LncRNA SOX2OT海绵吸附miR-34a促进SOX2表达增强大肠癌多药耐药

doi:10.11904/j.issn.1002-3070.2020.05.003

Abstract

Abstract: Objective The aim of this study was to verify that long noncoding RNA SOX2OT(SOX2OT)enhanced the multidrug resistance(MDR)of colorectal cancer HCT-116 cells,and explored its possible effective mechanism on SOX2OT/miR-34a/SOX2 signaling pathway.Methods Dual luciferase reporter gene assay was used to verify the targeted binding of SOX2OT to miR-34a,and miR-34a to SOX2;The SOX2OT plasmid and miR-34a were transient transfected into HCT-116 cells,and the transfection efficiency was verified by RT-qPCR;RT-qPCR was used to detect the levels of SOX2OT,miR-34a and SOX2,and the correlation amongst them were analyzed;The CCK-8 assay was used to detect cell proliferation and to assess the influence of SOX2OT and miR-34a on the chemosensitivity of HCT-116 cells to 5-fluorouracil(5-FU),vincristine(VCR),cisplatin(CDDP),and paclitaxel(Taxol).Results There was a targeting relationship between SOX2OT and miR-34a,and miR-34a with SOX2;The SOX2OT inhibited miR-34a(P＜0.05)and upregulated the level of SOX2(P＜0.01);The MiR-34a down-regulated the expression of SOX2OT and SOX2(P＜0.01);The IC₅₀ values for 5-FU,VCR,CDDP,and TAXOL in HCT-116 cells were increased after transfection of SOX2OT plasmid(P＜0.01);The IC₅₀ values of four chemotherapy drugs in the co-transfected with SOX2OT and miR-34a mimic groups were significantly lower than those of the transfected SOX2OT group(P＜0.01).The IC₅₀ values of 5-FU and CDDP were not different from the control group(P＞0.05).Conclusion The SOX2OT sponge adsorbs miR-34a and promotes the expression of SOX2,resulting in multidrug resistance of HCT-116 cells.

Key words: Colorectal cancer, Multidrug resistance, SOX2OT, miR-34a

CLC Number:

R735.3⁺4

GUO Piaoting, ZOU Yang. LncRNA SOX2OT sponge adsorbs miR-34a to promote SOX2 expression and enhance multidrug resistance in colorectal cancer[J]. Journal of Practical Oncology, 2020, 34(5): 398-403.

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URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2020.05.003

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2020/V34/I5/398

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LncRNA SOX2OT sponge adsorbs miR-34a to promote SOX2 expression and enhance multidrug resistance in colorectal cancer

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