Abstract: Objective To investigate the expression of Mina53 and Ki-67 in the human colorectal carcinoma,and the correlation between the expression and the clinicopathology,study the clinical significance of Mina53 and Ki-67 in colorectal carcinoma,and afford a new molecule biological evidence and method for earlier diagnosis,prevention and therapy of colorectal cancer.Method 82 specimens of different colorectal carcinoma tissue were collected by surgery in the first people′s hospital in Jingzhou between Dec.2004 and Dec.2006.All of the specimens were detected with the immunhistochemical method of SP by the Mina53 antibody and the Ki-67 antibody,respectively.The relationship between the expression of Mina53,Ki-67 and the clinicopathology were analyed by SPSS13.0.Results The positive rates of the expression of Mina53 and Ki-67 were 78.05%(64/82) and 80.49%(66/82)in human colorectal carcinoma,respectively,which were significant higher than those in adjacent nonneoplastic tissues.The expression of Mina53 and Ki-67 was related to the Duke′s stage,the P value is 0.029 and 0.037,respectively.There was no obvious correlation between the expression and the age,tumor size and the pathological grade;univariate analysis of Cox proportional hazards regression model revealed that the expression of Mina53 and the stage were relevant to prognosis,the P value was 0.039 and 0.000,respectively.Conclusion Overexpressions of Mina53 and Ki-67 were found in the colorectal carcinoma compared with no expression in the adjacent nonneoplastic tissues,and this maybe illustrate the invasion and metastasis of the tumor.Mina53 maybe serve as a prognostic factor of colorectal carcinoma.