008,68(5):1478-1484.
10 Hu G,Chong RA,Yang Q,et al.MTDH activation by 8q22 genomic gain promotes chemoresistance and metastasis of poor-prognosis breast cancer[J].Cancer Cell,2009,15(1):9-20.
11 Li J,Zhang N,Song LB,et al.Astrocyte elevated gene-1 is a novel prognostic marker for breast cancer progression and overall patient survival[J].Clin Cancer Res,2008,14(11):3319-3326.
12 刘力,谭米多,戴洪波,等.MMP-9和E-cadherin在乳腺癌中的表达及其临床意义[J].肿瘤药学,2013,3(3)180-184.
13 Samowitz WS,Slattery ML,Sweeney C,et al.APC mutations and other genetic and epigenetic changes in colon cancer[J].Molecular Cancer Research,2007,5(2):165-170.
14 Rex DK,Kahi CJ,Levin B,et al.Guidelines for colonoscopy surveillance after cancer resection:a consensus update by the American Cancer Society and US Multi-Society Task Force on Colorectal Cancer[J].CA Cancer J Clin,2006,56(3),160-167.
15 Yoo BK,Emdad L,Su ZZ,et al.Astrocyte elevated gene-1 regulates hepatocellular carcinoma development and progression[J].J Clin Invest,2009,119(3),465-477.
16 Liu L,Wu J,Ying Z,et al.Astrocyte elevated gene-1 upregulates matrix metalloproteinase-9 and induces human glioma invasion[J].Cancer Res,2010,70(9):3750-3759.
17 Illemann M,Bird N,Majeed A,et al.MMP-9 is differentially expressed in primary human colorectal adenocarcinomas and their metastases[J].Mol Cancer Res,2006,4(5):293-302.

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Study on the association between expression and clinicopathological significance of AEG-1 and MMP-9 in colorectal tissue

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